Quantitative Estimation and Validation of Metformin Hydrochloride and Gliclazide in their Tablet Dosage Form by RP-HPLC

Shinde, Ganesh S.; K, Godge Rahul; Jadhav, R. K.

doi:10.5958/2349-2988.2019.00030.5

Cited by 6 publications

(1 citation statement)

References 0 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Different methods for estimating GLZ in dosage forms have been reported. These methods include high performance liquid chromatography (HPLC) [5][6][7], spectrophotometry [2,[8][9][10][11][12] and spectrofluorimetry [13][14][15]. Similarly, SXG was determined using HPLC [16][17][18], spectrophotometric [19][20][21][22][23][24] and spectrofluourimetric [20,22,25] methods.…”

Section: Introductionmentioning

confidence: 99%

Application of sulfur and nitrogen doped carbon quantum dots as sensitive fluorescent nanosensors for the determination of saxagliptin and gliclazide

et al. 2022

View full text Add to dashboard Cite

In this study, highly fluorescent sulfur and nitrogen doped carbon quantum dots (S,N-CQDs) were used as fluorescent nanosensors for direct spectrofluorimetric estimation of each of gliclazide (GLZ) and saxagliptin (SXG) without any pre-derivatization steps for the first time. S,N-CQDs were synthesized employing a simple hydrothermal technique using citric acid and thiosemicarbazide. The produced S,N-CQDs were characterized using different techniques including fluorescence emission spectroscopy, UV spectrophotometry, high-resolution transmission electron microscopy and FT-IR spectroscopy. Following excitation at 360 nm, S,N-CQDs exhibited a strong emission peak at 430 nm. The native fluorescence of S,N-CQDs was quantitatively enhanced by addition of increased concentrations of the studied drugs. The fluorescence enhancement of S,N-CQDs and the concentrations of the studied drugs revealed a wide linear relationship in the range of 30.0–500.0 µM and 75.0–600.0 µM with limits of detection of 5.0 and 10.15 µM for GLZ and SXG, respectively. The proposed method was efficiently used for determination of cited drugs in their commercial tablets with % recoveries ranging from 98.6% to 101.2% and low % relative standard deviation values (less than 2%). The mechanism of interaction between S,N-CQDs and the two drugs was studied. Validation of the proposed method was carried out in accordance with International Conference on Harmonization (ICH) guidelines.

show abstract

Section: Introductionmentioning

confidence: 99%

Application of sulfur and nitrogen doped carbon quantum dots as sensitive fluorescent nanosensors for the determination of saxagliptin and gliclazide

et al. 2022

View full text Add to dashboard Cite

show abstract

Stability Indicating HPLC Method Development and Validation for Simultaneous Estimation of Metformin and Empagliflozin in Bulk and Pharmaceutical Dosage Form

Pathan

Kshirsagar²

2022

RJPT

View full text Add to dashboard Cite

In the present work, simple, accurate and rapid stability indicating RP-HPLC method has been developed for the simultaneous estimation of Metformin and Empagliflozin in bulk and tablet dosage form. Separation of analytes was carried out on Ascentis C18 HPLC column (4.6 x 150 mm, 5 μm) using a mobile phase of 0.1% orthophosphoric acid and acetonitrile (60:40, v/v), at a flow rate of 1.0mL/min and measurement carried out at UV 260 nm. The validation parameters namely linearity, sensitivity, selectivity, specificity. robustness, precision and accuracy were determined. The peak area response-concentration curve was linear, over the concentration range of 125-750 μg/mL for Metformin and 1.25-7.50 μg/mL for Empagliflozin with quantitation limits of 4.59 μg/mL for Metformin, and 0.08 μg/mL for Empagliflozin. The proposed method was successfully validated for the determination of Metformin and Empagliflozin simultaneously in combined tablet dosage form. The performance of the present method was compared with available RP-HPLC methods and found to be rapid and economical. The proposed stability-indicating RP-HPLC method can be successfully applied to quality control and drug analysis.

show abstract

QbD Stressed Development and Validation of Stability-Indicating RP- HPLC Method for the Simultaneous Estimation of Linagliptin and Metformin HCl in Pharmaceutical Dosage Form

Patel¹,

Shah²,

Joshi³

et al. 2022

RJPT

View full text Add to dashboard Cite

A new simple stability indicating reverse phase liquid chromatography method was developed by employing Quality by Design (QbD) approach for the simultaneous determination of Linagliptin and Metformin HCl. Within QbD paradigm, the present study aimed to establish the optimization of the RP-HPLC (Reverse phase high performance liquid chromatography) by means of design of experiments and response surface mythology like, Centre composite design (CCD) in order to achieve a good separation and resolution. The developed method is effective to separate Linagliptin and Metformin HCl with a good chromatographic resolution of 6.4. Chromatographic separation was acquired with column Water C18 (250mm x 4.5mm x 5μm) at flow rate 1.0 ml/min with the mobile phase consists of acetonitrile and methanol (75:25 % v/v). The detection of Linagliptin and Metformin HCl was carried out at 245nm. The proposed method was validated according to ICH guidelines. The method was linear in range of 0.5-3μg/ml and 100-600μg/ml of Linagliptin and Metformin HCl respectively and recovery were in the range of 98% to 102%. The degradation product found in stress patterns were well separated among the drug compounds. The method was validated to be specific, rapid, precise and robust for routine analysis in its pharmaceutical dosage form.

show abstract

Quantitative Estimation and Validation of Metformin Hydrochloride and Gliclazide in their Tablet Dosage Form by RP-HPLC

Cited by 6 publications

References 0 publications

Application of sulfur and nitrogen doped carbon quantum dots as sensitive fluorescent nanosensors for the determination of saxagliptin and gliclazide

Application of sulfur and nitrogen doped carbon quantum dots as sensitive fluorescent nanosensors for the determination of saxagliptin and gliclazide

Stability Indicating HPLC Method Development and Validation for Simultaneous Estimation of Metformin and Empagliflozin in Bulk and Pharmaceutical Dosage Form

QbD Stressed Development and Validation of Stability-Indicating RP- HPLC Method for the Simultaneous Estimation of Linagliptin and Metformin HCl in Pharmaceutical Dosage Form

Contact Info

Product

Resources

About