Quantitative Angle-Resolved SPR Imaging of DNA−DNA and DNA−Drug Kinetics

Wolf, Lauren K.; Fullenkamp, Dominic E; Georgiadis, R.

doi:10.1021/ja056422w

Cited by 86 publications

(73 citation statements)

References 27 publications

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“…If the target and probe interact in a simple 1:1 ratio, then in the absence of bulk transport the surface reaction can be represented in the form: (1) where TP is the surface bound target-probe complex (see also Figure 3 inset). An example of such a reaction would be the adsorption of an ssDNA 16mer target molecule onto a surface bound ssDNA 16mer probe molecule to form a surface bound dsDNA duplex (we define this process as "hybridization adsorption").…”

Section: A Langmuir Adsorption and Desorption Kineticsmentioning

confidence: 99%

Creating Advanced Multifunctional Biosensors with Surface Enzymatic Transformations

2006

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This article summarizes our recent work on the coupling of surface enzyme chemistry and bioaffinity interactions on biopolymer microarrays for the creation of multiplexed biosensors with enhanced selectivity and sensitivity. The surface sensitive techniques of surface plasmon resonance imaging (SPRI) and surface plasmon fluorescence spectroscopy (SPFS) are used to detect the surface enzymatic transformations in real time. Three specific examples of novel coupled surface bioaffinity/ surface enzymatic processes are demonstrated: (i) a surface enzymatic amplification method utilizing the enzyme ribonuclease H (RNase H) in conjunction with RNA microarrays that permits the ultrasensitive direct detection of genomic DNA at a concentration of 1 fM without labeling or PCR amplification, (ii) the use of RNA-DNA ligation chemistry to create renewable RNA microarrays from single stranded DNA microarrays, and (iii) the application of T7 RNA polymerase for the onchip replication of RNA from double stranded DNA microarray elements. In addition, a simple yet powerful theoretical framework that includes the contributions of both enzyme adsorption and surface enzyme kinetics is used to quantitate surface enzyme reactivity. This model is successfully applied to SPRI and SPFS measurements of surface hydrolysis reactions of RNase H and Exonuclease III (Exo III) on oligonucleotide microarrays.

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Section: A Langmuir Adsorption and Desorption Kineticsmentioning

confidence: 99%

Creating Advanced Multifunctional Biosensors with Surface Enzymatic Transformations

2006

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“…Surface plasmon resonance (SPR) [19,20] has been demonstrated to be a powerful and simple optical technique for bioaffinity studies at the solid/solution interface [21][22][23][24][25][26]. The technique is based on measuring the change in refractive index accompanying the binding of a solution species with a species preimmobilized onto a metal-film surface [20].…”

Section: Introductionmentioning

confidence: 99%

Comparative studies of the interaction between ferulic acid and bovine serum albumin by ACE and surface plasmon resonance

Zhang

et al. 2007

Electrophoresis

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Affinity capillary electrophoresis (ACE) was used to study the interaction between ferulic acid (FA) and BSA. The interaction between FA and BSA was facilitated by injecting FA into a BSA-containing running buffer. Both mobility ratio and mobility shift assays were performed to deduce the binding constant (K(b)). However, the K(b )value obtained with the mobility ratio assay was only approximately 20% of that extracted from the mobility shift assay. The former assay yielded a K(b) value (5.6 +/- 0.4 x 10(4) M(-1)), which compares well with the result obtained with surface plasmon resonance (SPR) (5.1 +/- 0.6 x 10(4) M(-1)). The discrepancy between the mobility ratio and mobility shift assays suggests that the data extrapolation from the mobility ratio should be more reliable for cases when both changes in the EOF and viscosity of the running buffer are important. The work demonstrates that ACE, a solution-based technique, and SPR, a technique addressing interfacial processes, are highly complementary to each other and the comparative studies are confirmatory and allow binding constants to be accurately determined.

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“…This is when imaging technology comes in. Wolf et al [21,22] developed a prism-and detectionarm-rotated angle-resolved SPRi for quantitative characterization of DNA interactions and the kinetics of DNA-drug binding. White light from a tungsten-halogen source was used for SPR excitation, and a liquid crystal variable retarder was employed to correct images for spatial intensity variation in the light source.…”

Section: Angle Interrogation Sprmentioning

confidence: 99%

Recent advances in surface plasmon resonance imaging: detection speed, sensitivity, and portability

et al. 2017

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Surface plasmon resonance (SPR) biosensor is a powerful tool for studying the kinetics of biomolecular interactions because they offer unique real-time and label-free measurement capabilities with high detection sensitivity. In the past two decades, SPR technology has been successfully commercialized and its performance has continuously been improved with lots of engineering efforts. In this review, we describe the recent advances in SPR technologies. The developments of SPR technologies focusing on detection speed, sensitivity, and portability are discussed in details. The incorporation of imaging techniques into SPR sensing is emphasized. In addition, our SPR imaging biosensors based on the scanning of wavelength by a solid-state tunable wavelength filter are highlighted. Finally, significant advances of the vast developments in nanotechnology-associated SPR sensing for sensitivity enhancements are also reviewed. It is hoped that this review will provide some insights for researchers who are interested in SPR sensing, and help them develop SPR sensors with better sensitivity and higher throughput.

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Quantitative Angle-Resolved SPR Imaging of DNA−DNA and DNA−Drug Kinetics

Cited by 86 publications

References 27 publications

Creating Advanced Multifunctional Biosensors with Surface Enzymatic Transformations

Creating Advanced Multifunctional Biosensors with Surface Enzymatic Transformations

Comparative studies of the interaction between ferulic acid and bovine serum albumin by ACE and surface plasmon resonance

Recent advances in surface plasmon resonance imaging: detection speed, sensitivity, and portability

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