The chemical and biological profiling of the methanolic extract of the floral part of Costus lucanisianus have been carried out. The floral part was collected from Ogbia Local Government Area of Bayelsa State. The floral part was air dried, grinded and extracted at room temperature in methanol. The methanol extract mixture was then concentrated under pressure. The concentrated extract, MCL was subjected to quantitative phytochemical screening, antioxidant and antimicrobial profiling. The result of the quantitative analysis showed the presence of phenols (14.853±0.350), tannins (6.638±0.051), alkaloids (3.72±0.02), flavonoids (59.444±0.000) and saponins (2.93±0.06). The antioxidant activity increases as the concentration of the methanolic extract increases. Biologically, the floral part has shown activities against the following strains of microbes: Staphylococcus aureus, Bacillus subtillis, Escherichia coli, Pseudomonas aeruginosa, Salmonella typhi, Candida albican, Klebsiela pneumonia, and Fusarium spp. with the highest activity observed against Staphylococcus aureus. The least antimicrobial activity was observed for Escherichia coli. The present study has established the phytoconstitution, in vitro antioxidant and antimicrobial activities.