Quantitative Immunoblotting Analyses Reveal that the Abundance of Actin, Tubulin, Synaptophysin and EEA1 Proteins is Altered in the Brains of Aged Mice

Ve, Hou; Cabana, Valérie C.; Gouspillou, Gilles; Lussier, Marc

doi:10.1016/j.neuroscience.2020.06.044

Cited by 13 publications

(9 citation statements)

References 88 publications

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“…49 Synaptophysin is a synaptic vesicle protein whose expression provides as a marker for synaptic density, which is also highly related to abnormal neurobehaviors. 50 According to Fig. 3D, F, and G, TMT treatment caused a significant decrease in the expression of PSD 95 and synaptophysin in the hippocampus of mice compared to that of control ones ( p < 0.01), while CGA treatment increased the protein levels of PSD 95 and synaptophysin in mouse hippocampus compared to those of TMT-treated ones ( p < 0.05).…”

Section: Resultsmentioning

confidence: 82%

Protective effects of chlorogenic acid on trimethyltin chloride-induced neurobehavioral dysfunctions in mice relying on the gut microbiota

et al. 2022

Food Funct.

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Section: Resultsmentioning

confidence: 82%

Protective effects of chlorogenic acid on trimethyltin chloride-induced neurobehavioral dysfunctions in mice relying on the gut microbiota

et al. 2022

Food Funct.

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“…Reports also demonstrated O-GlcNAc transferase (OGT) level was decreased in multiple aged tissues and suggested that dysregulation of OGT related O-GlcNAc formation might play an important role in the development of age-related diseases ( Fulop et al, 2008 ). Researchers also reported the abundance of EEA1 proteins was altered in the brains of aged mice ( Ve et al, 2020 ). Moreover, SRP68 has been reported for its association with cellular senescence, while the ubiquitination-related genes RNF25 is not clear in immune aging.…”

Section: Resultsmentioning

confidence: 99%

“…Researchers also reported the abundance of EEA1 proteins was altered in the brains of aged mice (Ve et al, 2020). Moreover, SRP68 has been reported for its association with cellular senescence, while the ubiquitination-related genes RNF25 is not clear in immune aging.…”

Section: Aging and Race Cause Transcriptomic Variations Over Human Ad...mentioning

confidence: 99%

Gene Expression Analysis Reveals Age and Ethnicity Signatures Between Young and Old Adults in Human PBMC

Yang

Mao

et al. 2022

Front. Aging

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Human immune system functions over an entire lifetime, yet how and why the immune system becomes less effective with age are not well understood. Here, we characterize peripheral blood mononuclear cell transcriptome from 132 healthy adults with 21–90 years of age using the weighted gene correlation network analyses. In our study, 113 Caucasian from the 10KIP database and RNA-seq data of 19 Asian (Chinese) are used to explore the differential co-expression genes in PBMC aging. These two dataset reveal a set of insightful gene expression modules and representative gene biomarkers for human immune system aging from Asian and Caucasian ancestry, respectively. Among them, the aging-specific modules may show an age-related gene expression variation spike around early-seventies. In addition, we find the top hub genes including NUDT7, CLPB, OXNAD1, and MLLT3 are shared between Asian and Caucasian aging related modules and further validated in human PBMCs from different age groups. Overall, the impact of age and race on transcriptional variation elucidated from this study may provide insights into the transcriptional driver of immune aging.

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“…Reports also demonstrated O-GlcNAc transferase (OGT) level was decreased in multiple aged tissues and suggested that dysregulation of OGT related O-GlcNAc formation might play an important role in the development of age-related diseases [9]. Researchers also reported the abundance of EEA1 proteins was altered in the brains of aged mice [10]. Moreover, SRP68 has been reported for its association with cellular senescence, while the ubiquitination-related genes RNF25…”

Section: Novel and Known Age-associated Genes And Pathways Associated With Pbmc Aging In Asian (Chinese)mentioning

confidence: 99%

Identification of age and ethnicity specific gene expression biomarkers for immune aging

Yang¹,

Xu²,

Mao³

et al. 2021

Preprint

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Human immune system functions over an entire lifetime, yet how and why the immune system becomes less effective with age are not well understood. Here, we characterize peripheral blood mononuclear cells from 172 healthy adults 21~90 years of age using RNA-seq and the weighted gene correlation network analysis (WGCNA). These data have revealed a meaningful of gene expression modules or representative biomarkers for human immune system aging in Aisan and White ancestry. Among them, several gene modules demonstrated a remarkably correlation with human immune aging progress. Besides, further analysis on these ageing related modules show age-related gene expression changes spike is around early-seventies. More importantly, we also focus on how race and ethnicity affect immune aging as race-specific effects on these gene expression changes have clinical applications for diagnosis and interpretation of immunosenescence. Thus, the top hub genes including NUDT7, CLPB, OXNAD1 and MLLT3 are identified from Asian and white aging related modules and further validated in humans PBMC at different ages. Finally, the impact of age and race on immune phenotypes we discuss may provide insights into future studies.

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Quantitative Immunoblotting Analyses Reveal that the Abundance of Actin, Tubulin, Synaptophysin and EEA1 Proteins is Altered in the Brains of Aged Mice

Cited by 13 publications

References 88 publications

Protective effects of chlorogenic acid on trimethyltin chloride-induced neurobehavioral dysfunctions in mice relying on the gut microbiota

Protective effects of chlorogenic acid on trimethyltin chloride-induced neurobehavioral dysfunctions in mice relying on the gut microbiota

Gene Expression Analysis Reveals Age and Ethnicity Signatures Between Young and Old Adults in Human PBMC

Identification of age and ethnicity specific gene expression biomarkers for immune aging

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