Quantitative mRNA expression of sox3 and DMRT1 during sex reversal, and expression profiles after GnRHa administration in black porgy, Acanthopagrus schlegeli

Shin, Hyun Suk; An, Kwang Wook; Park, Mi Seon; Jeong, Min Hwan; Choi, Cheol Young

doi:10.1016/j.cbpb.2009.05.013

Cited by 32 publications

(19 citation statements)

References 35 publications

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“…In addition, sox3 expression was higher at the spawning phase where spermatogenesis and spermiation processes occur. Similar to the present study, sox3 expression was higher in mature testis than in the ovary of Acanthopagrus schlegeli [Shin et al, 2009]. The role of Sox3 in neural development and function has been well established [Wood and Episkopou, 1999;Dee et al, 2008] and has been shown to be a permissive factor for sensory placode formation, which plays an important role in sensory organ development in O. latipes [Koster et al, 2000].…”

Section: Discussionsupporting

confidence: 77%

“…Expression of sox3 increased after hCG administration both in vivo and in vitro to maximum levels at 24 and 6 h, respectively. The hCG-induced sox3 expression in catfish was more similar to the gonadotropin-releasing hormone (GnRH) analogue-induced expression in A. schlegeli [Shin et al, 2009], indicating a GnRH dependency. Sox3 protein was expressed in germ cells [Raverot et al, 2005;Yao et al, 2007;Laronda and Jameson, 2011] and Sertoli cells of testes in Mus musculus and A. schlegeli [Weiss et al, 2003;Yao et al, 2007].…”

Section: Discussionmentioning

confidence: 80%

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Expression Analysis of sox3 during Testicular Development, Recrudescence, and after hCG Induction in Catfish, Clarias batrachus

Anbazhagan¹,

Senthilkumaran²

2014

Sex Dev

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In teleosts, the expression of steroidogenic enzymes and related transcription factor genes occurs in a stage- and tissue-specific manner causing sexual development. The role of sox3, an evolutionary ancestor of SRY, has not been studied in detail. Therefore, the full-length cDNA of sox3 (1,197 kb) was cloned from catfish testis, and mRNA expression was analyzed during gonadal development, during the seasonal reproductive cycle, and after human chorionic gonadotropin (hCG) induction. Tissue distribution analysis showed that sox3 expression was higher in testis, ovary, and brain compared to other tissues analyzed. Developing and mature testis showed higher sox3 expression than ovary of corresponding stages, and more sox3 transcripts were found during the spawning phase of the seasonal reproductive cycle. Expression of sox3 was upregulated by hCG after in vivo and in vitro induction, suggesting that gonadotropins might stimulate it. In situ hybridization and immunohistochemistry showed the presence of sox3 mRNA and protein in somatic and interstitial cell layers of the testis. Sox3 could also be found in the zona radiata of developing and mature oocytes. Exposure of methyltestosterone (1 µg/l) and ethinylestradiol (1 µg/l) for 21 days during testicular development showed lower sox3 expression levels in the testis and brain, indicating a certain feedback intervention. These results suggest a possible role for Sox3 in the regulation of testicular development and function.

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Section: Discussionsupporting

confidence: 77%

Section: Discussionmentioning

confidence: 80%

Expression Analysis of sox3 during Testicular Development, Recrudescence, and after hCG Induction in Catfish, Clarias batrachus

Anbazhagan¹,

Senthilkumaran²

2014

Sex Dev

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“…In contrast, during the ovarian cycle, expression of sox9b transcript was abundant in the preparatory phase when compared with the prespawning phase, spawning, and post-spawning phases. Recent report showed that Gnrh analog treatment (in vitro and in vivo) up-regulates sox3 and dmrt1 mRNA expression in immature black porgy (Shin et al 2009). In another study, it was observed that FSH could regulate Dmrt1 expression in postnatal rat testis (Chen & Heckert 2001).…”

