Quantitative mass spectrometry imaging (qMSI): A tutorial

Kibbe, Russell R.; Muddiman, David C.

doi:10.1002/jms.5009

Cited by 7 publications

(2 citation statements)

References 64 publications

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“…This method utilized an untreated kidney tissue sample which was sectioned and mounted on a clean glass slide. An on-tissue calibration curve method for qMSI was followed ( Kibbe and Muddiman, 2024 ). To investigate the linear range of the mass spectrometer, a calibration curve was generated by preparing a blank sample and nine calibrants at 5, 10, 20, 30, 40, 50, 70, 90, and 100 ng/μL via serial dilution of the rotenone standard solution in MeOH.…”

Section: Methodsmentioning

confidence: 99%

Visualization of renal rotenone accumulation after oral administration and in situ detection of kidney injury biomarkers via MALDI mass spectrometry imaging

Tenebro,

Marcial,

Salcepuedes

et al. 2024

Front. Mol. Biosci.

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The examination of drug accumulation within complex biological systems offers valuable insights into the molecular aspects of drug metabolism and toxicity. Matrix-assisted laser desorption/ionization mass spectrometry imaging (MALDI MSI) is an innovative methodology that enables the spatial visualization and quantification of biomolecules as well as drug and its metabolites in complex biological system. Hence, this method provides valuable insights into the metabolic profile and any molecular changes that may occur as a result of drug treatment. The renal system is particularly vulnerable to adverse effects of drug-induced harm and toxicity. In this study, MALDI MSI was utilized to examine the spatial distribution of drug and renal metabolites within kidney tissues subsequent to a single oral dosage of the anticancer compound rotenone. The integration of ion mobility spectrometry with MALDI MSI enhanced the data acquisition and analysis, resulting to improved mass resolution. Subsequently, the MS/MS fragment ions of rotenone reference drug were detected and characterized using MALDI HDMS/MS imaging. Notably, drug accumulation was observed in the cortical region of the representative kidney tissue sections treated with rotenone. The histological examination of treated kidney tissues did not reveal any observable changes. Differential ion intensity of renal endogenous metabolites was observed between untreated and rotenone-treated tissues. In the context of treated kidney tissues, the ion intensity level of sphingomyelin (D18:1/16:0), a sphingolipid indicator of glomerular cell injury and renal damage, was found to be elevated significantly compared to untreated kidney tissues. Conversely, the ion intensities of choline, glycero-3-phosphocholine (GPC), inosine, and a lysophosphatidylcholine LysoPC(18:0) exhibited a significant decrease. The results of this study demonstrate the potential of MALDI MSI as a novel technique for investigating the in situ spatial distribution of drugs and renal endogenous molecules while preserving the anatomical integrity of the kidney tissue. This technique can be used to study drug-induced metabolism and toxicity in a dynamic manner.

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Section: Methodsmentioning

confidence: 99%

Visualization of renal rotenone accumulation after oral administration and in situ detection of kidney injury biomarkers via MALDI mass spectrometry imaging

Tenebro,

Marcial,

Salcepuedes

et al. 2024

Front. Mol. Biosci.

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show abstract

“…This method utilized an untreated kidney tissue sample which was sectioned and mounted on a clean glass slide. An on-tissue calibration curve method for qMSI was followed (Kibbe and Muddiman, 2024). To investigate the linear range of the mass spectrometer, a calibration curve was generated by preparing a blank sample and nine calibrants at 5,10,20,30,40,50,70,90, and 100 ng/μL via serial dilution of the rotenone standard solution in MeOH.…”

Section: Quantitative Msi (Qmsi) Analysismentioning

confidence: 99%

DataSheet1.pdf

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Absolute Quantification of Glutathione Using Top‐Hat Optics for IR‐MALDESI Mass Spectrometry Imaging

Bruce,

Kibbe,

Hector

et al. 2024

J Mass Spectrom

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Infrared matrix‐assisted laser desorption electrospray ionization (IR‐MALDESI) uses an infrared laser to desorb neutral biomolecules with postionization via ESI at atmospheric pressure. The Gaussian profile of the laser with conventional optics results in the heating of adjacent nonablated tissue due to the energy profile being circular. A diffractive optical element (DOE) was incorporated into the optical train to correct for this disadvantage. The DOE produces a top‐hat beam profile and square ablation spots, which have uniform energy distributions. Although beneficial to mass spectrometry imaging (MSI), it is unknown how the DOE affects the ability to perform quantitative MSI (qMSI). In this work, we evaluate the performance of the DOE optical train against our conventional optics to define the potential advantages of the top‐hat beam profile. Absolute quantification of glutathione (GSH) was achieved by normalizing the analyte of interest to homoglutathione (hGSH), spotting a dilution series of stable isotope labeled glutathione (SIL‐GSH), and analyzing by IR‐MALDESI MSI with either the conventional optical train or with the DOE incorporated. Statistical comparison indicates that there was no significant difference between the quantification of GSH by the two optical trains as evidenced by similar calibration curves. Results support that both optical trains can be used for qMSI without a change in the ability to carry out absolute quantification but providing the benefits of the top‐hat optical train (i.e., flat energy profile and square ablation spots)—for future qMSI studies.

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Quantitative mass spectrometry imaging (qMSI): A tutorial

Cited by 7 publications

References 64 publications

Visualization of renal rotenone accumulation after oral administration and in situ detection of kidney injury biomarkers via MALDI mass spectrometry imaging

Visualization of renal rotenone accumulation after oral administration and in situ detection of kidney injury biomarkers via MALDI mass spectrometry imaging

DataSheet1.pdf

Absolute Quantification of Glutathione Using Top‐Hat Optics for IR‐MALDESI Mass Spectrometry Imaging

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