Quantitative estimation of mimosine in anti-psoriatic ayurvedic formulation containing whole plant extract ofMimosa pudicaLinn. by validated isocratic RP-HPLC method

Abstract

In this chapter, an isocratic reverse phase HPLC determination of mimosine was developed and validated in an anti-psoriatic topically applied formulation "Lajjalu". The chromatography was performed on a C 18 column with water-orthophosphoric acid (98.8:0.2, v/v) as a mobile phase with a pH of 3.0 at a flow rate of 1.0 mL min -1 . Detection was performed at 284 nm, and a sharp peak was obtained for mimosine at a retention time of 2.62 ± 0.01 min. Linear regression analysis data for the calibration plot showed a good linear relationship between response curve and concentration in the range of 0.050-5000 ng mL −1 and the regression coefficient was 0.9998 with the linear regression equation y = 4766.8x−17726. The detection (LOD) and quantification (LOQ) limits were 10.3 and 35.6 ng mL −1 , respectively. The wide linearity range, sensitivity, accuracy, short retention time, and simple mobile phase imply the method is suitable for routine quantification of mimosine with high precision and accuracy.

Quantification of Phytochemicals and Metal Ions as well as the Determination of Volatile Compounds, Antioxidant, Antimicrobial and Antacid Activities of the Mimosa pudica L. Leaf: Exploration of Neglected and Under‐Utilized Part

“…In this study, plant identification, morphology, organoleptic evaluation, microscopic studies, and histochemical analysis, have been performed. The leaves of the Fabaceae family morphologically having compound leaves such as pinnate, bipinnate, trifoliate and rarely palmate [4,5] . The Mimosa pudica L. has bipinnate leaves which is one of the characteristics of the Fabaceae family.…”

“…So, the paracytic stomata, unicellular covering, glandular trichomes and the arrangement of palisade cells, in combination with this all, can be helpful in characterization of this particular plant. Histochemical analysis is essential to identify plant secretory structures as well as to detect metabolites of various chemical classes [4,5] . Hence, histochemical analysis imparts an important role in the characterization of the specific nature of secretory cells as well as phytoconstituents.…”

“…The leaves of the Fabaceae family morphologically having compound leaves such as pinnate, bipinnate, trifoliate and rarely palmate. [4,5] The Mimosa pudica L. has bipinnate leaves which is one of the characteristics of the Fabaceae family. The characteristic feature of this plant is nyctinastic and thigmonastic movements, which is uncommon and with this movement, a Lajjalu plant can be identified easily in the field.…”

“…There are various qualitative studies of MP leaves in different extracts, where the presences of flavonoids, alkaloids, terpenoids, carbohydrates, saponins, amino acids, phenols, tannins, proteins and steroids, while glycosides, fats, oils, resins, reducing sugars, phytosterols and phlobatannins were found to be absent [4] . Mimosine has been identified in a reverse phase high performance liquid chromatography (RP‐HPLC) analysis of the whole plant Ayurvedic Formulation [5,6] . A liquid chromatography‐mass spectrometry (LC/MS) analysis of MP leaf profiling has been reported [9]…”

“…[4] Mimosine has been identified in a reverse phase high performance liquid chromatography (RP-HPLC) analysis of the whole plant Ayurvedic Formulation. [5,6] A liquid chromatography-mass spectrometry (LC/MS) analysis of MP leaf profiling has been reported. [9] In traditional drugs, whole plant used, which is destructive technique, that may be unravel by utilizing the leaf part, which is underutilized.…”