Quantitative Determination of Compounds from Akebia quinata by High-Performance Liquid Chromatography

Abstract

To provide the scientific corroboration of the traditional uses of Akebia quinata (Thunb.) Decne., a detailed analytical examination of A. quinata stems was carried out using a reversed-phase high performance liquid chromatography (RP-HPLC) method coupled to photodiode array detector (PDA) for the simultaneous determination of four phenolic substances; cuneataside D (1), 2-(3,4-dihydroxyphenyl)ethyl-O-β-D-glucopyranoside (2), 3-caffeoylquinic acid (3) and calceolarioside B (4). Particular attention was focused on the main compound, 3-caffeoylquinic acid (3), which has a range of biological functions. In addition, 2-(3,4-dihydroxyphenyl)ethyl-O-β-D-glucopyranoside (2) was considered as a discernible marker of A. quinata from its easy confuse plants. The contents of compounds 2 and 3 ranged from 0.72 to 2.68 mg/g and from 1.66 to 5.64 mg/g, respectively. The validation data indicated that this HPLC/PDA assay was used successfully to quantify the four phenolic compounds in A. quinata from different locations using relatively simple conditions and procedures. The pattern-recognition analysis data from 53 samples classified them into two groups, allowing discrimination between A. quinata and comparable herbs. The results suggest that the established HPLC/PDA method is suitable for quantitation and pattern-recognition analyses for a quality evaluation of this medicinal herb.

Polyphenolic compounds as electron shuttles for sustainable energy utilization

“…Quinones and derivatives Hydroxyechinofuran BHydroxyechinofuran B (15 mg) from 1 g of fresh hairy roots of Lithospermum erythrorbizon [89] 6-(4,7-Hydroxy-heptyl) cyclohex-2,5-diene-ene-1,4-dione (6-(4,7-hydroxy-heptyl) quinone)the new quinone isolated from ten grams of crude ethyl acetate extract of 500 g Pergularia daemia leaves[90] Scabequinone~ 7 × 10 −3 % from fresh Cyperus distans weight[91] 2,6-Dimethoxyquinone~ 0.002% based on root of Rauwolfia vomitoria [92]1.1% of aqueous methanol fraction from 15 g dried stems of Tibouchina pulchra [93] 2,5-Dihydroxy-3-undecyl-para-benzoquinone (Embelin)1.9 g embelin from 100 g of Embelia ribes extracted using hexane[94]B-2. Catechol and derivatives Catechol (1,2-dihydroxybenzene).0.1–0.2 g of crude catechol from 100 g of dry scales of pigmented onion scales ( Allium cepa )[95] Protocatechuic acid (3,4-dihydroxybenzoic acid)~ 0.1 g/100 g dry scales of pigmented onion scales ( Allium cepa )[96] Carnosic acid3.77 % in Salvia officinalis L.[56]7.57% in Thymus vulgaris L.[56] Dopaol beta- d -glucoside (2-(3,4-dihydroxyphenyl) ethyl β- d -gluco-pyranoside)~ 3 mg/g dry sample of Akebia quinata [57] Horminone0.4 g from about 1 kg of the dried whole plant (aerial parts and roots of Salvia lanata )[97]B-3. Naphthoquinones and derivatives 3′3-Biplumbagin~ 10% from the n-hexane extracts of stem bark (20 g) of Diospyros anisandra [98] Diosquinone120 μg from 5 kg of the dried root of Diospyros mespiliformis […”

Phenolic Profile and Fingerprint Analysis of Akebia quinata Leaves Extract with Endothelial Protective Activity

“…The simultaneous determination of multiple bioactive components has been a recent developing trend in evaluating the quality of natural products; however, no effective HPLC method has been reported to comprehensively evaluate the quality of A. quinata leaves. Some published analytical methods that only control the quality of the fruits and stems of A. quinata include HPLC-ultraviolet (HPLC–UV) detection method and HPLC coupled with electrospray ionization-quadrupole-time-of-flight mass spectrometry (HPLC–ESI–Q-TOF–MS-MS) [ 20 , 21 , 22 ]. Unfortunately, none of these established methods can be used to identify and quantify the active ingredients in A. quinata leaves because the distribution of the individual metabolites varies greatly in the different parts of A. quinata .…”

Quantitative and Pattern Recognition Analyses of Five Marker Compounds in Raphani Semen using High‐Performance Liquid Chromatography

“…High‐performance liquid chromatography (HPLC) analysis/pattern recognition with multivariate statistical analysis can provide information about the chemical composition, including the marker compounds traditionally used for quality control. For this reason, several researchers have recently developed HPLC and pattern recognition analytical methods for the purpose of quality control and to determine the origin of certain herbal medicines . In our continued efforts to contribute to the chemical investigation of pharmaceutical plants, Raphani Semen (RS) was selected for further study.…”