Quantitative microemulsion electrokinetic capillary chromatography analysis of formulated drug products

Mahuzie, Pierre-Etienne; Clark, Brian J.; Crumpton, Andy J.; Altria, Kevin D.

doi:10.1002/1615-9314(20010901)24:9<784::aid-jssc784>3.0.co;2-2

Cited by 28 publications

(6 citation statements)

References 20 publications

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“…The study described in this paper was performed with five acidic drugs (NSAIDs): ibuprofen, naproxen, fenoprofen, diclofenac sodium, and ketoprofen. Some of these compounds have been individually analyzed using a micro-emulsion BGE solution [1,2,22]. Since at low pH these drugs are water-insoluble compounds, they are more likely a part of the oil droplet than the aqueous buffer phase.…”

Section: Introductionmentioning

confidence: 99%

Separation and on‐column preconcentration of some nonsteroidal anti‐inflammatory drugs by microemulsion electrokinetic capillary chromatography using high‐speed separations

et al. 2005

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Various strategies have been investigated for separating a group of nonsteroidal anti-inflammatory drugs (NSAIDs) by microemulsion electrokinetic capillary chromatography (MEEKC) using high-speed separations. The parameters that of affect the separation, such as the nature of the oil droplet and the buffer, and the surfactant concentration have been studied. In addition, several organic solvents were used to decrease the retention of the analytes in the oil droplet phase and to improve the resolution of the NSAIDs. The optimum microemulsion background electrolyte (BGE) solution made of 0.8% w/w ethyl acetate, 6.6% w/w butan-1-ol, 6.0% w/w acetonitrile, 1.0% w/w sodium dodecyl sulfate (SDS), and 85.6% w/w of 10 mM sodium tetraborate at pH 9.2 resolved the drugs within 8 min. The short-end injection procedure is an alternative for reducing the analysis time. When this procedure was used, the microemulsion BGE solution consisted of 0.8% w/w ethyl acetate, 6.6% w/w butan-1-ol, 17.0% w/w methanol, 1.0% w/w SDS, and 74.6% w/w of 10 mM sodium tetraborate, pH 9.2, and the NSAIDs were separated within 3 min. The reversed electrode polarity stacking mode (REPSM) technique was applied to the on-line concentration of the NSAIDs. In this technique, the sample matrix was pumped out of the capillary using a polarity-switching step. When this technique was applied, the sensitivity was enhanced up to 40-fold and the limits of detection (LODs) were in the low microg.L(-1) levels.

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Section: Introductionmentioning

confidence: 99%

Separation and on‐column preconcentration of some nonsteroidal anti‐inflammatory drugs by microemulsion electrokinetic capillary chromatography using high‐speed separations

et al. 2005

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“…bubble or Z-cells) were used. Mahuzier et al (2001) demonstrated the poor sensitivity of CE based methods, in comparison to other separation methods. The problem of limited sensitivity of CE methods can be solved either by the use of detection methods with sensitivity higher than UV absorption or by pre-concentration of the analytes (Jouyban and Kenndler, 2008).…”

Section: Tlc/hptlc Methodsmentioning

confidence: 99%

Genotoxic Impurities in Pharmaceuticals

Jouyban¹,

Parsa²

2012

Toxicity and Drug Testing

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“…13–15 Stability indicating method for the determination of mixture of rizatriptan and naproxen was established using microemulsion electrokinetic chromatography (MEEC). 16…”

Section: Introductionmentioning

confidence: 99%

Two chromatographic Methods for the Determination of some Antimigraine Drugs

2012

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Two stability indicating chromatographic methods were proposed for the determination of almotriptan, eletriptan, and rizatriptan, in presence of their acid degradation products. The first method is a quantitative densitometric thin layer chromatography. The developing systems were; acetonitrile: methanol: dichloromethane: ammonia (10:6:3:1 v/v), ethyl acetate: methanol: ammonia (15:4:1 v/v), and methanol: acetonitrile: ammonia (9:4:1 v/v) for almotriptan, eletriptan and rizatriptan respectively. The TLC plates were scanned at 235 nm. Linear relationships were obtained over concentration ranges (5–50 μg/spot) for almotriptan and rizatriptan, and (5–60 μg/spot) for eletriptan. The second method based on the separation and determination of the studied drugs, using RP-HPLC technique. The separation was achieved on C18 Hypersil column, elution was carried out using phosphate buffer pH 3: methanol: acetonitrile (2: 1:1 v/v) at flow rate 2 mL/min and UV detection at 235 nm. Linear relationships were obtained over concentration ranges (10–200 μg/mL) for almotriptan and eletriptan, and (10–180 μg/mL) for rizatriptan. The chromatographic methods were successfully applied for the determination of each of the studied drugs in pure form, tablet form, and in laboratory prepared mixtures with their acid degradation products.

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Quantitative microemulsion electrokinetic capillary chromatography analysis of formulated drug products

Cited by 28 publications

References 20 publications

Separation and on‐column preconcentration of some nonsteroidal anti‐inflammatory drugs by microemulsion electrokinetic capillary chromatography using high‐speed separations

Separation and on‐column preconcentration of some nonsteroidal anti‐inflammatory drugs by microemulsion electrokinetic capillary chromatography using high‐speed separations

Genotoxic Impurities in Pharmaceuticals

Two chromatographic Methods for the Determination of some Antimigraine Drugs

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