Quantitative and qualitative morphologic, cytochemical, and ultrastructural characteristics of blood cells in captive Asian water monitors

Salakij, Chaleow; Salakij, Jarernsak; Prihirunkit, Kreangsak; Narkkong, Naul-Anong; Sanyathitiseree, Pornchai; Kranjanapitukkul, Kwanjai

doi:10.1111/vcp.12183

Cited by 21 publications

(66 citation statements)

References 9 publications

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“…The MCV of the BSK was smaller than that of the BrK, BK, painted stork, lesser adjutant, greater adjutant, great argus, and crested serpent eagle ( Spilornis cheela ) . The morphometric analysis of RBCs in the three kite species of this study showed a correlation with the MCV.…”

Section: Discussionmentioning

confidence: 47%

“…Differential leukocyte (white blood cell, WBC) counts were assessed by counting 200 cells, and the final data represent the averaged counts between two clinicians. The total erythrocyte (red blood cell, RBC) and WBC counts were determined manually with the improved Neubauer counting chamber after 5 µL of blood were diluted by mixing with 1 mL of a Natt and Herrick solution to obtain a 1:200 solution as described previously . As thrombocytes in the Natt and Herrick solution were difficult to differentiate from lymphocytes, the number of thrombocytes per microliter of blood was not determined.…”

Section: Methodsmentioning

confidence: 99%

“…Examination of blood films is an important component of hematologic investigations and provides useful information about blood cell alterations . There are some reports on the morphology and ultrastructure of avian blood cells in Thailand and hemograms from other raptors; however, variations in cell characteristics exist among species within the Aves class . Normal hematologic values vary between the species as well .…”

Section: Introductionmentioning

confidence: 99%

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Quantitative and qualitative characteristics of blood cells in black‐shouldered, Brahminy, and black kites

Salakij

Kasorndorkbua

Pornpanom

et al. 2019

Veterinary Clinical Pathol

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Background: Black-shouldered kites (BSK, Elanus caeruleus), Brahminy kites (BrK, Haliastur indus), and black kites (BK, Milvus migrans govinda) are medium-sized hawks found in Thailand, and little is known about the hematology of these three kite species.Objective: This study reports basic hematologic values and describes the light microscopic, cytochemical, and ultrastructural characteristics of blood cells in these kites.Methods: Blood samples were collected from 113 healthy kites (50 BSKs, 53 BrKs, and 10 BKs) from January 2012 to December 2017. Complete blood cell counts, cytochemical staining (Sudan black B, peroxidase [PO], periodic acid-Schiff, α-naphthyl acetate esterase, and β-glucuronidase), and transmission electron microscopy were performed using standard methods.Results: Hematology, morphometry, and cytochemical staining patterns of blood cells were tabulated. BSK erythrocytes were smaller than BrK and BK erythrocytes.Heterophils, the largest granulocytes, were the most prevalent leukocytes in all kites. Cytochemical reactions in blood cells from these three kite species were the same, except that heterophils from BrKs were the only cells positive for PO. The ultrastructure of heterophil and eosinophil granules from the BSKs were similar in their homogeneous electron densities but differed in shape. The eosinophil granules from BrKs and BKs revealed heterogeneous electron densities with central pallor in some granules. Basophils had different granular electron densities, and some granules were electron-lucent. Conclusion:The 23 baseline hematologic values and morphologic, cytochemical, and ultrastructural characteristics of all blood cell types in this study provide reference data for future kite healthcare. K E Y W O R D S Accipitridae, beta-glucuronidase, cytochemistry, hematology, ultrastructure 1 | INTRODUCTION The black-shouldered or black-winged kite (Elanus caeruleus, BSK), Brahminy kite (Haliastur indus, BrK), and black kite (Milvus migrans govinda, BK) are medium-sized hawks of the family Accipitridae (order Accipitriformes) and are the only three kite species commonly found in Thailand. The BSK and BrK are common residents, whereas the BK is a rare resident but common winter visitor. 1 The Kasetsart University Raptor Rehabilitation Unit (KURRU) has admitted various

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Section: Discussionmentioning

confidence: 47%

Section: Methodsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Quantitative and qualitative characteristics of blood cells in black‐shouldered, Brahminy, and black kites

Salakij

Kasorndorkbua

Pornpanom

et al. 2019

Veterinary Clinical Pathol

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“…This suggests that hemogregarine infection was associated with an enhanced erythropoiesis, most likely due to low‐grade hemolysis. Hemogregarine parasite infection is usually reported as nonpathogenic in reptiles, and unassociated with decreased PCV or RBC counts . The hemogregarine Hemolivia mariae has been reported to infect Bobtail lizards in South Australia .…”

Section: Discussionmentioning

confidence: 99%

“…Hemogregarine parasite infection is usually reported as nonpathogenic in reptiles, and unassociated with decreased PCV or RBC counts. 17,18 The hemogregarine Hemolivia mariae has been reported to infect Bobtail lizards in South Australia. 13 Further studies to identify the causative hemogregarine species and assess its pathogenicity in Western Australian Bobtail lizards are warranted.…”

Section: Discussionmentioning

confidence: 99%