Quantitative proteomic analysis of okadaic acid treated mouse small intestines reveals differentially expressed proteins involved in diarrhetic shellfish poisoning

Wang, Juan; Wang, Yuyu; Lin, Lin; Gao, Yue; Hong, Huasheng; Wang, Dazhi

doi:10.1016/j.jprot.2012.01.010

Cited by 47 publications

(35 citation statements)

References 62 publications

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“…GAPDH was used as a control in Western blot as did in previous literature (Bovee et al, 2011;Wang et al, 2012). Bovee et al (2011) found that the expression of GAPDH was not affected by any treatments with OA and DTX-1, even not by DTX-1 concentrations of 30 and 100 nmol/L that were cytotoxic for the Caco-2 cells.…”

Section: Protein Validation By Western Blotmentioning

confidence: 86%

“…Bovee et al (2011) found that the expression of GAPDH was not affected by any treatments with OA and DTX-1, even not by DTX-1 concentrations of 30 and 100 nmol/L that were cytotoxic for the Caco-2 cells. Wang et al (2012) used GAPDH as a control for proteomic analysis when they compared protein profiles of mice small intestines after a single oral administration of 750 mg/kg OA. As demonstrated in Fig.…”

Section: Protein Validation By Western Blotmentioning

confidence: 99%

“…In contrast with conventional methods, proteomic technique allows simultaneous isolation and identification of hundreds to thousands of proteins in one sample. So it has extracted much attention, and has been used to identify biomarkers, design novel drug targets, and monitor therapeutic processes (Wang et al, 2012). However, to date just a very few studies have exploited this technique to study the effects of phycotoxins on bivalves (Manfrin et al, 2012).…”

Section: Introductionmentioning

confidence: 99%

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Proteomic profile in Perna viridis after exposed to Prorocentrum lima, a dinoflagellate producing DSP toxins

Huang

Zou

Weng

et al. 2015

Environmental Pollution

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Section: Protein Validation By Western Blotmentioning

confidence: 86%

Section: Protein Validation By Western Blotmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Proteomic profile in Perna viridis after exposed to Prorocentrum lima, a dinoflagellate producing DSP toxins

Huang

Zou

Weng

et al. 2015

Environmental Pollution

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“…Intestine of mice administered with the highest dose of each toxin presented decreased enterocytes microvilli that were not seen after oral DTX2 administration [29]. Ultrastructural evaluation showed mitochondria in various stages of swelling that could be related to alteration of the villin that maintain mitochondrial integrity and also is involved in actin dynamics [34]. RER swelling in liver of mice treated with OA can be due to modifications in water and ions contents as well as to alteration on protein synthesis, translocation and accumulation induced by the toxin [35].…”

Section: Cellular Physiology and Biochemistrymentioning

confidence: 96%

Toxic Action Reevaluation of Okadaic Acid, Dinophysistoxin-1 and Dinophysistoxin-2: Toxicity Equivalency Factors Based on the Oral Toxicity Study

Abal

Louzao

Suzuki

et al. 2018

Cell Physiol Biochem

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Background/Aims: Okadaic acid (OA) and the structurally related compounds dinophysistoxin-1 (DTX1) and dinophysistoxin-2 (DTX2) are marine phycotoxins that cause diarrheic shellfish poisoning (DSP) in humans due to ingestion of contaminated shellfish. In order to guarantee consumer protection, the regulatory authorities have defined the maximum level of DSP toxins as 160 µg OA equivalent kg^-1 shellfish meat. For risk assessment and overall toxicity determination, knowledge of the relative toxicities of each analogue is required. In absence of enough information from human intoxications, oral toxicity in mice is the most reliable data for establishing Toxicity Equivalence Factors (TEFs). Methods: Toxins were administered to mice by gavage, after that the symptomatology and mice mortality was registered over a period of 24 h. Organ damage data were collected at necropsy and transmission electron microscopy (TEM) was used for ultrastructural studies. Toxins in urine, feces and blood were analyzed by HPLC-MS/MS. The evaluation of in vitro potencies of OA, DTX1 and DTX2 was performed by the protein phosphatase 2A (PP2A) inhibition assay. Results: Mice that received DSP toxins by gavage showed diarrhea as the main symptom. Those toxins caused similar gastrointestinal alterations as well as intestine ultrastructural changes. However, DSP toxins did not modify tight junctions to trigger diarrhea. They had different toxicokinetics and toxic potency. The lethal dose 50 (LD₅₀) was 487 µg kg^-1 bw for DTX1, 760 µg kg^-1 bw for OA and 2262 µg kg^-1 bw for DTX2. Therefore, the oral TEF values are: OA = 1, DTX1 = 1.5 and DTX2 = 0.3. Conclusion: This is the first comparative study of DSP toxins performed with accurate well-characterized standards and based on acute toxicity data. Results confirmed that DTX1 is more toxic than OA by oral route while DTX2 is less toxic. Hence, the current TEFs based on intraperitoneal toxicity should be modified. Also, the generally accepted toxic mode of action of this group of toxins needs to be reevaluated.