Section: Discussionmentioning

confidence: 99%

Isolation of sox9 duplicates in catfish: localization, differential expression pattern during gonadal development and recrudescence, and hCG-induced up-regulation of sox9 in testicular slices

Kavarthapu¹,

Senthilkumaran²

2010

Reproduction

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In vertebrates, sox9 is a transcription factor that plays a crucial role in testicular development and chondrogenesis. Here, we report cloning of isoforms of sox9 (sox9a and sox9b) from air-breathing catfish Clarias gariepinus, which undergoes an annual reproductive cycle. Tissue distribution pattern showed differential expression of sox9 duplicates, wherein both forms were highly expressed in brain and gonads. Furthermore, we observed a dimorphic expression pattern of sox9a and sox9b in both adult and developing gonads using RT-PCR, indicating that sox9a retained its function in testis while sox9b might have a new role to play in ovary. Changes in sox9 mRNA levels using real-time quantitative PCR (qRT-PCR) during the seasonal reproductive cycle revealed that sox9a transcript in testis was abundant during testicular recrudescence (during spermatogenesis), and its expression significantly decreased during spawning and post-spawning phases. Furthermore, treatments of human chorionic gonadotropin and 11-ketotestosterone in vitro up-regulated sox9a mRNA levels in the testicular slices at 12 and 24 h time points, suggesting that gonadotropins might stimulate sox9 expression. These results suggest that sox9 might have a plausible role in the entrainment of the testicular cycle. In contrast, during the ovarian cycle, sox9b mRNA levels gradually declined from preparatory to post-spawning phases. Immunohistochemical (IHC) data showed that, in testis, sox9 is detectable in Sertoli and spermatogonial cell types except spermatid/spermatozoa. In the ovary, it is localized in the ooplasm of primary and pre-vitellogenic oocytes. These results were further confirmed by whole-mount IHC and qRT-PCR.

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“…Gen pengkode untuk analisis ekspresi gen reproduksi meliputi DMRT1 yang merupakan gen pengkode yang berperan dalam proses pembentukan sperma dan SOX3 yang merupakan gen yang terkait dalam proses pembentukan sel telur. Tahapan analisis ekspresi gen terkait gen reproduksi sesuai Haryanti et al (2014), target gen spesifik mengikuti Shin et al (2009) seperti tertera pada Tabel 1.…”

Section: Ekspresi Gen Dmrt1 Dan Sox3unclassified

“…Nampaknya, induksi hormon pada ikan kerapu sunu dapat mengekspresikan peran gen DMRT1 dan SOX3 sebagai pengkode dalam perkembangan dan pematangan gonad jantan. Hal ini sesuai dengan peranan DMRT1 dan SOX3 pada black porgy Acanthopagrus schlegeli yang menunjukkan ekspresi maksimal pada gonad jantan yang mulai matang (Shin et al, 2009).…”

Section: Ekspresi Gen Yang Terkait Dengan Reproduksiunclassified

PENGARUH RANGSANGAN HORMON AROMATASE INHIBITOR DAN OODEV TERHADAP PERUBAHAN KELAMIN DAN PERKEMBANGAN GONAD IKAN KERAPU SUNU, Plectropomus leopardus

