Quantitative event-specific multiplex PCR detection of Roundup Ready soya using LabChip technology

Abstract

EU legislation demands the use of efficient methods for the detection of genetically modified ingredients in the labelling of foodstuffs. These strict performance characteristics are most readily met with the use of "real-time" polymerase chain reaction (PCR) technologies and their associated platforms. However, the costs of the associated equipment may be beyond the scope of some molecular laboratories. This report presents a scaling technique for the determination of genetically modified content using multiplex PCR and quantitative analysis from data obtained using LabChip technology. The scaling technique is a sensitive, accurate and precise quantitative method, and is less time-consuming and uses fewer reagents than similar techniques utilising LabChip technology, as well as giving comparable results to a realtime PCR technique. This scaling technique therefore represents an alternative for genetic modification detection and quantification to that of real time PCR, with associated cost saving implications.

Recent advances in the application of capillary electromigration methods for food analysis

“…This idea can be easily understood considering the example of microchips and their recent applications in food analysis [98,[175][176][177][178][179][180][181][182][183][184][185] (see Table 4). Thus, as can be deduced from Table 4, microchips have already been applied in food analysis to differentiate species [175], to detect food spoilage bacteria [98], to detect GMOs [176,184], and to analyze small organic and inorganic compounds such as amino acids, sugars, etc [177][178][179][180][181][182][183]. Moreover, different detection schemes have been developed to be used together with microchips including LIF [98,175,176,184], conductivity [178][179][180][181], amperometric [182,183] and MS detectors [186].…”

“…Thus, as can be deduced from Table 4, microchips have already been applied in food analysis to differentiate species [175], to detect food spoilage bacteria [98], to detect GMOs [176,184], and to analyze small organic and inorganic compounds such as amino acids, sugars, etc [177][178][179][180][181][182][183]. Moreover, different detection schemes have been developed to be used together with microchips including LIF [98,175,176,184], conductivity [178][179][180][181], amperometric [182,183] and MS detectors [186]. This will make possible to address and solve an even wider number of problems in food science.…”

Sensitive and simultaneous analysis of five transgenic maizes using multiplex polymerase chain reaction, capillary gel electrophoresis, and laser‐induced fluorescence

“…However, resolution is based on melting temperatures and it has only been possible to distinguish PCR products showing T m differences higher than 1.57C [14]. Recently, commercial microfluidic electrophoresis system (LabChip  ) has been used for the analysis of triplex PCR assay to detect GM soy [15].…”

Rapid analysis of genetically modified organisms by in‐house developed capillary electrophoresis chip and laser‐induced fluorescence system

“…(v) A short PCR protocol (1 h) was performed by omitting the extension step in each amplification cycle for 35-40 cycles, contrary to previously reported protocols that are 2 h long and involve 40-50 cycles [7,8,10,11,15]. Recently, the LabChip along with the Agilent 2100 Bioanalyzer were employed with similar separation times [25]. A scaling method was described for determination of the GM content using multiplex PCR and quantitative analysis of the data obtained from the chip.…”