Quantitative Estimation and Validation of Rafoxanide in Presence of its Alkali-Induced Degradation Product by Different Spectrophotometric Methods

Abstract

Objective: Development and validation of four simple, accurate, selective and sensitive UV spectrophotometric methods for the determination of rafoxanide in the presence of its alkali-induced degradation product without preliminary separation. Methods: (A) Dual wavelength method, where the difference in absorbance were calculated at 242 and 281 nm. (B) Area under the curve method, where area under the peak were measured at wavelength ranges (225-235 nm) and (275-295 nm). (C) Bivariate method, where the absorbance values were measured at selected wavelengths 250 and 280 nm. (D) First derivative method, where The amplitudes were measured at 253 nm. Results: Good linearity was found in the concentration range of 2.5-25 μg/mL for all the methods. The specificity of the methods were assessed by analysing synthetic mixtures containing the drug and its degradation product. Methods are validated as per ICH guidelines and accuracy, precision, repeatability and robustness are found to be within the acceptable limit. Conclusion: The proposed methods have been found to be accurate, precise and can be used for determination of the drug in pure form and pharmaceutical formulations as well as in the presence of its degradation product without any preliminary separation steps.

Three Simple Spectrophotmetric Methods Using for Estimation of Vancomycin in Pure and Vial Injection

“…III. D1 method [15] 4-16 mg.L -1 standard solution of Vancomycin were scanned in the first derivative spectra at scan rate speed 600 nm/min, scan range "190-360 nm" and slit width 2.00 nm, using "λ=2nm" and scaling factor=20. The absorbance was measured at "200 nm valley" and was plotted against concentration to get calibration curve [16] .…”

Eco-friendly multivariate curve resolution-alternating least squares and chromatographic quantifications of some veterinary drug residues in pharmaceutical industrial wastewater

“…S1 † ) is a potent anthelmintic, chemically known as N -[3-chloro-4-(4-chloro-phenoxy)phenyl]-2-hydroxy-3,5-diiodobenzamide, widely used in veterinary medicine for the treatment of fascioliasis in sheep and cattle. 10 Different techniques were reported for RFX determination, like spectrophotometry, 11 HPLC, 12 and TLC-densitometry. 13 …”