Quantitative proteomics analysis reveals resistance differences of banana cultivar ‘Brazilian’ to Fusarium oxysporum f. sp. cubense races 1 and 4

Dong, Honghong; Li, Yunfeng; Fan, Huiyun; Zhou, Dengbo; Li, Huaping

doi:10.1016/j.jprot.2019.05.004

Cited by 29 publications

(32 citation statements)

References 83 publications

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“…The expression patterns of proteins related with cell cytoskeleton, natural killer cell mediated cytotoxicity and lipid signaling were different in banana during Foc1 and Foc4 infection, suggesting these proteins participated in mediating different resistance to Foc1 and Foc4 in banana cultivar 'Brazilian' [30]. These studies help us to understand the defense mechnism of banana against TR4.…”

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confidence: 83%

“…Proteomics, complementary to transcriptomics, provides an insight into complex biological processes at the global protein level [25][26][27]. In recent years, large-scale studies of proteomics have focused on dissecting interactions between bananas and Foc [28][29][30]. The proteins related to PR response, cell wall strengthening and antifungal compound synthesis were involved in banana defense to TR4 [29].…”

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confidence: 99%

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Proteomic analysis of banana xylem sap provides insight into resistant mechanisms to Fusarium oxysporum f. sp. cubense Tropical Race 4

Zhang¹,

Liu²,

Li³

et al. 2020

Preprint

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Background Fusarium wilt is a destructive soilborne disease of banana caused by Fusarium oxysporum f. sp. cubense ( Foc ), especially Tropical Race 4 (TR4), which is a xylem-invading fungus. It was evident that xylem sap contained macromolecules, such as proteins, involved in disease-resistance processes. However, there is no research to analyze changes in banana xylem sap proteins response to TR4 to date. Methods To gain an integrated understanding of differential protein expression in banana xylem sap during TR4 infection, we performed a comparative proteomic analysis of xylem sap in resistant ‘Pahang’ and susceptible ‘Brazilian’ bananas inoculated with TR4. Results A total of 1036 proteins were detected in xylem sap of both bananas, among which some proteins are involved in ‘signal transduction’, ‘environmental adaptation’, ‘biosynthesis of secondary metabolites’ and ‘lipid metabolism’, indicating that xylem sap contained defense-related proteins. A number of 129 differential expression proteins (DEPs) were identified in 4 possible pairs between resistant and susceptible tested combinations. Of these DEPs, hypersensitive-induced response protein 1 (HIR1), E3 ubiquitin ligase (E3) might play negative roles in ‘Pahang’ response to TR4 attack, whereas chalcone isomerase (CHI), glycine-rich RNA-binding protein (GRP), carboxylesterase (CXE) and GDSL lipase (GLIP) might play positive roles in ‘Pahang’ defense against TR4 infection. Conclusions Banana xylem sap contained defense-related proteins, among which HIRP1, E3, CHI, GRP, CXE and GLIP involved in banana defense against TR4. To our knowledge, this is first report to analyze changes in banana xylem sap proteins response to TR4, which help us to explore molecular mechanisms of banana resistant to Fusarium wilt.

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confidence: 83%

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confidence: 99%

Proteomic analysis of banana xylem sap provides insight into resistant mechanisms to Fusarium oxysporum f. sp. cubense Tropical Race 4

Zhang¹,

Liu²,

Li³

et al. 2020

Preprint

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“…Besides, Parallel Reaction Monitoring (PRM) was a recently developed methodology in targeted mass spectrometry, which involves in the use of a quadrupole-equipped orbitrap [31] and has been widely used to quantify and detect target proteins [32,33]. Also, PRM has been used to validate the reliability of proteomic data, which provides a reliable guarantee for accurate and reliable proteomics data [34].…”

Section: Embryo Vigor Of Shedding Fruitmentioning

confidence: 99%

Comparative Proteomics Profiling Illuminates the Fruitlet Abscission Mechanism of Sweet Cherry as Induced by Embryo Abortion

