Quantitative detection of CEA expressing free tumor cells in the peripheral blood of colorectal cancer patients during surgery with real-time RT-PCR on a LightCycler

Ito, Seiji; Nakanishi, Hayao; Hirai, Takashi; Kato, Tomoyuki; Kodera, Yasuhiro; Zhang, Feng; Kasai, Yasushi; Ito, Katsuki; Akiyama, Seiji; Nakao, Akimasa; Tatematsu, Masae

doi:10.1016/s0304-3835(02)00157-x

Cited by 81 publications

(87 citation statements)

References 28 publications

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“…The 14 studies included here comprised 1841 patients in total, with the median number of patients in each study being 99.5 (range 42 -438) (Taniguchi et al, 2000;Yamaguchi et al, 2000;Ito et al, 2002;Bessa et al, 2003;Chen et al, 2004;Sadahiro et al, 2005Sadahiro et al, , 2007Katsumata et al, 2006;Koch et al, 2006;Lloyd et al, 2006;Allen-Mersh et al, 2007;Uen et al, 2007Uen et al, , 2008Wang et al, 2007).…”

Section: Study Characteristicsmentioning

confidence: 99%

“…Of the 14 studies included, eight undertook blood sampling perioperatively (Taniguchi et al, 2000;Yamaguchi et al, 2000;Ito et al, 2002;Chen et al, 2004;Sadahiro et al, 2005;Katsumata et al, 2006;Koch et al, 2006;Lloyd et al, 2006), four undertook sampling approximately 24 h after resection (Bessa et al, 2003;Koch et al, 2006;Lloyd et al, 2006;Allen-Mersh et al, 2007), and six undertook sampling between 24 h and 14 days after resection (Chen et al, 2004; Allen-Mersh et al, 2007;Sadahiro et al, 2007;Uen et al, 2007Uen et al, , 2008Wang et al, 2007).…”

Section: Study Characteristicsmentioning

confidence: 99%

“…Seven investigated the role of CTC in patients with stage-I to stage-III disease undergoing potentially curative surgery (Ito et al, 2002;Bessa et al, 2003;Sadahiro et al, 2005Sadahiro et al, , 2007Allen-Mersh et al, 2007;Wang et al, 2007;Uen et al, 2008); three studied CTC in patients with early CRC (of which two investigated stage-II disease alone (Koch et al, 2006;Uen et al, 2007) and one investigated stage-I and stage-II disease (Lloyd et al, 2006)); and four papers included patients with stage-I to stage-IV disease (Taniguchi et al, 2000;Yamaguchi et al, 2000;Chen et al, 2004;Katsumata et al, 2006).…”

Section: Tumour Stagementioning

confidence: 99%

“…Of the 14 studies included in our analysis, 10 involved sampling of peripheral blood only (Ito et al, 2002;Bessa et al, 2003;Chen et al, 2004;Katsumata et al, 2006;Lloyd et al, 2006;Allen-Mersh et al, 2007;Sadahiro et al, 2007;Uen et al, 2007Uen et al, , 2008Wang et al, 2007); 1 used peripheral and portal blood samples (Sadahiro et al, 2005); 1 used peripheral and mesenteric samples (Yamaguchi et al, 2000); 1 used peripheral, portal and mesenteric samples (Taniguchi et al, 2000); and 1 used samples of central venous blood (Koch et al, 2006).…”

Section: Blood Sampling Sitementioning

confidence: 99%

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Prognostic significance of circulating tumour cells following surgical resection of colorectal cancers: a systematic review

et al. 2010

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Background: The role of adjuvant chemotherapy after resection of colorectal cancers (CRCs) is well understood for patients with stage-I or stage-III disease. Its efficacy for those with stage-II disease remains much less clear. Many investigators have sought to identify prognostic markers that might clarify which patients have the highest risk of recurrence and would, therefore, be most likely to benefit from chemotherapy. This systematic review examines evidence for the use of peripherally sampled, circulating tumour cells (CTCs) as such a prognostic marker. Methods: A comprehensive literature search was used to identify studies reporting on the significance of CTCs in the postoperative blood of CRC patients. Results: Fourteen studies satisfied the inclusion criteria. Six of the nine studies that took blood samples 24 h or more postoperatively found detection of postoperative CTCs to be an independent predictor of cancer recurrence. Conclusion: The presence of CTCs in peripheral blood at least 24 h after resection of CRCs is an independent prognostic marker of recurrence. Further studies are needed to clarify the optimal time point for blood sampling and determine the benefit of chemotherapy in CTC-positive patients with stage-II disease.

