Quantitative determination of guggulsterone in existing natural populations of Commiphora wightii (Arn.) Bhandari for identification of germplasm having higher guggulsterone content

Kulhari, Alpana; Sheorayan, Arun; Chaudhury, Ashok; Sarkar, Susheel Kumar; Kalia, Rajwant K.

doi:10.1007/s12298-014-0271-1

Cited by 11 publications

(7 citation statements)

References 23 publications

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“…[23] Concentration of bio active component are influenced by many factors like Season, collection area , annual rain fall, geographical variation, Planting, harvesting practices, solvent system, mobile phase etc. [24] Impact of reduction of these sterones after Śodhana is not justifiable here and needs more data from experimental and clinical trials.…”

Section: Resultsmentioning

confidence: 99%

Pharmaceutical Standardization of Guggulu Śodhana

Vyas¹,

Shukla²,

Galib³

et al. 2017

JAMS

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Introduction: Śodhana is a preliminary process that helps in eliminating possible physical or chemical blemishes from raw material and making them suitable for therapeutic application. Guggulu (Commiphora wightii (Arn) Bhandari) is a well-known herb in Āyurveda, which is gaining its attention in world market. Exudate of the plant is therapeutically active and need to process before it is converted into formulations. As a little data is available on standard manufacturing procedure of Guggulu Śodhana; the current attempt is planned to evaluate the same. Methods: Guggulu was procured from Gujarat state forest department corp. Ltd., Vadodara. Śodhana was done in 10 batches in Gomūtra. Organoleptic characters, loss on drying, ash value, acid insoluble ash, water soluble extractives, methanol soluble extractives, pH and volatile oil content, HPTLC, HPLC, FTIR, Heavy metal analysis and Microbial over load were carried out. Results: Average 80.56% yield was observed after Śodhana. Differences were found in physico-chemical, HPLC and FTIR analysis that show the impact of Śodhana procedure on Guggulu. Conclusion: The adopted method for Guggulu Śodhana can be considered as easy, convenient and standard procedure and the values of physicochemical parameters may be taken for quality assurance.

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Section: Resultsmentioning

confidence: 99%

Pharmaceutical Standardization of Guggulu Śodhana

Vyas¹,

Shukla²,

Galib³

et al. 2017

JAMS

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“…Mean detected values of guggulsterone-E and guggulsterone-Z were 730.28 and 710.84 μg/g in UHPLC, and 692.97 and 699.87 μg/g in HPTLC, respectively, showing prominence of these two core ingredients of MYG. These cis and trans isomers (guggulsterone-E and guggulsterone-Z) have been estimated by many researchers in raw material (gum-resin, stem, leaf, and root) of Commiphora wightii [52,53] and its commercial formulations [54]. This is worth mentioning that simultaneous, rapid determination of guggulsterones and other signature components of MYG has still been underexplored.…”

Section: Comparative Quantitative Analysis Of Myg Using Uhplc and Hptlcmentioning

confidence: 99%

Standardization and validation of phytometabolites by UHPLC and high‐performance thin layer chromatography for rapid quality assessment of ancient ayurvedic medicine, Mahayograj Guggul

Balkrishna

Tomer

Joshi

et al. 2022

J of Separation Science

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MahayograjGuggul is an ancient ayurvedic medicine, prescribed for various joint disorders like arthritis, gout, and rheumatism. The present research was envisaged to develop a simple, sensitive, and comprehensive analytical method for standardization of Mahayograj Guggul. The analysis was conducted for gallic acid, protocatechuic acid, vanillic acid, cinnamic acid, piperine, guggulsterone-E, and guggulsterone-Z by high-performance thin-layer chromatography, and additionally ferulic acid, ellagic acid, and picroside I by ultra high-performance liquid chromatography. These developed methods were validated as per international guidelines, and were found linear (r 2 > 0.99), sensitive, precise (relative standard deviation < 5%), and accurate with recovery values (85-105%). The limit of detection and quantification were in the range of 0.11-23.6 and 0.33-71.51 μg/g. Gas chromatography tandem mass spectrometry was used to develop Mahayograj Guggul fingerprinting profile and to identify mid-polar or nonpolar compounds. Proximate analysis was used to ascertain the functional groups present in Mahayograj Guggul. Ultra high-performance liquid chromatography and gas chromatography tandem mass spectrometry were further employed to authenticate quality reproducibility in the active ingredients of Mahayograj Guggul in six commercial batches. Taken together, these analytical methods provide a scientific basis and reference for quality control evaluation of Mahayograj Guggul and similar traditional broad-spectrum formulations.

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“…These E- and Z-GS are steroidal isomers and are inter-convertible in 3D space. They differ in the arrangement of CH3 molecule at C20 position and a distorted rotation of C-C double bond present at C17 and C20 positions is observed [ 104 ].…”

Section: Chemical Nature Of Gsmentioning

confidence: 99%

Potential of guggulsterone, a farnesoid X receptor antagonist, in the prevention and treatment of cancer

Girisa

Dey

Harsha

et al. 2020

Exploration of Targeted Anti-Tumor Therapy

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Cancer is one of the most dreadful diseases in the world with a mortality of 9.6 million annually. Despite the advances in diagnosis and treatment during the last couple of decades, it still remains a serious concern due to the limitations associated with currently available cancer management strategies. Therefore, alternative strategies are highly required to overcome these glitches. The importance of medicinal plants as primary healthcare has been well-known from time immemorial against various human diseases, including cancer. Commiphora wightii that belongs to Burseraceae family is one such plant which has been used to cure various ailments in traditional systems of medicine. This plant has diverse pharmacological properties such as antioxidant, antibacterial, antimutagenic, and antitumor which mostly owes to the presence of its active compound guggulsterone (GS) that exists in the form of Z- and E-isomers. Mounting evidence suggests that this compound has promising anticancer activities and was shown to suppress several cancer signaling pathways such as NF-κB/ERK/MAPK/AKT/STAT and modulate the expression of numerous signaling molecules such as the farnesoid X receptor, cyclin D1, survivin, caspases, HIF-1α, MMP-9, EMT proteins, tumor suppressor proteins, angiogenic proteins, and apoptotic proteins. The current review is an attempt to summarize the biological activities and diverse anticancer activities (both in vitro and in vivo) of the compound GS and its derivatives, along with its associated mechanism against various cancers.

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Quantitative determination of guggulsterone in existing natural populations of Commiphora wightii (Arn.) Bhandari for identification of germplasm having higher guggulsterone content

Cited by 11 publications

References 23 publications

Pharmaceutical Standardization of Guggulu Śodhana

Pharmaceutical Standardization of Guggulu Śodhana

Standardization and validation of phytometabolites by UHPLC and high‐performance thin layer chromatography for rapid quality assessment of ancient ayurvedic medicine, Mahayograj Guggul

Potential of guggulsterone, a farnesoid X receptor antagonist, in the prevention and treatment of cancer

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