Qiu

Zhuang

Yang

et al. 2020

IJMS

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Sweet cherry (Prunus avium L.) is a delicious nutrient-rich fruit widely cultivated in countries such as China, America, Chile, and Italy. However, the yield often drops severely due to the frequently-abnormal fruitlet abscission, and few studies on the metabolism during its ripening process at the proteomic level have been executed so far. To get a better understanding regarding the sweet cherry abscission mechanism, proteomic analysis between the abscising carpopodium and non-abscising carpopodium of sweet cherry was accomplished using a newly developed Liquid chromatography-mass spectrometry/mass spectrometry with Tandem Mass Tag (TMT-LC-MS/MS) methodology. The embryo viability experiments showed that the vigor of the abscission embryos was significantly lower than that of retention embryo. The activity of cell wall degrading enzymes in abscising carpopodium was significantly higher than that in non-abscising carpopodium. The anatomy results suggested that cells in the abscission zone were small and separated. In total, 6280 proteins were identified, among which 5681 were quantified. It has been observed that differentially accumulated proteins (DAPs) influenced several biological functions and various subcellular localizations. The Kyoto Encyclopedia of Genes and Genomes (KEGG) enrichment analysis showed that plenty of metabolic pathways were notably enriched, particularly those involved in phytohormone biosynthesis, cell wall metabolism, and cytoskeletal metabolism, including 1-aminocyclopropane-1-carboxylate oxidase proteins which promote ethylene synthesis, and proteins promoting cell wall degradation, such as endoglucanases, pectinase, and polygalacturonase. Differential expression of proteins concerning phytohormone biosynthesis might activate the shedding regulation signals. Up-regulation of several cell wall degradation-related proteins possibly regulated the shedding of plant organs. Variations of the phytohormone biosynthesis and cell wall degradation-related proteins were explored during the abscission process. Furthermore, changes in cytoskeleton-associated proteins might contribute to the abscission of carpopodium. The current work represented the first study using comparative proteomics between abscising carpopodium and non-abscising carpopodium. These results indicated that embryo abortion might lead to phytohormone synthesis disorder, which effected signal transduction pathways, and hereby controlled genes involved in cell wall degradation and then caused the abscission of fruitlet. Overall, our data may give an intrinsic explanation of the variations in metabolism during the abscission of carpopodium.

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“…Fusarium is a large genus of filamentous fungi; its members often are referred to as the pathogenic fungi of plants, such as Fusarium mangiferae (Usha et al, 2019), Fusarium fujikuroi (Hwang et al, 2013), Fusarium oxysporum (Dong et al, 2019), etc. Furthermore, a correlation was found between the quantity of Fusarium fungi and the textural feature of wheat kernel (Ropelewska et al, 2019).…”

Section: Discussionmentioning

confidence: 99%

Effects of Soil Microbiomes and Enzymatic Activities on Glechoma longituba

Liu¹,

Lin²,

Q³

2020

horts

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Soil microbes and enzymes play important roles in plant growth and metabolism. However, for Glechoma longituba (Nakai) Kupr., an important crop with edible and medicinal uses in China, their effects are not well elucidated. To explore their impacts on plant morphology and bioactive compounds, the plant samples and rhizosphere soil of five different G. longituba populations were collected and investigated in this study. After high-throughput sequencing combined with data analyses, high microbial diversity and richness in the rhizosphere soil of each G. longituba population were observed, and the variations on bacterial and fungal community compositions among these soil samples were also proved. The activities of urease, neutral phosphatase, sucrase, protease, and polyphenol oxidase were significantly different among the rhizosphere soils from different G. longituba populations. Among the major microbial communities and soil enzymes we studied, the genera of Tomentella, Sebacina, Fusarium, Nitrospira, and the activity of soil sucrase were remarkably correlative with both the morphological indices and the contents of bioactive compounds of G. longituba by redundancy analysis. These findings would help guide the scientific plantation of G. longituba to promote its medicinal quality.

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Quantitative proteomics analysis reveals resistance differences of banana cultivar ‘Brazilian’ to Fusarium oxysporum f. sp. cubense races 1 and 4

Cited by 29 publications

References 83 publications

Proteomic analysis of banana xylem sap provides insight into resistant mechanisms to Fusarium oxysporum f. sp. cubense Tropical Race 4

Proteomic analysis of banana xylem sap provides insight into resistant mechanisms to Fusarium oxysporum f. sp. cubense Tropical Race 4

Comparative Proteomics Profiling Illuminates the Fruitlet Abscission Mechanism of Sweet Cherry as Induced by Embryo Abortion

Effects of Soil Microbiomes and Enzymatic Activities on Glechoma longituba

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