Quantitative Profiling of Seven Steroids in Saliva using LC-MS/MS

Jurgens, Eline; Knaven, Edward; Hegeman, Eva; Gemert, Marjolein van; Emmen, Judith M. A.; Mulder, Yvonne G.; IJsselstijn, Linda; Rooij, Ben de; Noij, Theo

doi:10.17145/jab.19.006

Cited by 4 publications

(7 citation statements)

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“…A selective and sensitive LC-MS/MS method (using multiple reaction monitoring upon electrospray ionisation) was developed for steroids (androstenedione, cortisol, cortisone, 11-deoxycortisol, 21-deoxycortisol, 17α-hydroxyprogesterone, and testosterone) in saliva for the diagnosis and treatment of disorders like congenital adrenal hyperplasia, Cushing’s syndrome or Addison’s disease [ 61 ].…”

Section: Introductionmentioning

confidence: 99%

Qualitative and Quantitative Mass Spectrometry in Salivary Metabolomics and Proteomics

2023

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The metabolomics and proteomics analysis of saliva, an excellent biofluid that is a rich source of biological compounds, allows for the safe and frequent screening of drugs, their metabolites, and molecular biomarkers of various diseases. One of the most frequently used analytical methods in saliva analysis is liquid chromatography coupled with mass spectrometry (LC-MS) and tandem mass spectrometry. The low ionisation efficiency of some compounds and a complex matrix makes their identification by MS difficult. Furthermore, quantitative analysis by LC-MS frequently cannot be performed without isotopically labelled standards, which usually have to be specially synthesised. This review presented reports on qualitative and quantitative approaches in salivary metabolomics and proteomics. The purpose of this manuscript was to present the challenges, advances, and future prospects of mass spectrometry, both in the analysis of salivary metabolites and proteins. The presented review should appeal to those interested in the recent advances and trends in qualitative and quantitative mass spectrometry in salivary metabolomics and proteomics, which may facilitate a diagnostic accuracy, the evaluation of treatment efficacy, the early diagnosis of disease, and a forensic investigation of some unapproved drugs for any medical or dietary administration.

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Section: Introductionmentioning

confidence: 99%

Qualitative and Quantitative Mass Spectrometry in Salivary Metabolomics and Proteomics

2023

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“…A high percentage of published methods rely on reversed-phase chromatographic columns, ,,− ,,,,,− and therefore, this strategy was chosen in this case. As is well-known, retention during reversed-phase separation is usually based mainly on hydrophobic and van der Waals interactions .…”

Section: Resultsmentioning

confidence: 99%

“…For more than half a century, gas chromatography combined with mass spectrometry (GC–MS) has been used for the analysis of steroid substances . GC-based techniques using single MS or tandem mass spectrometry (MS/MS) are available. ,− Approaches to the steroid analysis have been enriched in recent years in particular by the use of liquid chromatography (LC). ,,,− The LC–MS/MS techniques offer several advantages over GC–MS, such as the high-throughput analysis (more suitable for a large set of samples), less time-consuming and generally easier sample preparation (usually, no derivatization is required), or the ability to quantify intact steroid conjugates (sulfates or glucuronides) . These properties make LC–MS/MS more suitable for routine use in clinical laboratories.…”

Section: Introductionmentioning

confidence: 99%

“…These are stationary phases based on hydrocarbon chains of various lengths (mainly C18), ,− phenyl-hexyl or, for example, pentafluorophenyl . Detection is most commonly provided by triple-quadrupole MS, ,,,,− ,− but various hybrid approaches are also available (e.g., linear ion trap with an Orbitrap or triple-quadrupole with a linear ion trap). ,,, NASs are studied by LC–MS/MS in various types of mammalian biological matrices such as plasma or serum, ,,,,,− the cerebrospinal fluid, ,, and brain tissues. ,, However, others can also be used: urine, , saliva, ,, hair, , or nails . LC–MS/MS-based methods have been successfully used to determine selected representatives of androgens, estrogens, progestins, and corticosteroids …”

Section: Introductionmentioning

confidence: 99%

“…42,44,51,57 NASs are studied by LC−MS/MS in various types of mammalian biological matrices such as plasma or serum, 13,22,34,41,43,47−49 the cerebrospinal fluid, 13,31,43 and brain tissues. 47,50,51 However, others can also be used: urine, 52,53 saliva, 42,54,55 hair, 46,56 or nails. 57 LC−MS/MSbased methods have been successfully used to determine selected representatives of androgens, estrogens, progestins, and corticosteroids.…”

Section: Introductionmentioning

confidence: 99%

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Simultaneous Determination of Selected Steroids with Neuroactive Effects in Human Serum by Ultrahigh-Performance Liquid Chromatography–Tandem Mass Spectrometry

Kaleta,

Oklestkova,

Klíčová

et al. 2024

ACS Chem. Neurosci.

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Neuroactive steroids are a group of steroid molecules that are involved in the regulation of functions of the nervous system. The nervous system is not only the site of their action, but their biosynthesis can also occur there. Neuroactive steroid levels depend not only on the physiological state of an individual (person's sex, age, diurnal variation, etc.), but they are also affected by various pathological processes in the nervous system (some neurological and psychiatric diseases or injuries), and new knowledge can be gained by monitoring these processes. The aim of our research was to develop and validate a comprehensive method for the simultaneous determination of selected steroids with neuroactive effects in human serum. The developed method enables high throughput and a sensitive quantitative analysis of nine neuroactive steroid substances (pregnenolone, progesterone, 5α-dihydroprogesterone, allopregnanolone, testosterone, 5α-dihydrotestosterone, androstenedione, dehydroepiandrosterone, and epiandrosterone) in 150 μL of human serum by ultrahigh-performance liquid chromatography with tandem mass spectrometry. The correlation coefficients above 0.999 indicated that the developed analytical procedure was linear in the range of 0.90 nmol/L to 28.46 μmol/L in human serum. The accuracy and precision of the method for all analytes ranged from 83 to 118% and from 0.9 to 14.1%, respectively. This described method could contribute to a deeper understanding of the pathophysiology of various diseases. Similarly, it can also be helpful in the search for new biomarkers and diagnostic options or therapeutic approaches.

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Advancements in the gold standard: Measuring steroid sex hormones by mass spectrometry

Conklin

Knezevic

2020

Clinical Biochemistry

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Quantitative Profiling of Seven Steroids in Saliva using LC-MS/MS

Cited by 4 publications

References 0 publications

Qualitative and Quantitative Mass Spectrometry in Salivary Metabolomics and Proteomics

Qualitative and Quantitative Mass Spectrometry in Salivary Metabolomics and Proteomics

Simultaneous Determination of Selected Steroids with Neuroactive Effects in Human Serum by Ultrahigh-Performance Liquid Chromatography–Tandem Mass Spectrometry

Advancements in the gold standard: Measuring steroid sex hormones by mass spectrometry

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