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“…These and other OMICS studies help to reduce uncertainties associated with the ecological and health risk assessment process by deciphering toxicity mechanisms and modes of action [7][8][9], identifying biomarkers of exposure and toxicity [10], studying toxic effects and environmental diseases [11,12], and facilitating cross-species extrapolation [13,14]. A combination of genomic, proteomic, and bioinformatic approaches was also used to study toxic mechanisms of fungicides [15], and toxicity pathways of endocrine disrupting chemicals with different known or unknown toxic modes of action [16].…”

Section: Introductionmentioning

confidence: 99%

Environmental OMICS: Current Status and Future Directions

Ge¹,

Wang²,

Chiu³

et al. 2013

JIOMICS

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Objectives: Applications of OMICS to high throughput studies of changes of genes, RNAs, proteins, metabolites, and their associated functions in cells or organisms exposed to environmental chemicals has led to the emergence of a very active research field: environmental OM-ICS. This developing field holds an important key for improving the scientific basis for understanding the potential impacts of environmental chemicals on both health and the environment. Here we describe the state of environmental OMICS with an emphasis on its recent accomplishments and its problems and potential solutions to facilitate the incorporation of OMICS into mainstream environmental and health research.Data sources: We reviewed relevant and recently published studies on the applicability and usefulness of OMICS technologies to the identification of toxicity pathways, mechanisms, and biomarkers of environmental chemicals for environmental and health risk monitoring and assessment, including recent presentations and discussions on these issues at The First International Conference on Environmental OMICS (ICEO), held in Guangzhou, China during November 8-12, 2011. This paper summarizes our review.Synthesis: Environmental OMICS aims to take advantage of powerful genomics, transcriptomics, proteomics, and metabolomics tools to identify novel toxicity pathways/signatures/biomarkers so as to better understand toxicity mechanisms/modes of action, to identify/ categorize/prioritize/screen environmental chemicals, and to monitor and predict the risks associated with exposure to environmental chemicals on human health and the environment. To improve the field, some lessons learned from previous studies need to be summarized, a research agenda and guidelines for future studies need to be established, and a focus for the field needs to be developed.Conclusions: OMICS technologies for identification of RNA, protein, and metabolic profiles and endpoints have already significantly improved our understanding of how environmental chemicals affect our ecosystem and human health. OMICS breakthroughs are empowering the fields of environmental toxicology, chemical toxicity characterization, and health risk assessment. However, environmental OMICS is still in the data generation and collection stage. Important data gaps in linking and/or integrating toxicity data with OMICS endpoints/profiles need to be filled to enable understanding of the potential impacts of chemicals on human health and the environment. It is expected that future environmental OMICS will focus more on real environmental issues and challenges such as the characterization of chemical mixture toxicity, the identification of environmental and health biomarkers, and the development of innovative environmental OMICS approaches and assays. These innovative approaches and assays will inform chemical toxicity testing and prediction, ecological and health risk monitoring and assessment, and natural resource utilization in ways that maintain human health and protects the environment in a...

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Quantitative proteomic analysis of okadaic acid treated mouse small intestines reveals differentially expressed proteins involved in diarrhetic shellfish poisoning

Cited by 47 publications

References 62 publications

Proteomic profile in Perna viridis after exposed to Prorocentrum lima, a dinoflagellate producing DSP toxins

Proteomic profile in Perna viridis after exposed to Prorocentrum lima, a dinoflagellate producing DSP toxins

Toxic Action Reevaluation of Okadaic Acid, Dinophysistoxin-1 and Dinophysistoxin-2: Toxicity Equivalency Factors Based on the Oral Toxicity Study

Environmental OMICS: Current Status and Future Directions

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