Quantitative Collection and Enzymatic Activity of Glucose Oxidase Nanotubes Fabricated by Templated Layer-by-Layer Assembly

Zhang, Shouwei; Demoustier‐Champagne, Sophie; Jonas, Alain M.

doi:10.1021/acs.biomac.5b00628

Cited by 15 publications

(13 citation statements)

References 58 publications

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“…Moreover, it is a versatile technique which may be applied to almost all substrates independently of their shape, chemical composition and physical properties, including nanoporous materials. 16 Bioactive nanotubes/nanowires have been obtained by some of us [17][18][19] and other groups. [20][21][22] They were successfully used for different purposes, such as the control of preosteoblast adhesion.…”

Section: Introductionmentioning

confidence: 99%

Enzyme-assisted mineralization of calcium phosphate: exploring confinement for the design of highly crystalline nano-objects

et al. 2020

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Section: Introductionmentioning

confidence: 99%

Enzyme-assisted mineralization of calcium phosphate: exploring confinement for the design of highly crystalline nano-objects

et al. 2020

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“…In addition, the enzymatic activity of (bPEI/GOX) 6 collected by the adjuvant-assisted method is preserved, as will be extensively reported elsewhere. 57 As observed in Figure 7a−e, LbL-assembled nanotubes have a tendency to bend, fold, and form loops after being dispersed in aqueous solution. In other words, there is a difference in nanotubes rigidity prior and after being dispersed in aqueous solution.…”

Section: Collection Of Nanotubes By Filtrationmentioning

confidence: 83%

Universal Method to Transfer Membrane-Templated Nano-Objects to Aqueous Solutions

et al. 2015

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A wide range of nano-objects are synthesized by combining template synthesis, using polycarbonate membrane as template, with different material deposition methods. The resulting nanostructures varied from robust inorganic gold nanowires grown by electrodeposition to rigid polypyrrole nanotubes synthesized by chemical polymerization and softer nanotubes made of different combinations of synthetic and natural polyelectrolytes fabricated by layer-by-layer (LbL) assembly. The morphology of these various nano-objects is characterized prior to and after their immersion in water, revealing that the rigidity degree of LbL nanotubes strongly decreases after being in contact with water, leading to highly swollen and flexible nanotubes in aqueous solution that tend to stick to any surface and are very difficult to collect and disperse quantitatively in aqueous solution. Different processes to collect these nano-objects and disperse them in aqueous medium for further analysis and application were then studied. Among them, a method based on simple filtration of nanotubes in the presence of a powdered dextran adjuvant leads to the quantitative collection and dispersion in water of all types of tested cylindrical nano-objects. This universal method to efficiently collect membrane templated nano-objects paves the way to further characterization of a large variety of nanotubes in aqueous solution and to their potential use as cargo nanocarriers or as nanoreactors.

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“…There have been other reports on the preparation of LbL films by electrostatic interaction using QCM [36]. LbL films using DNA or GOx driven by electrostatic interactions have been reported by Zhang et al [37,38] and Kakade et al It is considered that DNA and GOx are both present in the negatively charged layers because DNA and GOx adsorb to positively charged polymers. Figure 4 shows an AFM image and a depth profile of a dried (H-PEI/DNA + GOx) 5 film.…”

Section: Resultsmentioning

confidence: 99%

Decomposition of Glucose-Sensitive Layer-by-Layer Films Using Hemin, DNA, and Glucose Oxidase

et al. 2020

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Glucose-sensitive films were prepared through the layer-by-layer (LbL) deposition of hemin-modified poly(ethyleneimine) (H-PEI) solution and DNA solution (containing glucose oxidase (GOx)). H-PEI/DNA + GOx multilayer films were constructed using electrostatic interactions. The (H-PEI/DNA + GOx)5 film was then partially decomposed by hydrogen peroxide (H2O2). The mechanism for the decomposition of the LbL film was considered to involve more reactive oxygen species (ROS) that were formed by the reaction of hemin and H2O2, which then caused nonspecific DNA cleavage. In addition, GOx present in the LbL films reacts with glucose to generate hydrogen peroxide. Therefore, decomposition of the (H-PEI/DNA + GOx)5 film was observed when the thin film was immersed in a glucose solution. (H-PEI/DNA + GOx)5 films exposed to a glucose solution for periods of 24, 48 72, and 96 h indicated that the decomposition of the film increased with the time to 9.97%, 16.3%, 23.1%, and 30.5%, respectively. The rate of LbL film decomposition increased with the glucose concentration. At pH and ionic strengths close to physiological conditions, it was possible to slowly decompose the LbL film at low glucose concentrations of 1–10 mM.

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Quantitative Collection and Enzymatic Activity of Glucose Oxidase Nanotubes Fabricated by Templated Layer-by-Layer Assembly

Cited by 15 publications

References 58 publications

Enzyme-assisted mineralization of calcium phosphate: exploring confinement for the design of highly crystalline nano-objects

Enzyme-assisted mineralization of calcium phosphate: exploring confinement for the design of highly crystalline nano-objects

Universal Method to Transfer Membrane-Templated Nano-Objects to Aqueous Solutions

Decomposition of Glucose-Sensitive Layer-by-Layer Films Using Hemin, DNA, and Glucose Oxidase

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