Quantitative inhibitory influence of porcine cumulus cells upon the maturation of pig and cattle oocytes in vitro

Petr, Jaroslav; Zetová, L; Fulka, J.; Jílek, F.

doi:10.1051/rnd:19890503

Cited by 20 publications

(10 citation statements)

References 21 publications

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“…As described in our previous study (Petr et al, 1989), porcine cumulus cells produce at least one factor quantitatively inhibiting germinal vesicle breakdown (GVBD) in pig oocytes. This inhibitory factor acts indirectly on oocytes through the mediation of cumulus cells.…”

mentioning

confidence: 77%

“…It was suggested by Dekel and Sherizly (1983) (Dekel et al, 1988) and rabbits (Hosoi et al, 1989) (Petr et al, 1989) and LH is known to induce oocyte maturation through its effect on the somatic follicular periovarian cells (Dekel and …”

Section: Methodsmentioning

confidence: 99%

“…

Summary &horbar; The factor (s) produced by porcine cumulus cells (cumulus cell factor (s): CCF) was described as quantitatively inhibiting the maturation of oocytes in vitro (Petr at al, 1989

…”

mentioning

confidence: 99%

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Influence of dbcAMP on the inhibitory effect of cumulus cell factor(s)

Petr¹,

Zetová²,

Fulka³

1991

Reprod. Nutr. Dévelop.

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mentioning

confidence: 77%

Section: Methodsmentioning

confidence: 99%

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Influence of dbcAMP on the inhibitory effect of cumulus cell factor(s)

Petr¹,

Zetová²,

Fulka³

1991

Reprod. Nutr. Dévelop.

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“…As we described previously (Petr ef al, 1989;Petr et al, 1991) (Petr et al, 1989;Petr et al, 1991 ).…”

Section: Discussionmentioning

confidence: 99%

“…In our previous studies, we have demonstrated the presence of a factor produced by cumulus cells from fully grown porcine oocytes, which inhibits GVBD in fully grown pig and cattle oocytes (Petr et al, 1989). This factor is released into the culture medium and its inhibitory effect can be overcome by the addition of LH, heparin or by the transient exposure of oocytes to dbcAMP (Petr et al, 1991).…”

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confidence: 96%

Inhibition of meiotic maturation in growing pig oocytes by factor(s) from cumulus cells

Petr¹,

Teplá²,

Grocholová³

et al. 1994

Reprod. Nutr. Dev.

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Summary ― Total inhibition of germinal vesicle breakdown (GVBD) was observed in growing pig oocytes (internal diameter 80, 90 and 100 11m) when they were cultured in a medium conditioned by cumulus oocyte complexes (COCs) of fully grown oocytes. In denuded growing oocytes, only partial inhibition was observed. The inhibitory effect was fully reversible. The addition of heparin (300 IU/ml) could overcome the effect of the conditioned medium. Transient exposure (6 h) of oocytes to dibutyryl cyclic adenosine monophosphate (dbcAMP) (1 mg/mi) could also partly reverse the effect of factor (s) produced by cumulus cells of fully grown oocytes. Follicle-stimulating hormone (5 I1g/ml) was able to increase the percentage of maturing oocytes. The addition of luteinizing hormone (5 I1g/ml) had no effect on GVBD inhibition by cumulus-conditioned medium.growing oocyte / maturation / cumulus cells / pig Résumé ― Inhibitition de la maturation méiotique dans les ovocytes en croissance par des facteurs des cellules du cumulus. La rupture de la vésicule germinative est inhibée dans des ovocytes en croissance de porc (diamètre interne: 80, 90 et 100 ¡lM) cultivés dans un milieu conditionné avec des complexes cumuluslovocytes après croissance. Dans les ovocytes en croissance dénudés, c'est seulement une inhibition partielle qui se produit. L'inhibition est complètement réversible : l'addition d'héparine (300 UIlml) peut renverser l'effet du milieu conditionné. Une exposition transitoire (6 h) des ovocytes à 1 mglml de dibutyryl AMPc (dibutyryl cyclic adénosine monophosphate) est aussi capable de renverser partiellement l'effet des facteurs produits par les cellules du cumulus d'ovocytes après croissance. L'hormone folliculo-stimulante (5 wglml) augmente le taux de maturation des ovocytes. L'addition d'hormone lutéinisante (5¡ l g/ml) n'a pas d'effet sur l'inhibition de la rupture de la vésicule germinative par le milieu conditionné.

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Inhibitory action of hypoxanthine on meiotic resumption of denuded pig follicular oocytes in vitro

Miyano

Ebihara²,

Goto

et al. 1995

J. Exp. Zool.

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The present study was conducted to investigate the direct action of hypoxanthine, which has been reported to be present in pig follicular fluid, on spontaneous meiotic resumption of pig denuded oocytes in vitro. No oocytes, which were surrounded by a cumulus and some membrana granulosa cells directly adjacent to it from healthy antral follicles, underwent germinal vesicle breakdown (GVBD) when they were cultured in serum-supplemented 199 with or without 4 mM hypoxanthine. On the other hand, 81% of denuded oocytes prepared from such cumulus-oocyte-granulosa cell complexes showed GVBD after 24 h of culture. Hypoxanthine inhibited significantly this spontaneous resumption of meiosis in denuded oocytes. The inhibitory action of hypoxanthine was dose dependent at concentrations of 1-6 mM and was reversible following subsequent culture of the oocytes in hypoxanthine-free medium. However, premature chromosome condensation was observed in oocytes cultured in hypoxanthine-supplemented medium, and the oocytes released from the influence of hypoxanthine completed GVBD and reached the second metaphase slightly faster than those freshly isolated from the follicle. These results suggest that hypoxanthine inhibits spontaneous meiotic resumption of pig oocytes in vitro. However, the inhibitory action of hypoxanthine does not prevent all events involved in GVBD from occurring.

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Quantitative inhibitory influence of porcine cumulus cells upon the maturation of pig and cattle oocytes in vitro

Abstract: Summary ― Porcine cumulus oocyte complexes (COCs) were cultured together in 10-)ii droplets of culture medium. When 10 COCs were cultured for 24 h, germinal vesicle breakdown (GVBD)

Cited by 20 publications

References 21 publications

Influence of dbcAMP on the inhibitory effect of cumulus cell factor(s)

Influence of dbcAMP on the inhibitory effect of cumulus cell factor(s)

Inhibition of meiotic maturation in growing pig oocytes by factor(s) from cumulus cells

Inhibitory action of hypoxanthine on meiotic resumption of denuded pig follicular oocytes in vitro

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