Quantitative detection of Clostridium difficile in hospital environmental samples by real-time polymerase chain reaction

Restriction Map For NK104-NK105 of Nonrepeating Region For tcdB Toxin Gene in Clostridium difficile

“…The results of PCR products of 16S rRNA gene amplification revealed the presence of a 157 bp product when compared to the molecular ladder, thus identifying the isolates as C. difficile as shown in fig (1), and this result agrees with Mutters [8]. It has also been suggested that 16S rRNA was chosen for the detection of C. difficile because it exhibits a high degree of functional and evolutionary homology within all bacteria and these sequences have been used for phylogenetic comparisons and classifications of microbial organisms [4].…”

Clostridium difficile infection: Early history, diagnosis and molecular strain typing methods

“…Many studies have developed different real-time PCR (RT-PCR) methods for the detection of C. difficile toxin genes directly from stool samples, not only from humans [166,167] but also from animals [168], and for the quantitative detection of C. difficile in hospital environmental samples [169]. Various automated RT-PCR systems are commercially available, intended as diagnostic tools for CDI.…”

Protective antibody responses againstClostridium difficileelicited by a DNA vaccine expressing the enzymatic domain of toxin B

“…[1][2][3] C. difficile-associated diarrhea is one of the most significant emerging hospital acquired infections in Western countries and its prevalence has increased at an alarming rate over the past two decades. Less developed countries such as China have also seen C. difficile infection in specific hospital patient populations.…”