Quantitative changes in metallothionein expression in target cell-types in the gills of turbot (Scophthalmus maximus) exposed to Cd, Cu, Zn and after a depuration treatment

Alvarado, N.; Quesada, Iban; Hylland, Ketil; Marigómez, Ionan; Soto, Manu

doi:10.1016/j.aquatox.2005.10.017

Cited by 49 publications

(35 citation statements)

References 46 publications

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“…No AMG positive silver grains were observed in gill tissues of tilapias exposed to Hg in this study, although the high Hg level was detected by ICP-AES. The AMG positive grains detected in gill have been reported in Cu-exposed turbot (Scophthalmus maximus; Alvarado et al 2006). Nevertheless, there is no report of AMG staining of the gill of Hg-exposed fish.…”

Section: Discussionmentioning

confidence: 92%

“…Autometallography (AMG) is a potent histochemical staining procedure that is used to localize heavy metals in tissues and cells of fish and other species (Danscher 1984;Danscher et al 2000;Loumbourdis and Danscher 2004;Alvarado et al 2006). Several Hg toxicity studies used AMG to detect small amounts of Hg in the cells of various species (Danscher and Norgaard 1985;Woshner et al 2002;Danscher and Stoltenberg 2006).…”

Section: Introductionmentioning

confidence: 99%

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Short-term Exposure of Nile Tilapia (Oreochromis niloticus) to Mercury

et al. 2012

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To investigate effects of short-term mercury (Hg) exposure in tilapia (Oreochromis niloticus) including histopathological changes, Hg bioaccumulation, and protective role of metallothionein (MT) in different exposure routes, adult tilapias were intraperitoneally injected, orally intubated, or semistatically exposed to 0.5, 1, 2, 5 mg/g mercuric chloride. Histopathology, autometallography (AMG), inductive coupled plasma-atomic emission spectrometry (ICP-AES), and MT immunohistochemistry were determined at 0, 3, 6, 9, 12, and 15 days postexposure. Microscopic lesions were observed in the kidney, hepatopancreas, spleen, and intestine. AMG positive grains were found in renal tubule epithelium, melanomacrophage centers (MMCs), and intestinal epithelium of treated tilapias. Hg concentrations measured by ICP-AES in abdominal visceral organs were significantly higher than in other organs. All exposure routes caused lesions of increasing severity and Hg accumulations in a dose-dependent manner. Semistatic groups produced the highest intensity of lesions, AMG positive staining, as well as total Hg concentrations. Positive MT expression in renal tubule epithelium, pancreatic acini, and splenic MMCs was observed only in semistatic groups. The semistatic exposure route demonstrated the most significant microscopic lesions, Hg bioaccumulation, and MT expression.

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Section: Discussionmentioning

confidence: 92%

Section: Introductionmentioning

confidence: 99%

Short-term Exposure of Nile Tilapia (Oreochromis niloticus) to Mercury

et al. 2012

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“…Taking into account these multiphysiological functions, gills are considered as the dominant uptake site of metals in fish and crustaceans, after direct exposure [39,40]. After direct exposure to acute Pb, Cr, or low methylmercury (CH 3 -Hg) waterborne exposures, accumulation levels in gills were higher than those in HP [2,32,[41][42][43].…”

Section: Feeding Behaviormentioning

confidence: 99%

Relative importance of direct and trophic uranium exposures in the crayfish Orconectes limosus: Implication for predicting uranium bioaccumulation and its associated toxicity

Simon

Floriani

Camilleri

et al. 2012

Enviro Toxic and Chemistry

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Abstract-Pollutants that occur at sublethal concentrations in the environment may lead to chronic exposure in aquatic organisms. If these pollutants bioaccumulate, then organisms higher in the food chain may also be at risk. Increased attention has thus been focused on the relative importance of dietary uptake, but additional knowledge of the cellular distribution of metals after dietary exposure is required to assess the potential toxicity. The authors address concerns relating to increasing uranium (U) concentrations (from 12 mg/L to 2 mg/L) in the freshwater ecosystem caused by anthropogenic activities. The objective of the present study is to compare uranium bioaccumulation levels in tissues and in the subcellular environment. The authors focused on the cytosol fraction and its microlocalization (TEM-EDX) in the gills and the hepatopancreas (HP) of the crayfish Orconectes limosus after 10 d of direct exposure (at concentrations of 20, 100, and 500 mg/L) and five trophic exposure treatments (at concentrations from 1 to 20 mg/g). Results indicated that adsorption of uranium on the cuticle represents the main contribution of total uranium accumulation to the animal. Accumulation in the gills should be considered only as a marker of waterborne uranium exposure. Accumulation in the HP after trophic environmental exposure conditions was higher (18.9 AE 3.8 mg/g) than after direct exposure. Moreover, no significant difference in the subcellular distribution of uranium (50%) in HP was observed between animals that had been exposed to both types of treatment. A potential toxic effect after uranium accumulation could therefore exist after trophic exposure. This confirms the need to focus further studies on the metal (uranium) risk assessment. Environ. Toxicol. Chem. 2013;32:410-416. # 2012 SETAC

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“…Thus they represent the target for the toxic action of metals (Evans, 1987 andOlsson et aL, 1998). However, the variety of cell-types of the gills (chloride cells, mucus cell, pillar cells and undifferentiated cells) makes it difficult to interpret the possible mechanisms of metal accumulation (Alvarado et al, 2006).…”

Section: Gillsmentioning

confidence: 99%

Impact of Certain Heavy Metals on the Gill and Liver of the Nile Tilapia (Oreochromis Niloticus)

Gaber

2007

Egypt. J. of Aquatic Biolo. and Fish.

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T he effect of the heavy metals Cu, Zn, Cd and Pb on the gill and liver tissues of the Nile tilapia, Oreochromis niloticus was examined in the laboratory. One hundred and fifty adult fish were divided into five groups (control, copper, zinc, cadmium and lead) each group containing 30 fish. They were continuously exposed to sub-lethal concentration of each metal (lmg/L) for a period of 10 days. From each group, the gills and liver of six fish were removed after 10 days and the remaining fish were transferred to tap water for : a recovery period (21days). The tissue samples of gills and liver of both treated and recovery fish were dissected for histological examination. The results showed that the extent of distortion of the gills and liver was more pronounced in the fish group that were exposed to Cd&Pb.

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Quantitative changes in metallothionein expression in target cell-types in the gills of turbot (Scophthalmus maximus) exposed to Cd, Cu, Zn and after a depuration treatment

Cited by 49 publications

References 46 publications

Short-term Exposure of Nile Tilapia (Oreochromis niloticus) to Mercury

Short-term Exposure of Nile Tilapia (Oreochromis niloticus) to Mercury

Relative importance of direct and trophic uranium exposures in the crayfish Orconectes limosus: Implication for predicting uranium bioaccumulation and its associated toxicity

Impact of Certain Heavy Metals on the Gill and Liver of the Nile Tilapia (Oreochromis Niloticus)

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