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Section: Study Characteristicsmentioning

confidence: 99%

Section: Study Characteristicsmentioning

confidence: 99%

Section: Tumour Stagementioning

confidence: 99%

Section: Blood Sampling Sitementioning

confidence: 99%

See 2 more Smart Citations

Prognostic significance of circulating tumour cells following surgical resection of colorectal cancers: a systematic review

et al. 2010

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“…[17][18][19][20] Although these methods provide valuable information, one of the major limitations is that neither RT-PCR nor Q-PCR is able to analyze more than 1 molecular marker in a single experiment. Several recent RNA-based approaches focus only on clinical significance of single marker analysis.…”

mentioning

confidence: 99%

Development of a high‐throughput membrane‐array method for molecular diagnosis of circulating tumor cells in patients with gastric cancers

Lin

et al. 2006

Intl Journal of Cancer

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Recently several noninvasive methods have been employed to detect circulating tumor cells (CTCs) in cancer patients. In this study, we have developed a highly sensitive, high-throughput colorimetric membrane-array method that was designed to detect a panel of mRNA markers including human telomerase reverse transcriptase (hTERT), , carcinoembryonic antigen (CEA) and mucin 1 (MUC1) mRNA for the presence of CTCs in the peripheral blood of patients with gastric cancer (GC). Digoxigenin-labeled cDNA targets synthesized following total RNA isolation from peripheral blood samples of 64 GC patients and 80 healthy individuals were subjected to membrane-array hybridization. The results showed that membrane array could positively detect 5 cancer cells/ml of peripheral blood in GC cell-dilution experiments. The sensitivity, specificity and diagnostic accuracy for hTERT, CK-19, CEA and MUC1 mRNA ranged from 78.1% to 82.8%, 76.3% to 85% and 81.3% to 83.3%, respectively. Both CEA and MUC1 mRNA expression was correlated significantly with all malignant biological properties of GC, such as macroscopic type, depth of tumor invasion, lymph-node metastasis, TNM stage and coexisting distant metastasis (all p < 0.05). Using these 4 markers in combination, the sensitivity, specificity and diagnostic accuracy of membrane array were raised to 89.1%, 91.3% and 90.3%, respectively. The expression of all 4 mRNA markers was an independent predictor for postoperative recurrence/metastasis. GC patients with the expression of all the 4 mRNA markers showed a poorer survival rate than those without the expression of any 1 mRNA marker (p 5 0.0223). These findings demonstrated that our membrane-array method could detect CTCs in the circulation of GC patients with considerably high sensitivity and specificity. The identification of CTCs in the peripheral blood may be useful in the auxiliary cancer diagnostics or postoperative surveillance of GC patients for recurrence/metastasis. ' 2006 Wiley-Liss, Inc.Key words: circulating tumor cell; membrane array; gastric cancer; molecular diagnosisThe degrees of tumor penetration in the stomach wall and lymph node metastasis have been used for many decades as the 2 major prognostic determinants for gastric cancer (GC) patients. Unfortunately, despite informative staging of patients with GC, some patients with apparently localized disease at diagnosis will subsequently develop recurrent or metastatic diseases. 1-4 One of the major causes is attributed to the presence of disseminated tumor cells shed from the primary carcinoma into circulation, prior to or during surgery. Even in patients with early GC, blood-born metastasis occurs. 2,5,6 Recent attempts to improve staging include sensitive detection of disseminated tumor cells in the blood or lymph nodes by molecular approaches. Evidence increasingly clarifies that primary cancers begin shedding neoplastic cells into the circulation at an early stage. [7][8][9] It is the dissemination of cancer cells into circulation that is widely accepted as the main cause l...

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Advances in Fluorescence‐BasedIn vivoFlow Cytometry for Cancer Applications

Greiner¹,

Georgakoudi²

2011

Advanced Optical Flow Cytometry

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Quantitative detection of CEA expressing free tumor cells in the peripheral blood of colorectal cancer patients during surgery with real-time RT-PCR on a LightCycler

Cited by 81 publications

References 28 publications

Prognostic significance of circulating tumour cells following surgical resection of colorectal cancers: a systematic review

Prognostic significance of circulating tumour cells following surgical resection of colorectal cancers: a systematic review

Development of a high‐throughput membrane‐array method for molecular diagnosis of circulating tumor cells in patients with gastric cancers

Advances in Fluorescence‐BasedIn vivoFlow Cytometry for Cancer Applications

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