Quantitative analysis of surface marker densities after exposure of T-cells to concanavalin A (Con A): A sensitive early index of cellular activation

Shabtai, Moshe; Malinowski, Kazimierz; Waltzer, Wayne C.; Pullis, Christopher; Raisbeck, Audrey P.; Rapaport, Felix T.

doi:10.1016/0008-8749(91)90123-s

Cited by 7 publications

(2 citation statements)

References 22 publications

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“…These findings indicate that the immune reaction in the CSF of TBE patients is a dynamic process. Therefore the results of immune parameters in CSF may not be simply explained in the terms of the "in vitro" lymphocyte activation (31,32). Our results may only suggest a multistep immune process in TBE disease, where non-specifically activated HLA-DR cells are gradually replaced by antigen-stimulated CD25+ cells.…”

Section: Discussionmentioning

confidence: 74%

Flow cytometric analysis of lymphocytes in cerebrospinal fluid in patients with tick-borne encephalitis

Tomažič

Ihan

1997

Acta Neurologica Scandinavica

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Section: Discussionmentioning

confidence: 74%

Flow cytometric analysis of lymphocytes in cerebrospinal fluid in patients with tick-borne encephalitis

Tomažič

Ihan

1997

Acta Neurologica Scandinavica

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“…The recent application of flow-cytometric techniques of immunophenotyping to the study of human disease has permitted the quantitation of relative concentrations of various biologically active receptor markers on the lymphocyte surface [36,37]. Lymphocyte development, maturation and aging occur in maj or lymphocyte populations and their subsets from birth, through adulthood and aging.…”

Section: Immune Function In the Elderly Patientmentioning

confidence: 99%

Dialysis access, immune function and renal transplantation in the elderly

Waltzer

Shabtai

Malinowski

et al. 1994

Geriatric Nephrol Urol

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CD4+CD25+ regulatory T cells in rheumatoid arthritis: Differences in the presence, phenotype, and function between peripheral blood and synovial fluid

Amelsfort¹,

Jacobs²,

Bijlsma³

et al. 2004

Arthritis & Rheumatism

473

353

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Objective. In mice, CD4؉CD25؉ regulatory T cells play a pivotal role in preventing autoimmunity. Regulatory T cells are also present and functional in healthy humans. We investigated the presence, phenotype, and function of CD4؉CD25؉ regulatory T cells in peripheral blood (PB) and synovial fluid (SF) from patients with rheumatoid arthritis (RA).Methods. The presence and phenotype of CD4؉CD25؉ regulatory T cells were determined by flow cytometry. Anergy and suppressive activity were assessed by culturing CD4؉CD25؊ and CD4؉CD25؉ T cells with anti-CD3 monoclonal antibodies and antigen-presenting cells, followed by proliferation and cytokine detection.Results. The percentage of CD4؉CD25؉ T cells in RA SF was significantly increased compared with that in RA PB, and both of these percentages were higher than that in PB from controls. The cells in RA PB were similar in phenotype and function to CD4؉CD25؉ regulatory T cells from controls. In SF, however, ϳ40-50% of CD4؉CD25؉ T cells expressed an activated phenotype, i.e., CD69؉, class II MHC؉, OX-40؉, with high levels of CTLA-4 and glucocorticoid-induced tumor necrosis factor receptor. These synovial CD4؉CD25؉ T cells displayed an increased suppressive capacity compared with blood CD4؉CD25؉ T cells. However, this enhanced suppressive activity was counterbalanced, because activated responder T cells from SF were less susceptible to CD4؉CD25؉ T cell-mediated suppression than were responder cells from PB.Conclusion. We demonstrate that CD4؉CD25؉ regulatory T cells are present and functional in patients with RA, with higher numbers of regulatory T cells with increased suppressive activity found in SF compared with PB. These findings suggest a negative feedback system that is active at the site of inflammation. The balance between activated responder and regulatory T cells appears to influence the extent of immunoregulation in RA.

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Quantitative analysis of surface marker densities after exposure of T-cells to concanavalin A (Con A): A sensitive early index of cellular activation

Cited by 7 publications

References 22 publications

Flow cytometric analysis of lymphocytes in cerebrospinal fluid in patients with tick-borne encephalitis

Flow cytometric analysis of lymphocytes in cerebrospinal fluid in patients with tick-borne encephalitis

Dialysis access, immune function and renal transplantation in the elderly

CD4+CD25+ regulatory T cells in rheumatoid arthritis: Differences in the presence, phenotype, and function between peripheral blood and synovial fluid

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