Quantitative determination of arecoline in plasma by gas chromatography chemical ionization mass spectrometry

Abstract

A capillary gas chromatography/mass spectrometry (GC/MS) method for the quantitative analysis of arecoline in plasma has been developed for concentrations in the range 1-50 ng ml-1. Hexadeuterated arecoline was utilized as the internal standard. The removal of drug from plasma was accomplished by a two-step liquid/liquid extraction procedure involving a wash step followed by extraction with 5% triethyl amine in ethyl acetate. The GC/MS determinations were carried out with temperature-programmed capillary GC and ammonia chemical ionization mass spectrometry. The [M + H]+ ions of both analyte and internal standard were monitored at m/z 156 and 162, respectively. The method is linear and has sufficient sensitivity, precision, accuracy and selectivity for analysis of drug levels in human plasma.

PVC Matrix Membrane Sensors for Potentiometric Determination of Arecoline

“…[4][5][6] Therefore, it is important to be able to determine the concentration of arecoline, as this would give an indication of the concentration of arecoline being administered to the keratinocytes and fibroblasts. Previous reports on the determination of arecoline involved, HPLC-UV, [8][9][10][11] HPLC-MS, [12][13][14][15] GC-MS, [16][17][18] capillary electrophoresis (CE), [19] and colorimetry. [16,18] However, some chromatographic methods involve time-consuming procedures, derivatization and=or sophisticated instruments.…”

“…Previous reports on the determination of arecoline involved, HPLC-UV, [8][9][10][11] HPLC-MS, [12][13][14][15] GC-MS, [16][17][18] capillary electrophoresis (CE), [19] and colorimetry. [16,18] However, some chromatographic methods involve time-consuming procedures, derivatization and=or sophisticated instruments. Recently, potentiometric membrane sensors have been more extensively used in different matrices.…”

Quantification of arecoline (areca nut alkaloid) in neonatal biological matrices by high‐performance liquid chromatography/electrospray quadrupole mass spectrometry

“…To our knowledge, the only analytical method reported for arecoline in biological matrices is an assay based on gas chromatography coupled with mass spectrometry. This method, with a limit of quantification of 1 ng/mL, was developed16 for the detection of arecoline in plasma of volunteers who participated in experiments on the application of prototype transdermal systems. In addition, an extensive review on poisoning by plant material proposed an HPLC/MS technique for the detection of arecoline, but with no reported application to real cases 17.…”