Quantitative Analysis of Total Phenolic Contents and Antioxidant Activity of Fresh Field Natural Rubber Latex

Abstract

Summary: Dried films of fresh field natural rubber latex from Pattani local rubber plantation, Thailand were prepared. The film (1g) was dissolved by various solvent (100ml) i.e. hexane, chloroform and mixtures of chloroform: methanol in the ratio of 1:1, 2:1 and 4:1. The solution was purified by separated the rubber out with methanol. The non-rubber solution was then quantitative analyzed to verify total phenolic content (TPC), ferric reducing antioxidant power (FRAP) and DPPH radical scavenging activity using Oxicount Antioxidant kit test. It was found that only hexane, chloroform and mixture of chloroform: methanol of ratio 4:1 can dissolve rubber film completely. The non-rubber content yields after evaporated solvent were 8. .42 AE 0.05%, respectively. This study found that TPC and antioxidant activity of rubber film clearly depend on the type of dissolved solvent. The highest antioxidant activity from this study was found by using the mixture of chloroform: methanol in the ratio of 4:1. Furthermore, the phenolic acid content of natural rubber were reported using HPLC, based on which more phenolic acids were presented in the sample extracted by chloroform:methanol mixture.

Free-radical Scavenging properties and Cytotoxic Activity Evaluation of Latex C-serum from Hevea brasiliensis RRIM 600

“…4,28 Amnuaikit and Boonme (2014) 14 also evaluated C-serum latex from H. brasiliensis through the reduction method of DPPH and found antioxidant activity. Siriwong et al (2015) 15 detected phenolic compounds in the non-rubber fraction of H. brasiliensis latex and observed that it exerts antioxidant activity through FRAP and DPPH reduction assays. The results found in this study show that different concentrations of latex C-serum from H. brasiliensis present scavenging properties against hydroxyl and nitric oxide radicals and hydrogen peroxide (Figure 1) and the scavenging ability of latex C-serum was compared to a standard non-enzymatic antioxidant compound, ascorbic acid.…”

“…Some few in vitro studies reported that H. brasiliensis latex C-serumhas antioxidant properties and this factopens new perspectives about possible biomedical applications. 14,15 One of the possibilities could be the use of H. brasiliensis latex C-serumas adjuvantin antioxidant therapies. Another possibility could be its use in preventive medicine and esthetics.…”