Quantitative proteomic analysis of deciduous molars during cap to bell transition in miniature pig

Li, Yang; Gong, Yiyi; Wu, Xiaoshan; Wang, Fu; Xie, Yilin; Zhu, Zhenxin; Su, Yingying; Zhang, Chunmei; He, Junqi; Deng, Haiteng; Wang, Songlin

doi:10.1016/j.jprot.2017.10.013

Cited by 9 publications

(3 citation statements)

References 52 publications

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“…The miniature pig has a diphyodont dentition similar to that of humans. Thus, our group established a miniature pig model as a tooth development research platform (Wang et al , , ; Li et al , , ). Studies using large animal models have defined the developmental stages and established gene expression profiles of diphyodont dentition (Weaver et al , ; Tucker & Widowski, ; Buchtova et al , ; Sova et al , ).…”

Section: Introductionmentioning

confidence: 99%

Biomechanical stress regulates mammalian tooth replacement via the integrin β1‐RUNX2‐Wnt pathway

et al. 2019

The EMBO Journal

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Renewal of integumentary organs occurs cyclically throughout an organism's lifetime, but the mechanism that initiates each cycle remains largely unknown. In a miniature pig model of tooth development that resembles tooth development in humans, the permanent tooth did not begin transitioning from the resting to the initiation stage until the deciduous tooth began to erupt. This eruption released the accumulated mechanical stress inside the mandible. Mechanical stress prevented permanent tooth development by regulating expression and activity of the integrin b1-ERK1-RUNX2 axis in the surrounding mesenchyme. We observed similar molecular expression patterns in human tooth germs. Importantly, the release of biomechanical stress induced downregulation of RUNX2-wingless/integrated (Wnt) signaling in the mesenchyme between the deciduous and permanent tooth and upregulation of Wnt signaling in the epithelium of the permanent tooth, triggering initiation of its development. Consequently, our findings identified biomechanical stress-associated Wnt modulation as a critical initiator of organ renewal, possibly shedding light on the mechanisms of integumentary organ regeneration.

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Section: Introductionmentioning

confidence: 99%

Biomechanical stress regulates mammalian tooth replacement via the integrin β1‐RUNX2‐Wnt pathway

et al. 2019

The EMBO Journal

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“…The RNA integrity was assessed using an Agilent Bioanalyzer 2100 (Agilent Technologies, USA), and the RNA with RIN (RNA integrity number) > 8.0 is acceptable for transcriptome library construction. [17, 18]…”

Section: Methodsmentioning

confidence: 99%

Integrated Analysis of LncRNA-mRNA Coexpression in the Extracellular Matrix of Developing Deciduous Teeth in Miniature Pigs

Wang

et al. 2019

BioMed Research International

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Miniature pigs, a valuable alternative model for understanding human tooth development, have deciduous teeth from all four tooth families that are replaced once by permanent molars. The extracellular matrix (ECM) supports cells and maintains the integrity of tooth germs during tooth development. However, details on the role of the ECM in tooth development are poorly understood. Here, we performed long noncoding RNA (lncRNA) and messenger RNA (mRNA) expression profiles in the ECM components of deciduous tooth germs by RNA sequencing in miniature pigs. From the early cap to the late bell stages, we identified 4,562 and 3,238 differentially expressed genes (DEGs) from E40 to E50 and E50 to E60, respectively. In addition, a total of 1,464 differentially expressed lncRNAs from E40 to E50 and 969 differentially expressed lncRNAs from E50 to E60 were obtained. Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway analysis showed that DEGs were enriched significantly for multiple signaling pathways, especially for the ECM pathway. We then outlined the detailed dynamic gene expression profiling of ECM components during deciduous molar development. Comparison of the cap and bell stages revealed that the structure and functions of the ECM dynamically changed. The ECM-related genes, including THBS1, COL4A5, COL4A6, COL1A1, CHAD, TNR, GP1BA, and ITGA3, were significantly changed, and some were shown to enrich during the bell stage development. Finally, we outlined the coexpression of lncRNAs and ECM properties during tooth development. We showed that the interplay of key lncRNAs could change ECM processes and influence the ECM establishment of tooth patterns to accomplish full tooth formation. These results might provide information to elucidate the regulation network of the lncRNA and ECM in tooth development.

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“…Based on the cell-cell interaction strategy, dental epithelial and mesenchymal tissues isolated from mouse embryonic tooth germs can be reconstituted into bioengineered tooth germs, which develop into fully-fledged functional teeth. 75,81,82 In a miniature swine model, the molecular level of epithelium-mesenchyme interactions, [83][84][85][86] cell-ECM interactions, 87 biomechanical stress, 88 and tooth development niches have been well identified. A miniature swine bioengineered tooth bud was generated based on the epithelium-mesenchyme interaction strategy verified after transplantation in mouse subrenal capsules and jawbone, 89 in addition to infusion with bone marrow stem cells and local aspirin administration to remodel immune cells.…”

Section: Stem Cell Niche-functionalized Strategies For Tooth Regenera...mentioning

confidence: 99%

Stem cell niches functionalized strategies for organ regeneration and manufacturing

Liu,

Hu,

Zhang

et al. 2023

TIME

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Organ regeneration and manufacturing are promising new research directions in the life sciences. Stem cells and their niches, which exist in most adult organs, play a central role in organ development, homeostasis, and regeneration. Although considerable advances have been made in stem cell-mediated organ regeneration and manufacturing in recent decades, their clinical effectiveness remains unsatisfactory. Stem cell niches comprise a dynamic microenvironment that supports stem cells throughout their lifetime and are critical to stem cell fate. Based on recent research on stem cell niches, tremendous progress has been made in organ regeneration and manufacturing in vivo and in vitro. In this review, we discuss recent advances in the composition and function of stem cell niches during regeneration. We also discuss stem cell niche remodeling using cell-cell interaction, extracellular matrix (ECM) reconstruction, cell-ECM interaction, and key signaling-based niche strategies to promote endogenous tooth, gastrointestinal tract, and liver regeneration and its application in organoids and organoid-on-chip construction.

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Quantitative proteomic analysis of deciduous molars during cap to bell transition in miniature pig

Cited by 9 publications

References 52 publications

Biomechanical stress regulates mammalian tooth replacement via the integrin β1‐RUNX2‐Wnt pathway

Biomechanical stress regulates mammalian tooth replacement via the integrin β1‐RUNX2‐Wnt pathway

Integrated Analysis of LncRNA-mRNA Coexpression in the Extracellular Matrix of Developing Deciduous Teeth in Miniature Pigs

Stem cell niches functionalized strategies for organ regeneration and manufacturing

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