Quantitative fluorescence lifetime spectroscopy in turbid media: comparison of theoretical, experimental and computational methods

Vishwanath, Karthik; Pogue, Brian W.; Mycek, Mary Ann

doi:10.1088/0031-9155/47/18/308

Cited by 113 publications

(86 citation statements)

References 55 publications

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“…To date, NIR techniques have been combined with several high spatial resolution, structure-specific imaging modalities including X-ray tomosynthesis 57 , ultrasound 66,74 , and MRI [75][76][77] , to study human tissues and small animals. Past experiences have contributed to a variety of imaging systems, imaging geometries, and numerical reconstruction techniques, but…”

Section: Multimodality Techniquesmentioning

confidence: 99%

Advances in Optical Spectroscopy and Imaging of Breast Lesions

Demos

Vogel

Gandjbakhche

2006

J Mammary Gland Biol Neoplasia

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Abstract:A review is presented of recent advances in optical imaging and spectroscopy and the use of light for addressing breast cancer issues. Spectroscopic techniques offer the means to characterize tissue components and obtain functional information in real time. Three-dimentional optical imaging of the breast using various illumination and signal collection schemes in combination with image reconstruction algorithms may provide a new tool for cancer detection and monitoring of treatment. 2 Chapter 1. BackgroundAccording to the National Institutes of Health, breast cancer is the most diagnosed nonskin cancer and the second leading cause of cancer death among women in the United States.Although the breast cancer diagnosis rate has increased, there has been a steady drop in the overall breast cancer death rate since the early 1990s. Depending on the size of the tumor and the involvement of the lymph nodes, survival rates can vary from 45-95%. The key problem with the breast is the variation in demographic size, texture, chemical composition, and age. X-ray mammography is the gold standard for breast cancer screening and detection. Mammography is most sensitive in women over 35-40 years of age because of their fatty breast composition 1 and less effective and sensitive in younger women because they have denser breasts 2 . For younger women, ultrasound is commonly used. Magnetic resonance imaging (MRI) can also play a significant role in the diagnosis and characterization of breast disease.According to the National Cancer Society, up to 10% of all breast cancers, roughly 20,000 cases per year in the U.S., are not discovered by X-ray mammography. Also, X-ray mammography uses ionizing radiation and requires uncomfortable breast compression. It also suffers from a significant number of false positives that often lead to unnecessary biopsy since biopsy is generally required to determine malignancy in most women with an abnormal Spatial and temporal contrasts in these properties may be uniquely useful for diagnosing disease.The objective of this work is to provide a comprehensive review of the progress of photonics methods to address breast cancer issues. This review is divided into three major focus areas: a) optical spectroscopy methods suitable for tissue evaluation in real time; b) noninvasive NIR spectroscopy for the acquisition of functional information; and c) optical imaging methods for functional tumor characterization. Chapter 2. Optical biopsy methodsThe term "optical biopsy" describes the use of optical spectroscopy to characterize tissue, and requires direct exposure of the tissue under examination to the light source. Consequently, its application in a clinical setting is best suited for intraoperative use to assist in exploring tissue in real time, or via thin fiberoptic needles that can reach the suspected location within the breast for a minimally invasive evaluation.The breast is complex; its multiple tissue components make it more difficult to classify using optical spectroscopy than other tissues (...

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Section: Multimodality Techniquesmentioning

confidence: 99%

Advances in Optical Spectroscopy and Imaging of Breast Lesions

Demos

Vogel

Gandjbakhche

2006

J Mammary Gland Biol Neoplasia

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“…However, it requires a statistically meaningful number of photons and hence can be time consuming. The Monte Carlo technique has been used by Vishwanath et al [11] to simulate time-resolved fluorescence from semi-infinite homogeneous turbid media. It was shown that the recovered lifetimes increased with increasing scattering and source-detector distance.…”

Section: Current State Of Knowledgementioning

confidence: 99%

Numerical feasibility analysis of an epidermal glucose sensor based on time-resolved fluorescence

Katika

Pilon

2006

Optical Interactions With Tissue and Cells XVII

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This paper presents numerical simulations predicting the time-resolved reflectance and autofluorescence of human skin exposed to a pulse of collimated light at 337 nm and pulse width of 1 ns. Moreover, the feasibility of using an embedded time-resolved fluorescence sensor for monitoring glucose concentration is also studied. Skin is modeled as a multilayer medium with each layer having its own optical properties and fluorophore absorption coefficients, lifetimes and quantum yields. The intensity distributions of excitation and fluorescent light in skin are then determined by solving the transient radiative transfer equation using the modified method of characteristics. In both cases, the fluorophore lifetimes are recovered from the simulated fluorescence decays and compared with the actual lifetimes used in the simulations. It was found that the fluorescence lifetime of the fluorophore contributing the least to the fluorescence signal could not be recovered while the other lifetimes could be recovered within 2.5% of input values. Such simulations could be valuable in interpreting data from time-resolved fluorescence experiments on healthy and diseased tissue as well as in designing and testing the feasibility of various optical sensors for biomedical diagnostics.

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“…The Monte Carlo code had been validated for fluorescence lifetime spectroscopy 32 . We performed calculations using a slab with a thin top layer (5 (mm)) and a much larger (semi-infinite) bottom layer.…”

Section: Nirfastmentioning

confidence: 99%

Internal refractive index changes affect light transport in tissue

et al. 2003

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This investigation explores the effect of index of refraction, as an optical property, on light transport through optically turbid media. We describe a model of light propagation that incorporates the influence of refractive index mismatch at boundaries within a domain. We measure light transmission through turbid cylindrical phantoms with different distributions of refractive index. These distributions approximate the heterogeneous, layered nature of biological tissue. Finite element method model calculations of diffuse transmittance through these phantoms show good agreement with the trends observed experimentally. We see that phase measurements of light that propagates through approximately 90 (mm) of scatter-dominated media may vary by 10 degrees depending upon the values of refractive index of the medium. Amplitude measurements are not as sensitive to this parameter as phase. Model calculations of diffuse reflectance from a semi-infinite slab geometry with different layers also shows good agreement with Monte Carlo simulations. We conclude that incorporating refractive index into light transport models may be worthwhile. Applying such a model in tomographic image reconstruction may improve the estimation of optical properties of biological tissues.

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Quantitative fluorescence lifetime spectroscopy in turbid media: comparison of theoretical, experimental and computational methods

Cited by 113 publications

References 55 publications

Advances in Optical Spectroscopy and Imaging of Breast Lesions

Advances in Optical Spectroscopy and Imaging of Breast Lesions

Numerical feasibility analysis of an epidermal glucose sensor based on time-resolved fluorescence

Internal refractive index changes affect light transport in tissue

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