Quantitative Analysis of Uric Acid Metabolites in Urine by High Performance Liquid Chromatography - Mass Spectrometry Using Silica Hydride Columns

Young, Joshua E.; Pesek, Joseph J.; Matyska, Maria T.; Sanchez, B; White, Brandon

doi:10.2174/2213240604666170106160806

Cited by 4 publications

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“…One study demonstrated how this column could be used to analyze a number of drugs of abuse [33]. Another investigation involved separating and analyzing uric acid cycle metabolites in the ANP mode where pre-column derivatization is not necessary as in many reversed-phase methods [51]. The diol phase was also included in a comparative study four different silica hydride phases [32].…”

Section: Diolmentioning

confidence: 99%

Silica Hydride: A Separation Material Every Analyst Should Know About

Pesek

Matyska

2021

Molecules

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This review describes the development, special features and applications of silica hydride-based stationary phases for HPLC. The unique surface of this material is in contrast to ordinary, standard silica, which is the material most frequently used in modern HPLC stationary phases. The standard silica surface contains mainly silanol (Si-OH) groups, while the silica hydride surface is instead composed of silicon-hydrogen groups, which is much more stable, less reactive and delivers different chromatographic and chemical characteristics. Other aspects of this material are described for each of the different bonded moieties available commercially. Some applications for each of these column types are also presented as well as a generic model for method development on silica hydride-based stationary phases.

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Section: Diolmentioning

confidence: 99%

Silica Hydride: A Separation Material Every Analyst Should Know About

Pesek

Matyska

2021

Molecules

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“…Hence, in the present work it is proposed the use of EC-SOERS to determine uric acid (UA) in relevant samples (synthetic urine). Although there are numerous techniques to determine uric acid, such as colorimetric enzymatic assays [27][28][29][30], liquid chromatography [30][31][32] or even SERS [21] or EC-SERS [19]. These techniques…”

Section: Accepted Manuscriptmentioning

confidence: 99%

“…Uric acid has been determined in urine in very wide ranges of concentration, from 50 nM to 3.6 mM, using very different analytical techniques [32][33][34][35]. Particularly, using…”

Section: Accepted Manuscriptmentioning

confidence: 99%

Determination of uric acid in synthetic urine by using electrochemical surface oxidation enhanced Raman scattering

Hernandez

Perales-Rondón

Heras

et al. 2019

Analytica Chimica Acta

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In this work, a new and easy methodology to determine uric acid in relevant samples using Raman spectroelectrochemistry is presented. The spectroelectrochemistry experiment is based on the in-situ formation of a suitable substrate that enables the enhancement of the Raman signal of an analyte during the oxidation stage of a silver electrode. This phenomenon is known as electrochemical surface oxidation enhanced Raman scattering (EC-SOERS) and has proved to be useful in quantitative analysis using disposable screen printed electrodes. The successful combination of EC-SOERS with PARAFAC analysis allows the determination of uric acid in a relevant complex sample avoiding the use of standard addition method and without using a baseline correction, which simplifies the application of such methodology in routine analysis.

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“…Several analytical methods have been reported for UA detection, for example, liquid chromatography, [4][5][6] UV-vis, fluorescence spectroscopy, [7][8][9][10][11] UV-vis spectrophotometry, 12,13 high-efficiency liquid chromatography, [14][15][16][17][18][19] colorimetry, [20][21][22][23][24][25] capillary electrophoresis, [26][27][28] and chemoluminescence [29][30][31][32] however, most of them have some drawbacks, highlighting the need for a sample pretreatment stage to avoid interferences in the analytical signal, high instrumentation cost, and in some cases the use of organic solvents. 33 Electrochemical-based methodologies have arisen as viable alternatives in the quantification of UA, overcoming the drawbacks mentioned above due to its low-cost compared to other techniques, rapid response, and easy implementation.…”

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confidence: 99%

Review—Trends on the Development of Non-Enzymatic Electrochemical Sensors Modified with Metal-Oxide Nanostructures for the Quantification of Uric Acid

Ramírez

Mendoza-Huízar

Galán-Vidal

et al. 2021

J. Electrochem. Soc.

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Uric acid is one of the most important metabolites as its blood levels can help to diagnose important diseases. Tradicionally, uric acid is determined by enzymatic-spectrophotometric techniques, but in recent years new promising alternatives have arisen. This review is devoted to the development over the past decade of non-enzymatic electrochemical studies based on nano-structures of non-noble metal oxides (Fe2O3, CuO, Cu2O, ZnO, NiO, MnO2, CeO2, MgO, SnO2 and Co3O4) for uric acid detection. The proposals showed the application of electrochemical sensors for the determination of uric acid in blood, urine, pharmaceuticals, water, and commercial shellfish. The developed electrodes are based on vitreous carbon electrodes, carbon paste, or screen-printed, mainly modified with nano-structures of metal oxides to electrochemically oxidize uric acid, where the anodic current peak is used as the analytical signal and the results reported are very promising.

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Quantitative Analysis of Uric Acid Metabolites in Urine by High Performance Liquid Chromatography - Mass Spectrometry Using Silica Hydride Columns

Cited by 4 publications

References 0 publications

Silica Hydride: A Separation Material Every Analyst Should Know About

Silica Hydride: A Separation Material Every Analyst Should Know About

Determination of uric acid in synthetic urine by using electrochemical surface oxidation enhanced Raman scattering

Review—Trends on the Development of Non-Enzymatic Electrochemical Sensors Modified with Metal-Oxide Nanostructures for the Quantification of Uric Acid

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