Quantitative Parameters and Morphological Characteristics of the Uterine Tubes Ampullae in the First Period of Adulthood

Abstract

The study of the age-related anatomical variability, its exact morphological characteristics in adulthood, established using modern research methods, are needed, which can serve as a criterion for a conditional norm. The aim of the study is to determine the macro- and micro-morphometric parameters of the ampulla of the fallopian tubes of women in the first period of adulthood using prosection material and their morphological characteristics. The ampulla of both fallopian tubes of 44 nulliparous and 58 giving birth women of the first period of adulthood (from 21 to 35 years) were examined. Organometric, histological, immunohistochemical and macro- and micro-morphometric research methods were used. It determined the length of the ampoules, their outer diameter at the junction of the isthmus in the ampoule, in the central part of the ampoule and at the junction of the ampoule in the funnel. Histological sections were stained with hematoxylin and eosin, picro fuchsin according to van Gieson. In the immunohistochemical study of the samples, antibodies against vimentin and the CD68 marker were used. The area of ​​the lumen of the ampulla of the fallopian tube and the area of ​​its wall was calculated, and the circumference of the epithelial lining was measured. As a result, it was found that the outer diameter of the fallopian tubes at the junction of the isthmus into the ampulla and the ampoule into the funnel, as well as in the central part of the ampoule, is characterized by the absence of a statistically significant difference in parameters in nulliparous and giving birth women. The histological picture of the ampulla of the fallopian tubes both in nulliparous and in women giving birth in the first period of adulthood is of the same type and is represented by a mucous membrane that forms many thin papillary folds, internal circular and external longitudinal layers of smooth muscle tissue of the muscular membrane and the serous membrane. The detection in the tissues of the ampulla of the fallopian tubes in nulliparous and giving birth women in a moderate amount of CD68-immunopositive macrophages, the concentration of which increases in the epithelium and near it, as well as the expression of vimentin in the vessel walls and individual fibroblasts, indicates the presence of physiological tissue regeneration processes. Analysis of the indicators of the areas of the lumen of the ampoule and its wall at the median section, as well as the circumference of the epithelial lining, allows us to conclude that these parameters predominate in the right fallopian tube.