Quantitative correlation of morphological alterations of the nucleus with functional events during in vitro infection of glial cells with herpes simplex hominis (HSV 2)

Ultrastructural Localization of the Herpes Simplex Virus Major DNA-binding Protein in the Nucleus of Infected Cells

“…The distribution of immunoperoxidase label in the nuclei of infected cells (Fig. 2a) closely paralleled that found by immunofluorescence, with the majority of electron-dense deposits being restricted to centrally located inclusions known to contain viral material (Darlington & James, 1966;Schwartz & Roizman, 1969;Dupuy-Coin et al, 1978;Puvion-Dutilleul et al, 1982). Occasionally, label was also observed in the peripheral regions of condensed chromatin (Fig.…”

“…2, 5) which are supposed to contain principally condensed host chromatin (Darlington & James, 1966;Schwartz & Roizman, 1969;Dupuy-Coin et al, 1978 ;Puvion-Dutilleul et al, 1982). After mild loosening of nuclear components in the presence of detergent at low ionic strength, these regions no longer reacted with anti-ICSP 1 l, 12 serum, although the extent of labelling in the clear central region remained virtually unchanged (Fig.…”

“…In thin sections, one sees nuclear modifications principally related to the margination of host chromatin, a partial disruption of the nucleoli, the formation of electron-dense 'dense bodies', and the appearance of electron-translucent regions containing virus nucleocapsids in various degrees of completion (Darlington & James, 1966;Schwartz & Roizman, 1969;Dupuy-Coin et al, 1978;Puvion-Dutilleul et al, 1982). In chromatin samples prepared by the spreading technique introduced by Miller & Beatty (1969), the most notable structures are filaments about 10 nm in thickness associated with double-stranded DNA molecules devoid of nucleosomes (Miiller et al, 1980;Puvion-Dutilleul et al, 1982).…”

The Products of Gene US11 of Herpes Simplex Virus Type 1 Are DNA-binding and Localize to the Nucleoli of Infected Cells

“…Studies on the effects of HSV infection on cellular metabolism have led to the conclusion that the nucleolus disaggregates to produce spherical densely staining components (dense bodies) and a less densely staining, irregularly shaped, granular component (Sirtori & Bosisio-Bestetti, 1967;Schwartz & Roizman, 1969;Dupuy-Coin et al, 1978). In contrast to the granular component, the dense bodies contain no DNA or RNA, although they have been shown to contain at least one nucleolus-specific protein (Puvion-Dutilleul et al, 1985).…”

“…7c, d) and were invariably sites of strong localization. These structures are a well recognized feature of HSV-infected cells and constitute one of the residual components of the nucleolus, which disaggregates following HSV infection (Sirtori & Bosisio-Bestetti, 1967;Schwartz & Roizman, 1969;Dupuy-Coin et al, 1978). Their designation as nucleoli was confirmed using differential staining techniques that preferentially stain ribonucleoproteins (Bernhard, 1969; Fig.…”