Quantitative Differences in Antibiotic Resistance Between Methicillin-Resistant and Methicillin-SusceptibleStaphylococcus aureusStrains Isolated in Hungary, Austria and Macedonia

Horváth, Andrea; Rozgonyi, Ferenc; Pesti, N.; Kocsis, Erika; Malmos, G.; Kristóf, Katalin; Nagy, K.; Lagler, Heimo; Presterl, Elisabeth; Stich, Karin; Gattringer, Rainer; Kotolàcsi, G.; Cekovska, Zaklina; Graninger, Wolfgang

doi:10.1179/joc.2010.22.4.246

Cited by 3 publications

(2 citation statements)

References 16 publications

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“…Despite of this, the largest MICs were always exhibited by the same strains, suggesting a differential resistance as compared to more susceptible ones. This resistance level, however, was much lower than that recorded in clinically-resistant S. aureus strains (16-256 µg mL -1 ) [54,55]. Low resistance might be provided by dissimilar cell structures (cell-wall permeability, membrane charges, penicillin binding protein variants, etc.)…”

Section: Discussionmentioning

confidence: 70%

Antibiotic Resistance/Susceptibility Profiles of Staphylococcus equorum Strains from Cheese, and Genome Analysis for Antibiotic Resistance Genes

Vázquez¹,

Srednik²,

Rodrı́guez³

et al. 2023

Preprint

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In this work, the resistance/susceptibility (R/S) profile of Staphylococcus equorum strains (n=30) from cheese to 16 antibiotics was determined by broth microdilution. The minimum inhibitory concentration (MIC) for all antibiotics was low in most strains, although higher MICs compatible with acquired genes were also noted. Genome analysis of 13 strains showed the S. equorum resistome to be composed of intrinsic mechanisms, acquired mutations, and acquired genes. However, the genetic data did not always correlate with the phenotype. As such, a cat gene providing resistance to chloramphenicol was found on a plasmid in one strain; this was able to provide resistance to Staphylococcus aureus after electroporation. An msr(A) polymorphic gene was identified in five strains. The Mrs(A) variants were associated with variable resistance to erythromycin. All strains harboured a polymorphic fosB/fosD gene, although only one acquired copy was associated with strong resistance to fosfomycin. Similarly, a plasmid-associated blaR1-blaZI operon encoding a penicillinase was identified in five ampicillin- and penicillin G-susceptible strains. Identified genes not associated with resistance further included mph(C) in two strains and norA in all strains. The antibiotic R/S status and gene content of S. equorum strains intended to be employed in food systems should be carefully determined.

show abstract

Section: Discussionmentioning

confidence: 70%

Antibiotic Resistance/Susceptibility Profiles of Staphylococcus equorum Strains from Cheese, and Genome Analysis for Antibiotic Resistance Genes

Vázquez¹,

Srednik²,

Rodrı́guez³

et al. 2023

Preprint

View full text Add to dashboard Cite

show abstract

“…However, the largest MICs were always exhibited by the same strains, suggesting a differential resistance as compared to more susceptible ones. This resistance level, however, was much lower than that recorded in clinically resistant S. aureus strains (16-256 µg mL −1 ) [40]. Low resistance might be provided by dissimilar cell structures (cell-wall permeability, membrane charges, penicillin-binding protein variants, etc.)…”

Section: Phenotypic Testing and Proposal Of S Equorum-specific Cut-offsmentioning

confidence: 66%

Antibiotic Resistance/Susceptibility Profiles of Staphylococcus equorum Strains from Cheese, and Genome Analysis for Antibiotic Resistance Genes

