Quantitative proteomic analysis of Vietnamese krait venoms: Neurotoxins are the major components in Bungarus multicinctus and phospholipases A2 in Bungarus fasciatus

Ziganshin, Rustam H.; Kovalchuk, Sergey I.; Arapidi, Georgij; Starkov, Vladislav G.; Hoang, Anh; Nguyen, Thao; Nguyen, Khoa Cuu; Shoibonov, B. B.; Tsetlin, Victor I.; Utkin, Yuri N.

doi:10.1016/j.toxicon.2015.08.026

Cited by 61 publications

(44 citation statements)

References 19 publications

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“…The abundance of venom proteins and peptides identified in Pakistan RVV by LC-MS/MS analysis was determined by spectral count method that has been used for measuring protein abundance in labelfree shotgun proteomics [20,21]. The relative abundance of a protein in a particular chromatographic fraction was determined by the method described by Tan et al [8] however, with the following modifications (Eq.…”

Section: Identification Of Pakistan Rvv Proteome By Lc-ms/ms Analysismentioning

confidence: 99%

A proteomic analysis of Pakistan Daboia russelii russelii venom and assessment of potency of Indian polyvalent and monovalent antivenom

Mukherjee

Kalita

Mackessy

2016

Journal of Proteomics

144

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Section: Identification Of Pakistan Rvv Proteome By Lc-ms/ms Analysismentioning

confidence: 99%

A proteomic analysis of Pakistan Daboia russelii russelii venom and assessment of potency of Indian polyvalent and monovalent antivenom

Mukherjee

Kalita

Mackessy

2016

Journal of Proteomics

144

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“…However, no cytotoxins or cardiotoxins were encountered in the venom of B. flaviceps , consistent with the results of a recent analysis of B. caeruleus venom, in which these proteins were also not detected [ 16 ]. Interestingly, we were not able to demonstrate the presence of α-bungarotoxin, a 3FTx detected in the venoms of the congeneric species B. candidus , B. fasciatus [ 21 ], and B. multicinctus [ 26 ]. As it was also not detected in the transcriptome study of B. flaviceps [ 25 ], we speculate that its absence could indicate a differential need of suite of toxins for prey taken by B. flaviceps .…”

Section: Resultsmentioning

confidence: 97%

“…For example, Malaysian B. candidus and B. fasciatus venoms revealed relative abundances of 4.9% and 4.7%, respectively [ 21 ]. In addition, AChEs were detected in very low abundances (1.1%) in the Vietnamese B. multicinctus venom [ 26 ]. This might indicate that AChEs play a more important role in envenomation in B. flaviceps than in congeneric venoms.…”

Section: Resultsmentioning

confidence: 99%

Proteomic Deep Mining the Venom of the Red-Headed Krait, Bungarus flaviceps

Chapeaurouge

Silva

Carvalho

et al. 2018

Toxins

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The use of -omics technologies allows for the characterization of snake venom composition at a fast rate and at high levels of detail. In the present study, we investigated the protein content of Red-headed Krait (Bungarus flaviceps) venom. This analysis revealed a high diversity of snake venom protein families, as evidenced by high-throughput mass spectrometric analysis. We found all six venom protein families previously reported in a transcriptome study of the venom gland of B. flaviceps, including phospholipases A2 (PLA2s), Kunitz-type serine proteinase inhibitors (KSPIs), three-finger toxins (3FTxs), cysteine-rich secretory proteins (CRISPs), snaclecs, and natriuretic peptides. A combined approach of automated database searches and de novo sequencing of tandem mass spectra, followed by sequence similarity searches, revealed the presence of 12 additional toxin families. De novo sequencing alone was able to identify 58 additional peptides, and this approach contributed significantly to the comprehensive description of the venom. Abundant protein families comprise 3FTxs (22.3%), KSPIs (19%), acetylcholinesterases (12.6%), PLA2s (11.9%), venom endothelial growth factors (VEGFs, 8.4%), nucleotidases (4.3%), and C-type lectin-like proteins (snaclecs, 3.3%); an additional 11 toxin families are present at significantly lower concentrations, including complement depleting factors, a family not previously detected in Bungarus venoms. The utility of a multifaceted approach toward unraveling the proteome of snake venoms, employed here, allowed detection of even minor venom components. This more in-depth knowledge of the composition of B. flaviceps venom facilitates a better understanding of snake venom molecular evolution, in turn contributing to more effective treatment of krait bites.

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“…As such, venom components are of wide interest to many research groups and have been the subject of extensive research. As about 90% of venom components are of peptide origin, all venom analyses use increasingly fast developing proteomic techniques, including electrophoresis [8][9][10][11], immunodetection techniques [12][13][14][15], different types of chromatography [16][17][18][19] and mass spectrometry [20][21][22][23]. Each proteomic method has specific requirements for both the sample components and the protein concentration range.…”

Section: Introductionmentioning

confidence: 99%

Comparison of Methods for Measuring Protein Concentration in Venom Samples

Bocian

Sławek

Jaromin

et al. 2020

Animals

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Simple Summary: Snake venom is mostly composed of proteins and peptides, which are of interest to many researchers due to their potential pharmacological properties. Due to their biochemical character, these components are analyzed using proteomic techniques such as electrophoresis, chromatography and mass spectrometry. A very important stage of such studies is the measurement of protein concentration in the sample, which is most often performed by colorimetric methods. In the presented article, we used five such techniques on venoms of two snake species, namely Agkistrodon contortrix and Naja ashei. In the case of A. contortrix venom, four methods provide similar concentration values, whereas, in the case of N. ashei, the differences between results are very significant. The source of these differences should probably be seen in the differences in amino acid composition of proteins of these two venoms. With this report, we would like to draw attention to the need to select an appropriate method for measuring the concentration of protein in the venom, especially in the case of Elapid species.Abstract: Snake venom is an extremely interesting natural mixture of proteins and peptides, characterized by both high diversity and high pharmacological potential. Much attention has been paid to the study of venom composition of different species and also detailed analysis of the properties of individual components. Since proteins and peptides are the active ingredients in venom, rapidly developing proteomic techniques are used to analyze them. During such analyses, one of the routine operations is to measure the protein concentration in the sample. The aim of this study was to compare five methods used to measure protein content in venoms of two snake species: the Viperids representative, Agkistrodon contortrix, and the Elapids representative, Naja ashei. The study showed that for A. contortrix venom, the concentration of venom protein measured by four methods is very similar and only the NanoDrop method clearly stands out from the rest. However, in the case of N. ashei venom, each technique yields significantly different results. We hope that this report will help to draw attention to the problem of measuring protein concentration, especially in such a complex mixture as animal venoms.

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Quantitative proteomic analysis of Vietnamese krait venoms: Neurotoxins are the major components in Bungarus multicinctus and phospholipases A2 in Bungarus fasciatus

Cited by 61 publications

References 19 publications

A proteomic analysis of Pakistan Daboia russelii russelii venom and assessment of potency of Indian polyvalent and monovalent antivenom

A proteomic analysis of Pakistan Daboia russelii russelii venom and assessment of potency of Indian polyvalent and monovalent antivenom

Proteomic Deep Mining the Venom of the Red-Headed Krait, Bungarus flaviceps

Comparison of Methods for Measuring Protein Concentration in Venom Samples

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