Quantitative evaluation of reversed-phase packing material based on calcium carbonate microspheres modified with an alternating copolymer

Kaizu, Kogi; Mochida, Mai; Imai, Hiroaki; Citterio, Daniel; Hiruta, Yuki

doi:10.1016/j.chroma.2022.463294

Cited by 4 publications

(9 citation statements)

References 40 publications

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“…Our previous work has indicated that PMAcO-modified calcium carbonate-based columns have the same level of hydrogen bonding capacity as commonly used silica gel-based columns, which is presumably due to the interaction with calcium carbonate-derived oxygen and PMAcO-derived carbonyl groups on the CaCO 3 surface. 25 Therefore, the selectivity of the Eggshell-PMAcO column for steroids might be influenced by the hydrogen bonding ability, resulting in the recognition and separation of steroids with similar log P values. Even on C18 columns where residual silanol may be present, the order of elution of steroids differs from their order of hydrophobicity.…”

Section: ■ Results and Discussionmentioning

confidence: 99%

“…20−23 We previously developed an alkali-resistant reversed-phase column packing material based on monodisperse mesoporous calcium carbonate microspheres modified with poly(maleic acid-alt-1octadecene) (CaCO 3 -PMAcO). 24,25 Since calcium carbonate is a basic oxide, the CaCO 3 -PMAcO column showed better stability than octadecyl-modified silica (ODS) columns when used with an alkaline mobile phase. In addition, the CaCO 3 -PMAcO column achieved a separation performance comparable to that of ODS columns and showed potential for the practical analysis of basic drugs under alkaline condition.…”

Section: ■ Introductionmentioning

confidence: 99%

“…In addition, the CaCO 3 -PMAcO column achieved a separation performance comparable to that of ODS columns and showed potential for the practical analysis of basic drugs under alkaline condition. 25 Therefore, by maximizing the surface properties of the biomineral through surface modification with an amphiphilic copolymer, a practical stationary phase can be expected even for calcium carbonate-based biominerals.…”

Section: ■ Introductionmentioning

confidence: 99%

“…The use of an alkaline mobile phase to prevent the ionization of basic compounds is undesirable because it reduces the durability of silica gel-based packing materials. Many studies have been performed to solve the poor separation performance of basic compounds owing to adsorption on the residual silanol groups. − We previously developed an alkali-resistant reversed-phase column packing material based on monodisperse mesoporous calcium carbonate microspheres modified with poly(maleic acid- alt -1-octadecene) (CaCO 3 -PMAcO). , Since calcium carbonate is a basic oxide, the CaCO 3 -PMAcO column showed better stability than octadecyl-modified silica (ODS) columns when used with an alkaline mobile phase. In addition, the CaCO 3 -PMAcO column achieved a separation performance comparable to that of ODS columns and showed potential for the practical analysis of basic drugs under alkaline condition .…”

Section: Introductionmentioning

confidence: 99%

“…Many studies have been performed to solve the poor separation performance of basic compounds owing to adsorption on the residual silanol groups. − We previously developed an alkali-resistant reversed-phase column packing material based on monodisperse mesoporous calcium carbonate microspheres modified with poly(maleic acid- alt -1-octadecene) (CaCO 3 -PMAcO). , Since calcium carbonate is a basic oxide, the CaCO 3 -PMAcO column showed better stability than octadecyl-modified silica (ODS) columns when used with an alkaline mobile phase. In addition, the CaCO 3 -PMAcO column achieved a separation performance comparable to that of ODS columns and showed potential for the practical analysis of basic drugs under alkaline condition . Therefore, by maximizing the surface properties of the biomineral through surface modification with an amphiphilic copolymer, a practical stationary phase can be expected even for calcium carbonate-based biominerals.…”

Section: Introductionmentioning

confidence: 99%

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Amphiphilic Copolymer-Modified Eggshell-Based Column Packing Materials for the Preparative Separation of Basic Drugs

Yoshii

Mochida

Kaizu

et al. 2022

ACS Appl. Polym. Mater.

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A way to utilize eggshells is desired because most of the eggshell waste is disposed of in landfills. Despite eggshells having attractive features such as a thermodynamically stable porous crystalline structure, their effective utilization as a functional material has yet to be demonstrated. Herein, we developed an alkali-stable column packing material for reversed-phase preparative high-performance liquid chromatography (HPLC) based on eggshells. Nanocrystals and biopolymers present on eggshell powder were removed by surface treatment with buffered ethylenediaminetetraacetic acid (EDTA) and sodium hypochlorite solutions, resulting in bare calcium carbonate with a uniform porous surface. The obtained particles were modified with poly(maleic acid-alt-1-octadecene) (PMAcO) having hydrophobic alkyl chains and anionic carboxylate groups. The prepared PMAcO-modified eggshell-based material was applied to an HPLC column, and its performance is evaluated. The column succeeded in the separation of alkylbenzenes, steroids, and diastereomers. It was also used for the separation of basic tricyclic antidepressants in an alkaline mobile phase, showing excellent retention capacity and alkali stability. This study demonstrates the potential of eggshell utilization, providing not only a practical stationary phase for HPLC but also a solution for recycling calcium carbonate-based biomineral waste.

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Section: ■ Results and Discussionmentioning

confidence: 99%

Section: ■ Introductionmentioning

confidence: 99%

Section: ■ Introductionmentioning

confidence: 99%