1999
DOI: 10.1006/abio.1999.4069
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Quantitative Aspects of the Use of Bacterial Chloramphenicol Acetyltransferase as a Reporter System in the YeastSaccharomyces cerevisiae

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Cited by 6 publications
(1 citation statement)
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“…1 363 727) according to the instructions given by the manufacturer. The assay has earlier been checked for quantitative reliability (34). The cell pellet (ϳ1 ϫ 10 8 cells) was chilled on ice, washed once with ice-cold 0.1 M Tris (pH 7.8), resuspended in 500 l of lysis buffer (0.4 M MOPS and 1% Triton X-100 (pH 6.5)) supplied with the CAT enzyme-linked immunosorbent immunoassay kit, and then disrupted by vortexing with 0.4 g of glass beads (0.5-mm diameter) for 4 ϫ 30 s with intermediate incubations on ice for at least 1 min.…”
Section: Methodsmentioning
confidence: 99%
“…1 363 727) according to the instructions given by the manufacturer. The assay has earlier been checked for quantitative reliability (34). The cell pellet (ϳ1 ϫ 10 8 cells) was chilled on ice, washed once with ice-cold 0.1 M Tris (pH 7.8), resuspended in 500 l of lysis buffer (0.4 M MOPS and 1% Triton X-100 (pH 6.5)) supplied with the CAT enzyme-linked immunosorbent immunoassay kit, and then disrupted by vortexing with 0.4 g of glass beads (0.5-mm diameter) for 4 ϫ 30 s with intermediate incubations on ice for at least 1 min.…”
Section: Methodsmentioning
confidence: 99%