Quantitative Electron Microscopy in the Diagnosis of Mycosis Fungoides

Payne, Claire M.; Nagle, Raymond B.; Lynch, Peter

doi:10.1001/archderm.1984.01650370069013

Cited by 26 publications

(2 citation statements)

References 34 publications

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“…To improve the reproducibility and sensitivity of the diagnosis of MF, key histological features have been identified 5–8 and several adjunctive tests developed through the years. To define the malignant infiltrate, various tests have been employed: abnormal nuclear DNA content and chromosome complement, 9–12 abnormal nuclear contour and shape, 13–17 loss of pan T‐cell markers, 18,19 elevated CD4/CD8 ratio (≥ 6), 20 diffuse epidermal HLA‐DR expression, 21 and the presence of a clonal T‐cell population as measured by T‐cell receptor gene rearrangement (TCR‐GR) studies 22–25 . None of these tests alone firmly establishes the diagnosis of MF.…”

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confidence: 99%

A simple clinical scoring system to improve the sensitivity and standardization of the diagnosis of mycosis fungoides type cutaneous T-cell lymphoma: logistic regression of clinical and laboratory data

Stevens

Bırol

et al. 2003

Br J Dermatol

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confidence: 99%

A simple clinical scoring system to improve the sensitivity and standardization of the diagnosis of mycosis fungoides type cutaneous T-cell lymphoma: logistic regression of clinical and laboratory data

Stevens

Bırol

et al. 2003

Br J Dermatol

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“…However, quantitative electron microscopy of lymphocyte nuclear parameters appears to be a more reliable diagnostic too1. 78,79 Flow cytometry and an assessment of single-cell DNA content have also been shown to facilitate the early diagnosis of M F by the demonstration of aneuploidy. 80 Wantzin et a1.…”

Section: The Diagnosis Of Early Mf Andpremycotic Lesionsmentioning

confidence: 99%

Recent developments in cutaneous lymphoroliferative disorders

Slater

1987

The Journal of Pathology

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An Ultrastructural Morphometric and Immunohistochemical Analysis of Cutaneous Lymphomas and Benign Lymphocytic Infiltrates of Skin

Payne

Grogan

Lynch³

1986

Arch Dermatol

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Our combined ultrastructural, immunohistochemical, histologic, and clinical studies over the past five years have allowed us to compile diagnostic criteria useful in the evaluation of cutaneous lymphomas. As a group, mycosis fungoides (MF) patients could be distinguished from those with benign disorders of skin using ultrastructural morphometry (mean form factor and perimeter values), but with some overlap between groups. Another approach, the ultrastructural histogram method, however, clearly separated MF patients from patients with chronic dermatitis. Immunohistochemistry was useful in distinguishing cases of cutaneous peripheral T-cell lymphoma from MF cases on the basis of the occurrence of "novel phenotypes." Neoplastic T-cell infiltrates of skin can usually be distinguished from benign polyclonal T-cell infiltrates by the presence of one T-cell subset to the exclusion of others. Patients with convoluted B-cell lymphomas could also be distinguished from MF patients using ultrastructural morphometric dual parameter analysis. The diagnostic complexity of several cutaneous T-cell lymphoma cases is illustrated. We have emphasized in this study the strength of combining quantitative electron microscopy, immunohistochemistry, and histology in the diagnostic workup of cutaneous lymphomas. This integrative approach may be necessary to assure a definitive diagnosis in difficult cases.

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Quantitative Electron Microscopy in the Diagnosis of Mycosis Fungoides

Cited by 26 publications

References 34 publications

A simple clinical scoring system to improve the sensitivity and standardization of the diagnosis of mycosis fungoides type cutaneous T-cell lymphoma: logistic regression of clinical and laboratory data

A simple clinical scoring system to improve the sensitivity and standardization of the diagnosis of mycosis fungoides type cutaneous T-cell lymphoma: logistic regression of clinical and laboratory data

Recent developments in cutaneous lymphoroliferative disorders

An Ultrastructural Morphometric and Immunohistochemical Analysis of Cutaneous Lymphomas and Benign Lymphocytic Infiltrates of Skin

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