X oilfield is a typical multi-layer sandstone reservoir in offshore China. In the early stage, in order to obtain economic oil production, directional well was used to adopt a set of multi-layer combined production, which resulted in serious interlayer interference, water injection inrush and low reserve utilization. Based on the theory of single-phase unstable seepage flow and the theory of oil-water two-phase non-piston displacement, the author innovatively established a mathematical model of interlayer dynamic interference in multilayer sandstone reservoirs, revealed the influence law of main controlling factors such as permeability, viscosity, starting pressure gradient and reservoir type on interlayer interference, and innovatively formed a quantitative characterization theory of interlayer interference in multilayer combined oil production. The technical demarcation of offshore multi-zone combined oil production reservoir system is formulated and the recombination of oil field development system is guided.