Quantitative Proteomics Reveals the Regulatory Networks of Circular RNA BTBD7_hsa_circ_000563 in human coronary artery

Abstract

Background: To investigate the association of BTBD7_hsa_circ_000563 expression in coronary artery segments with atherosclerotic stenosis, and to explore the proteome-wide identification of the BTBD7_hsa_circ_000563-regulated proteins in coronary artery Methods: The coronary artery samples were obtained from two autopsy cases. The epicardial coronary artery of every autopsy was divided into 10 segments, and coronary atherosclerosis grade and extent of the coronary artery segments were analysed by Haematoxylin and Eosin (H&E) staining. The BTBD7_hsa_circ_000563 expression of 8 segments from case 2 was quantified using RT-qPCR analysis. Results: The present study demonstrated that coronary artery segments with severe atherosclerotic stenosis showed extremely low expression of the BTBD7_hsa_circ_000563, compared with normal coronary artery segments. Furthermore, it was predicted that hsa-miR-155-5p, and hsa-miR-130a-3p are targets of the BTBD7_hsa_circ_000563. The results from the present study may laid an epigenetic foundation for studying the underlying mechanisms of the development and progression of coronary artery atherosclerosis. Conclusions: BTBD7_hsa_circ_000563 were involved in atherosclerotic changes in coronary artery segments of human being, and the verification study, mechanism study, and function study are necessary in order for CAD patients to benefit from the personalized medicine in the future.