Quantitative detection of single DNA molecules on DNA tetrahedron decorated substrates

Wang, Zhenguang; Xue, Qingwang; Tian, Wenzhi; Wang, Lei; Jiang, Wei

doi:10.1039/c2cc35208g

Cited by 31 publications

(34 citation statements)

References 26 publications

(26 reference statements)

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“…To evaluate whether these DNA strands could be assembled into one complex, electrophoresis was conducted. As shown in Figure 2A, the four single strands could indeed assemble into the structure of DNA Td as previously described [29]. Moreover, the Apt-tail coupled with the Td and formed a larger DNA structure, indicating that Apt-Td could be constructed following our method.…”

Section: Resultssupporting

confidence: 66%

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Aptamer-guided DNA tetrahedron as a novel targeted drug delivery system for MUC1-expressing breast cancer cells in vitro

Dai

Duan

et al. 2016

Oncotarget

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Mucin 1 (MUC1) is an important molecular target for cancer treatment because it is overexpressed in most adenocarcinomas. In this study, a new MUC1-targeted drug delivery system was assembled using an aptamer (Apt) that could recognize MUC1 and a DNA tetrahedron (Td) that could carry doxorubicin (Dox) within its DNA structure. The complex thus formed (Apt-Td) had an average size of 12.38 nm and was negatively charged. Similar to the MUC1 aptamer, the Apt-Td could preferentially bind with MUC1-positive MCF-7 breast cancer cells. A drug loading experiment revealed that each Apt-Td complex could carry approximately 25 Dox molecules. Moreover, Apt-Td selectively delivered Dox into the MUC1-positive breast cancer cells but reduced Dox uptake by the MUC1-negative control cells. Dox-loaded Apt-Td also induced a significantly higher cytotoxicity to the MUC1-positive cancer cells versus the MUC1-negative control cells in vitro (p<0.01). These results suggest that Apt-Td may potentially serve as a drug carrier in the targeted treatment of MUC1-expressing breast cancers.

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Section: Resultssupporting

confidence: 66%

“…Previous work by Wang et al has clearly demonstrated that the four DNA strands involved in this study can self-assemble into a DNA tetrahedron [29]. Here in this study, the original MUC1 aptamer was extended with an extra tail (Apt-tail) to serve as a sticky end.…”

Section: Resultsmentioning

confidence: 93%

Aptamer-guided DNA tetrahedron as a novel targeted drug delivery system for MUC1-expressing breast cancer cells in vitro

Dai

Duan

et al. 2016

Oncotarget

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“…In yet another approach, electrostatic interactions have been used to encapsulate QDs within a DNA nanocage by Wang et al, to conserve the single molecule aggregation-free state of QDs in solutions [99]. In a similar approach by Wang et al, QD-DNA conjugates have been used for the detection of a correctly 'folded' 3D DNA origami cage [100]. These conjugates are not only novel materials for design and assembly, but also display interesting electronic and chemical properties and therefore are valuable for understanding the macromolecular behaviour of complex nanomaterials.…”

Section: Synthetic Programmable Scaffoldsmentioning

confidence: 99%

Quantum dots–DNA bioconjugates: synthesis to applications

et al. 2016

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Semiconductor nanoparticles particularly quantum dots (QDs) are interesting alternatives to organic fluorophores for a range of applications such as biosensing, imaging and therapeutics. Addition of a programmable scaffold such as DNA to QDs further expands the scope and applicability of these hybrid nanomaterials in biology. In this review, the most important stages of preparation of QD-DNA conjugates for specific applications in biology are discussed. Special emphasis is laid on (i) the most successful strategies to disperse QDs in aqueous media, (ii) the range of different conjugation with detailed discussion about specific merits and demerits in each case, and (iii) typical applications of these conjugates in the context of biology.

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“…Glass‐based fluorescence strategy is often used for quantitative analysis. In one study, DNA tetrahedron with amino groups could be anchored onto a glass slide via epoxy‐amino reaction . Through target‐induced streptavidin‐biotin binding, quantum dots (QDs) were captured as fluorescent probes on DNA nanostructures.…”

Section: Fna‐based Biosensorsmentioning

confidence: 99%

Rational Design of Framework Nucleic Acids for Bioanalytical Applications

Liu

et al. 2019

ChemPlusChem

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With the advent of DNA nanotechnology, nucleic acids have been used building blocks for constructing various DNA nanostructures. As classical and simple DNA nanostructures, framework nucleic acids (FNAs) have attracted enormous attention in the field of biosensing. The sequence‐specific self‐assembly properties and high programmability of nucleic acids allow FNAs to be incorporated in advanced probe design. In addition, FNAs enable the engineering of surfaces for biosensing (e. g., probe orientation, probe spacing), thereby improving the accessibility of target molecules to the probes arranged on heterogeneous surfaces. Moreover, FNAs offer a universal and promising platform for sensing cellular molecules because of their prominent biocompatibility and cellular permeability. Herein recent advances in FNA‐based biosensors are summarized, including electrochemical detection, optical detection, and intracellular sensing. It is hoped that this review will provide guidelines for the design and construction of FNA scaffold‐based biosensors.

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Quantitative detection of single DNA molecules on DNA tetrahedron decorated substrates

Abstract: A single DNA molecule detection method on DNA tetrahedron decorated substrates has been developed. DNA tetrahedra were introduced onto substrates for both preventing nonspecific adsorption and sensitive recognition of single DNA molecules.

Cited by 31 publications

References 26 publications

Aptamer-guided DNA tetrahedron as a novel targeted drug delivery system for MUC1-expressing breast cancer cells in vitro

Aptamer-guided DNA tetrahedron as a novel targeted drug delivery system for MUC1-expressing breast cancer cells in vitro

Quantum dots–DNA bioconjugates: synthesis to applications

Rational Design of Framework Nucleic Acids for Bioanalytical Applications

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