Quantitative Fluorescence Calibration: a Tool for Assessing the Quality of Data Obtained by Fluorescence Measurements

Vogt, Robert F.; Marti, Gerald E.; Zenger, Vincent E.

doi:10.1007/4243_2008_055

Cited by 10 publications

(2 citation statements)

References 45 publications

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“…However, fluorescence intensity commonly presented in relative units proportional to the number of emitted quanta does not have absolute meaning. Therefore, for obtaining any quantitative result, the output signal must always be calibrated as a function of analyte concentration, and if the calibration is made, it can be applied only to particular measurement in particular conditions [2]. The sources of errors and artifacts can be of different origin:…”

Section: Introduction Why We Need Wavelengthratiometric Sensing and I...mentioning

confidence: 99%

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Dual emission and its λ-ratiometric detection in analytical fluorimetry. Pt. I. Basic mechanisms of generating the reporter signal

Demchenko

2023

Methods Appl. Fluoresc.

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The wavelength-ratiometric techniques gain increasing popularity in fluorescence probing and sensing for providing inner reference to output signal and removing instrumental artefacts, in this way increasing the sensitivity and reliability of assays. Recent developments demonstrate that such approach can allow achieving much more, with the application of broad range of novel molecular and nanoscale fluorophores (luminophores), exploring the whole power of photophysical and photochemical effects and using extended range of assay formats. Simplicity of detection and potentially rich content of output data allows realizing these techniques in different simplified, miniaturized and multiplexing devices. The latter issues are discussed in Pt. II of these series.

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Section: Introduction Why We Need Wavelengthratiometric Sensing and I...mentioning

confidence: 99%

“…The results depend on the instrument, its optical configuration and performance. There can be fluctuations in the intensity of excitation light source and sensitivity of detector making unreliable the measurements of output fluorescence intensity [2].…”

Section: Introduction Why We Need Wavelengthratiometric Sensing and I...mentioning

confidence: 99%