Quantitative determination of ginsenosides from Panax ginseng roots and ginseng preparations by thin layer chromatography–densitometry

Corthout, J.; Naessens, Tania; Apers, Sandra; Vlietinck, A. J.

doi:10.1016/s0731-7085(99)00109-0

Cited by 56 publications

(35 citation statements)

References 6 publications

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“…Chung et al (2012) reported the absence of ginsenoside Rc from the roots of the P. ginseng variety "Jankyung" growing in three locations in Korea. These results are also in contrast to the observations of Shi et al (2007) and Corthourt et al (1999). These differences might be due to a multiplicity of factors related to genetic or enviromental determinants (temperature, soil conditions, light intensity, root microfl ora), the ginseng cultivar, and processing conditions (Chung et al, 2012;Du et al, 2004).…”

Section: Resultscontrasting

confidence: 63%

Field Cultivation and in vitro Cultures, Root-Forming Callus Cultures and Adventitious Root Cultures, of Panax quinquefolium as a Source of Ginsenosides

Szymańska

Kochan

Szymczyk

2013

Zeitschrift Für Naturforschung C

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The content of six ginsenosides (Rb1, Rb2, Rc, Rd, Rg1, and Re) was studied in the roots of fi eld-grown plants, as well as in root-forming callus cultures and adventitious root cultures of Panax quinquefolium using high-performance liquid chromatography (HPLC). The highest level of saponins was isolated from root hairs (128 mg/g dry weight). The examined in vitro culture synthesized all identifi ed saponins, although in smaller amounts than those obtained from fi eld cultivation. Metabolites Rb1 and Re dominated in the ginseng biomass from both fi eld crops and in vitro culture.

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Section: Resultscontrasting

confidence: 63%

Field Cultivation and in vitro Cultures, Root-Forming Callus Cultures and Adventitious Root Cultures, of Panax quinquefolium as a Source of Ginsenosides

Szymańska

Kochan

Szymczyk

2013

Zeitschrift Für Naturforschung C

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“…It has been generally agreed, that R b :R g ratio is an indicator of raw material quality. In comparison with Asiatic species (P. ginseng), American ginseng contains more panaxosides from R b group and their malonyl derivatives (Corthout et al 1999;Wang et al 1999). Environmental conditions affecting the growth of plants may also influence ginsenosides synthesis and accumulation (Reynolds 1998;Reeleder et al 2000).…”

Section: Resultsmentioning

confidence: 99%

The Effect of Growth Regulators on Quality Parameters and Ginsenosides Accumulation in Panax quinquefolium L. Roots

Kołodziej

Kochan²,

Jerzy³

et al. 2006

Plant Growth Regul

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American ginseng (Panax quinquefolium L.) is a perennial medicinal herb originally grown in Canada and USA, and recently also in China, Australia, Holland and Poland. Several commercial preparations are produced from ginseng roots, that are known for their antifatigue, antitumor, antistress and immune system stimulating functions. The medicinal properties are due mainly to the active components -ginsenosides. In this work, the results of field cultivation experiments are presented that examine the effects of foliar application of several growth regulators on quality parameters and ginsenoside content of P. quinuefolium roots. The growth regulators tested, i.e., kinetin, daminozide, mixture of gibberellic acid (GA 3 ) with potassium salt of a-naphthalene acetic acid (kNAA) and new preparation -IPO-1 -benzimidazole derivative (obtained from the Institute of Organic Industry in Warsaw -at present during the process of patent), were applied at a concentration of 100 or 200 mg l À1 in the middle of June in the 2nd year of vegetation. After 4 years of cultivation, the roots were dug up and dried, and subsequently the quantitative analysis of individual saponins (R b1 , R b2 , R c , R d , R e , R g1 ) by HPLC was performed. Growth regulators significantly affected quality parameters, morphological features and accumulation of individual and total ginsenosides in ginseng roots. Regardless of doses, the plant roots treated with growth regulators had a higher content of total ginsenosides in comparison to the control. The growth regulators also affected individual ginsenosides level and narrowed the ratio of R b :R g group. The application of kinetin, daminozide and benzimidazole derivative for foliar spray during 2nd year of American ginseng vegetation caused a significant increase in air dry weight of roots and aboveground parts whereas the mixture of GA 3 and kNAA showed a decreasing effect. An increase of roots size was observed using higher doses (200 mg l À1 ) of kinetin and daminozide while a decreasing tendency appeared with the application of the other preparations.

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“…PD 그룹은 Rb1, Rb2, Rc, Rd, Rb3 등의 화합물을 PT 그룹은 Re, Rf, Rg1, Rh1 등의 화합물을 가진다 [5,9,20,25,26]. Ginsenodise는 또한 극성(Rg1, Re, Rb1, Rc, Rb, Rd) 및 비극성(Rg6, F4, Rk3, Rh4, Rk1, Rg5) 부분을 가지는 화합물들로 나누어 지는데, 이 러한 성질들의 화합물을 분석 및 정량화하기 위하여 gas chromatography [2], thin-layer chromatography [8] …”

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Simultaneous Analysis Method for Polar and Non-polar Ginsenosides in Cultivated Wild Ginseng by Reversed-phase HPLC-CAD

옥선¹,

강재선²,

김강민³

2016

Journal of Life Science

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Cultivated wild ginseng is a widely used dietary supplement and medicinal herb. The aim of this study was to optimize the ginseng using high performance liquid chromatography (HPLC)-charged aerosol detection (CAD) for ginsenoside analysis. CAD measures the physical property of an analyte and responds to almost all non-volatile species, independent of their nature, spectral properties, or particle size. It has become widely employed in pharmaceutical analysis. The cultivated wild ginseng extracts were analyzed for compositions of ginsenosides Rb1, Rd, Rg1, Rf, Re, and Rh1. The optimal analysis condition was set up from an experiment using a gradient. Ten grams of cultivated wild ginseng were extracted with 95% EtOH 100 ml for 24 hr at 80 o C. The contents of the 6six major ginsenosides in the cultivated wild ginseng extract were Rb1 (5.48±0.12 mg/g), Rd (5.33±0.14 mg/g), Rg1 (12.80± 0.05 mg/g), Rf (19.08±0.68 mg/g), Re (19.87±0.05 mg/g), and Rh1 (16.47±0.16 mg/g), respectively. HPLC showed that the protopanaxatriol group (Rg1, Rf, Re, Rh1) had more content than the protopanaxadiol group (Rb1, Rd) in cultivated wild ginseng extract. In summary, the ginsenosides were identified with HPLC-CAD analysis, and their presence and quantity imply the importance of quality control, as well as the pharmacological activity of the ginseng root.

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Quantitative determination of ginsenosides from Panax ginseng roots and ginseng preparations by thin layer chromatography–densitometry

Cited by 56 publications

References 6 publications

Field Cultivation and in vitro Cultures, Root-Forming Callus Cultures and Adventitious Root Cultures, of Panax quinquefolium as a Source of Ginsenosides

Field Cultivation and in vitro Cultures, Root-Forming Callus Cultures and Adventitious Root Cultures, of Panax quinquefolium as a Source of Ginsenosides

The Effect of Growth Regulators on Quality Parameters and Ginsenosides Accumulation in Panax quinquefolium L. Roots

Simultaneous Analysis Method for Polar and Non-polar Ginsenosides in Cultivated Wild Ginseng by Reversed-phase HPLC-CAD

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