Quantitative Estimation of Sialic Acids. III. An Anion Exchange Resin Method.

Svennerholm, Lars; Hietala, Pentti K.; Löw, H.; Siekevitz, Philip; Ernster, Lars; Diczfalusy, E.

doi:10.3891/acta.chem.scand.12-0547

Cited by 456 publications

(189 citation statements)

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“…Plates were then air-dried and gangliosides visualized with resorcinol. 46 Alternatively, the ganglioside extract was run on HPTLC aluminium-backed silica gel 60 (20 Â 20) plates (Merck). The plates were immunostained for 1 h at room temperature with GMR19 anti-GD3 MoAb (Seikagaku Corp.) and then with horseradish peroxidase (HRP)-conjugated anti-mouse IgM (Sigma Chem.…”

Section: Preparation Of Isolated Mitochondriamentioning

confidence: 99%

Lipid microdomains contribute to apoptosis-associated modifications of mitochondria in T cells

Giammarioli²,

et al. 2005

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Plasma membrane lipid microdomains have been considered as a sort of 'closed chamber', where several subcellular activities, including CD95/Fas-mediated proapoptotic signaling, take place. In this work we detected GD3 and GM3 gangliosides in isolated mitochondria from lymphoblastoid CEM cells. Moreover, we demonstrated the presence of microdomains in mitochondria by immunogold transmission electron microscopy. We also showed that GD3, the voltagedependent anion channel-1 (VDAC-1) and the fission protein hFis1 are structural components of a multimolecular signaling complex, in which Bcl-2 family proteins (t-Bid and Bax) are recruited. The disruption of lipid microdomains in isolated mitochondria by methyl-b-cyclodextrin prevented mitochondria depolarization induced by GD3 or t-Bid. Thus, mitochondrion appears as a subcompartmentalized organelle, in which microdomains may act as controllers of their apoptogenic programs, including fission-associated morphogenetic changes, megapore formation and function. These results disclose a new scenario in which mitochondria-associated lipid microdomains can act as regulators and catalysts of cell fate.

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Section: Preparation Of Isolated Mitochondriamentioning

confidence: 99%

Lipid microdomains contribute to apoptosis-associated modifications of mitochondria in T cells

Giammarioli²,

et al. 2005

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“…Phospholipids (POPC and Sm) recovery was determined by phosphorous assay using the method of Bartlett [11], GM1 ganglioside content was determined as lipid-bound sialic acid [12]. Chol content was determined as described [13].…”

Section: Liposomes Preparationmentioning

confidence: 99%

Prion protein structure is affected by pH‐dependent interaction with membranes: A study in a model system

Sesana¹,

Barbiroli²,

Bonomi³

et al. 2007

FEBS Letters

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Interaction of full length recombinant hamster prion protein with liposomes mimicking lipid rafts or non-raft membrane regions was studied by circular dichroism, chemical cross-linking and sucrose gradient ultracentrifugation. At pH 7.0, the protein bound palmitoyloleoylphosphatidylcholine/ cholesterol/sphingomyelin/monosialoganglioside GM1 (GM1) ganglioside liposomes but not palmitoyloleoylphosphatidylcholine alone (bound/free = 0.33 and 0.01, respectively), maintaining the native a-helical structure and monomeric form. At pH 5.0, though still binding to quaternary mixtures, in particular GM1, the protein bound also to palmitoyloleoylphosphatidylcholine (bound/free 0.35) becoming unfolded and oligomeric. The pH-dependent interaction with raft or non-raft membranes might have implication in vivo, by stabilizing or destabilizing the protein.

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“…The extracted gangliosides were separated by chromatography on a silicagel 60 high-performance thin-layer chromatography (HPTLC) plate (Merck, Darmstadt, Germany) using the following solvent system: chloroform/methanol/0.25% aqueous KCl (5:4:1 by volume). The gangliosides were visualized by spraying resorcinol-HCl reagent 15 and then heating at 120°C for 20 min.…”

Section: Ganglioside Analysismentioning

confidence: 99%

Ganglioside GD1a inhibits HGF-induced motility and scattering of cancer cells through suppression of tyrosine phosphorylation of c-Met

Hyuga¹,

Kawasaki²,

Hyuga³

et al. 2001

Int. J. Cancer

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We previously reported that ganglioside GD1a, which is highly expressed in poorly metastatic FBJ-S1 cells, inhibits the serum-induced motility of FBJ-LL cells and that the metastatic potential of FBJ-LL cells is completely suppressed by enforced GD1a expression (Hyuga et al., Int J Cancer 1999; 83:685-91). We recently discovered that hepatocyte growth factor (HGF) induces FBJ-LL cell motility. In the present study, the HGF-induced motility of FBJ-S1 cells was found to be one-thirtieth that of FBJ-LL cells. This motility of GD1a-expressing transfectants, which were produced by transfection of FBJ-LL cells with GM2/GD2 synthase cDNA, decreased with increases in their GD1a expression and HGF induced almost no motility in GD1a-pretreated FBJ-LL cells, indicating that GD1a inhibits the HGF-induced motility of GD1a; HGF; motility; scattering Gangliosides are sialylated glycosphingolipids found on the outer layer of the plasma membrane. Changes in ganglioside expression in cancer cells are strongly related to their metastatic potential. 1-5 Although increases in complex ganglioside expression in cancer cells have been considered to be associated with malignancy, in our previous study we found that the poorly metastatic FBJ-S1 cells highly expressed the complex ganglioside GD1a, whereas the highly metastatic FBJ-LL cells only slightly expressed this ganglioside. 6 GD1a inhibited the serum-induced motility of FBJ-LL cells and the metastatic potential of FBJ-LL cells was completely suppressed by enforced GD1a expression. 7 It is possible that GD1a suppresses metastasis of FBJ-LL cells by inhibiting cell migration. The target molecules of GD1a need to be identified to clarify the mechanism of inhibition of metastasis by GD1a. Key words:We recently found that HGF induces FBJ-LL cell motility 8 and that the serum-induced motility of FBJ-LL cells was decreased by treatment of the serum with anti-HGF antibody (unpublished data), suggesting that HGF is a migration-stimulating factor in serum. Since FBJ-LL cells metastasize to the liver and HGF is secreted by nonparenchymal liver cells, HGF may promote liver metastasis of FBJ-LL cells. We suspect that HGF may play a key role in FBJ-LL cell metastasis. HGF is a multipotential modulator of biologic activities in a variety of cell types and it influences the growth, motility, differentiation and morphogenesis of its target cells through the c-Met tyrosine kinase receptor. 9 HGF has been described as a potent chemotactic factor 10 and has been associated with the progression of carcinoma cells. 11 Overexpression of the HGF receptor, c-Met, in malignant cells has been reported in various tissues. 12 Hence HGF may play a crucial role in cancer metastasis.In the present study, we attempted to identify the target molecules of GD1a by analyzing the molecular mechanism of the inhibition of cell migration by GD1a. We found that GD1a inhibited the HGFinduced motility, morphologic change and formation of actin stress fibers in FBJ-LL cells through suppression of tyrosine phosphorylation ...

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Quantitative Estimation of Sialic Acids. III. An Anion Exchange Resin Method.

Cited by 456 publications

References 0 publications

Lipid microdomains contribute to apoptosis-associated modifications of mitochondria in T cells

Lipid microdomains contribute to apoptosis-associated modifications of mitochondria in T cells

Prion protein structure is affected by pH‐dependent interaction with membranes: A study in a model system

Ganglioside GD1a inhibits HGF-induced motility and scattering of cancer cells through suppression of tyrosine phosphorylation of c-Met

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