Vázquez

Srednik

Rodrı́guez

et al. 2023

IJMS

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In food, bacteria carrying antibiotic resistance genes could play a prominent role in the spread of resistance. Staphylococcus equorum populations can become large in a number of fermented foods, yet the antibiotic resistance properties of this species have been little studied. In this work, the resistance/susceptibility (R/S) profile of S. equorum strains (n = 30) from cheese to 16 antibiotics was determined by broth microdilution. The minimum inhibitory concentration (MIC) for all antibiotics was low in most strains, although higher MICs compatible with acquired genes were also noted. Genome analysis of 13 strains showed the S. equorum resistome to be composed of intrinsic mechanisms, acquired mutations, and acquired genes. As such, a plasmidic cat gene providing resistance to chloramphenicol was found in one strain; this was able to provide resistance to Staphylococcus aureus after electroporation. An msr(A) polymorphic gene was identified in five strains. The Mrs(A) variants were associated with variable resistance to erythromycin. However, the genetic data did not always correlate with the phenotype. As such, all strains harbored a polymorphic fosB/fosD gene, although only one acquired copy was associated with strong resistance to fosfomycin. Similarly, a plasmid-associated blaR1-blaZI operon encoding a penicillinase system was identified in five ampicillin- and penicillin G-susceptible strains. Identified genes not associated with phenotypic resistance further included mph(C) in two strains and norA in all strains. The antibiotic R/S status and gene content of S. equorum strains intended to be employed in food systems should be carefully determined.

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In Vitro Antibiotic Resistance in Bacterial Infected Eczema at Ho Chi Minh City Hospital of Dermatology

Ngoc¹,

Vu²,

Van³

et al. 2019

Open Access Maced J Med Sci

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BACKGROUND:Infected eczema is one of the most common complications of eczema. The progression and treatment of infected eczema have become more complex and difficulty due to the antibiotic resistance of bacteria and the abuse of antibiotics in treatment.AIM:Our research was conducted with the aim of investigating the severity of in vitro antibiotic resistance in patients with bacterially infected eczema at Ho Chi Minh City Hospital of Dermatology.METHODS:We studied 40 cases of patients, suffering from atopic dermatitis, contact dermatitis, vesicular palmoplantar eczema, with positive results of infected eczema.RESULTS:S. aureus accounted for 82.5%, followed by S. epidermidis (15%), P. aeruginosa (12.5%), S. pyogenes (5%) accounted for a small percentage. E. coli (2.5%) and M. morganii (2.5%) accounted for the lowest percentage. Both MSSA and MRSA were completely resistant to penicillin. MRSA is completely resistant to penicillin, erythromycin, and cefuroxime, highly resistant to clindamycin (82.35%). Our research showed that Pseudomonas aeruginosa was not resistant to a variety of antibiotics. It was completely resistant to tetracycline, trimethoprim/sulfamethoxazole (100%). Most bacteria are highly sensitive to linezolid, vancomycin as other studies in the world shown. There are also rifampicins, pristinamycin. Hence, it`s prioritised to be used for only patients with eczema infected with multidrug-resistant bacteria.CONCLUSION:Penicillin is not recommended for the treatment for infected eczema. Linezolid, vancomycin has a high sensitivity to bacteria including multidrug-resistant bacteria like MRSA.

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Quantitative Differences in Antibiotic Resistance Between Methicillin-Resistant and Methicillin-SusceptibleStaphylococcus aureusStrains Isolated in Hungary, Austria and Macedonia

Cited by 3 publications

References 16 publications

Antibiotic Resistance/Susceptibility Profiles of <em>Staphylococcus equorum </em>Strains from Cheese, and Genome Analysis for Antibiotic Resistance Genes

Antibiotic Resistance/Susceptibility Profiles of <em>Staphylococcus equorum </em>Strains from Cheese, and Genome Analysis for Antibiotic Resistance Genes

Antibiotic Resistance/Susceptibility Profiles of Staphylococcus equorum Strains from Cheese, and Genome Analysis for Antibiotic Resistance Genes

In Vitro Antibiotic Resistance in Bacterial Infected Eczema at Ho Chi Minh City Hospital of Dermatology

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