Yudha

Sudrajat

Haryanti

2018

Jurnal Riset Akuakultur

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Permasalahan utama yang dihadapi dalam penyediaan calon induk ikan kerapu sunu Plectropomus leopardus hasil budidaya yang bersifat hermaprodit sekuensial adalah keterlambatan dalam perkembangan gonad dan perubahan gonad dari betina menjadi jantan. Manipulasi hormonal merupakan cara yang paling efektif dan efisien dalam memacu perkembangan reproduksi dan pematangan gonad. Tujuan dari penelitian ini adalah untuk mendapatkan dosis hormon aromatase inhibitor dan oodev yang tepat untuk memacu perubahan kelamin dan perkembangan gonad ikan kerapu sunu. Ikan uji yang digunakan sebanyak 35 ekor F-1 dengan bobot rerata 2,3 ± 0,28 kg. Penelitian dilakukan selama dua bulan. Induksi hormon dilakukan melalui penyuntikan setiap dua minggu sekali dengan empat dosis aromatase inhibitor dan oodev yang berbeda; A (aromatase inhibitor 1 mg kg-1 ikan), O (oodev 1 mL kg-1 ikan), AO1 (aromatase inhibitor 0,1 mg kg-1 ikan + oodev 1 mL kg-1 ikan), AO2 (aromatase inhibitor 1 mg kg-1 ikan + oodev 1 mL kg-1 ikan), dan K (plasebo). Hasil penelitian menunjukkan bahwa kombinasi aromatase inhibitor 1 mg kg-1 ikan dan oodev 1 mL kg-1 ikan efektif untuk merangsang perubahan kelamin. Perlakuan tersebut dapat meningkatkan konsentrasi testosteron dalam darah (2,819 ng/mL) setelah delapan minggu pemeliharaan. Berdasarkan hasil histologi gonad dan observasi terhadap ekspresi gen terkait reproduksi menggunakan gen target DMRT1 dan SOX3 menunjukkan bahwa perlakuan hormon AO2 (Aromatase inhibitor 1 mg kg-1 ikan + oodev 1 mL kg-1 ikan) terbukti dapat memacu perubahan kelamin dari betina menjadi jantan dan kematangan gonad pada ikan kerapu sunu Plectropomus leopardus.The main problems faced in providing prospective broodstock of protogynous hermaphrodite coral trout grouper Plectropomus leopardus are lateness of gonadal development and gonadal sex reversal from female to male. Hormonal manipulation is the most effective way to induce reproductive development and gonadal maturation. The present study aimed to determine an effective dose of aromatase inhibitor and oodev on sex reversal and gonadal development of coral trout grouper. There were 35 F-1 fish with an average weight of 2.3 ± 0.28 kg. This research was conducted for two months. The fish were injected with four different dosages of aromatase inhibitor and oodev every two weeks: A (aromatase inhibitor 1 mg kg-1 fish), O (1 mL oodev), AO1 (aromatase inhibitor 0.1 mg kg-1 fish + oodev 1 mL kg-1 fish), AO2 (aromatase inhibitor 1 mg kg-1 fish + oodev 1 mL kg-1 fish), and K (placebo). The results showed that the combination of aromatase inhibitor 1 mg kg-1 fish and oodev 1 mL kg-1 fish was effective to induce the sex change. It could increase the concentration of testosterone in the blood (2.819 ng/mL) after eight weeks of culture. Based on the results of gonadal histology and reproductive-related genes expression observations using DMRT1 and SOX3 target genes, the hormonal treatment of AO2 (aromatase inhibitor 1 mg kg-1 fish + oodev 1 mL kg-1 fish) was able to accelerate sex reversal from female to male and gonadal maturation in coral trout grouper Plectropomus leopardus.

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Quantitative mRNA expression of sox3 and DMRT1 during sex reversal, and expression profiles after GnRHa administration in black porgy, Acanthopagrus schlegeli

Cited by 32 publications

References 35 publications

Expression Analysis of <b><i>sox3</i></b> during Testicular Development, Recrudescence, and after hCG Induction in Catfish, <b><i>Clarias batrachus</i></b>

Expression Analysis of <b><i>sox3</i></b> during Testicular Development, Recrudescence, and after hCG Induction in Catfish, <b><i>Clarias batrachus</i></b>

Isolation of sox9 duplicates in catfish: localization, differential expression pattern during gonadal development and recrudescence, and hCG-induced up-regulation of sox9 in testicular slices

PENGARUH RANGSANGAN HORMON AROMATASE INHIBITOR DAN OODEV TERHADAP PERUBAHAN KELAMIN DAN PERKEMBANGAN GONAD IKAN KERAPU SUNU, Plectropomus leopardus

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