Quantitative receptor radioautography in the study of receptor-receptor interactions in the nucleus tractus solitarii

Fior-Chadi, Débora Rejane; Fuxé, Kjell

doi:10.1590/s0100-879x1998000200006

Cited by 11 publications

(2 citation statements)

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“…Neuronal colocalization suggests the possible interaction of Ang II with Substance P and further involvement of Ang II in trigeminal neuralgia and nociception [12,42,43,46]. Angiotensin receptor type 1 have been reported in the nucleus tractus solitarri (NTS) of rat brain [19,47], moreover Substance P receptors are also localized in NTS of rat brain [32]. …”

Section: Discussionmentioning

confidence: 99%

Endogenous angiotensinergic system in neurons of rat and human trigeminal ganglia

Imboden

Patil

Nussberger³

et al. 2009

Regulatory Peptides

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To clarify the role of Angiotensin II (Ang II) in the sensory system and especially in the trigeminal ganglia, we studied the expression of angiotensinogen (Ang-N)-, renin-, angiotensin converting enzyme (ACE)- and cathepsin D-mRNA, and the presence of Ang II and substance P in the rat and human trigeminal ganglia. The rat trigeminal ganglia expressed substantial amounts of Ang-N- and ACE mRNA as determined by quantitative real time PCR. Renin mRNA was untraceable in rat samples. Cathepsin D was detected in the rat trigeminal ganglia indicating the possibility of existence of pathways alternative to renin for Ang I formation. In situ hybridization in rat trigeminal ganglia revealed expression of Ang-N mRNA in the cytoplasm of numerous neurons. By using immunocytochemistry, a number of neurons and their processes in both the rat and human trigeminal ganglia were stained for Ang II. Post in situ hybridization immunocytochemistry reveals that in the rat trigeminal ganglia some, but not all Ang-N mRNA-positive neurons marked for Ang II. In some neurons Substance P was found colocalized with Ang II. Angiotensins from rat trigeminal ganglia were quantitated by radioimmunoassay with and without prior separation by high performance liquid chromatography. Immunoreactive angiotensin II (ir-Ang II) was consistently present and the sum of true Ang II (1-8) octapeptide and its specifically measured metabolites were found to account for it. Radioimmunological and immunocytochemical evidence of ir-Ang II in neuronal tissue is compatible with Ang II as a neurotransmitter. In conclusion, these results suggest that Ang II could be produced locally in the neurons of rat trigeminal ganglia. The localization and colocalization of neuronal Ang II with Substance P in the trigeminal ganglia neurons may be the basis for a participation and function of Ang II in the regulation of nociception and migraine pathology.

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Section: Discussionmentioning

confidence: 99%

Endogenous angiotensinergic system in neurons of rat and human trigeminal ganglia

Imboden

Patil

Nussberger³

et al. 2009

Regulatory Peptides

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“…A neuromodulação envolvendo a noradrenalina e o NPY é bem caracterizada nas áreas envolvidas no controle central do sistema cardiovascular (YANG et al, 1994b;FIOR-CHADI & FUXE, 1998). A interação entre os receptores α2-adrenérgicos, da noradrenalina, e o Y1 e Y2 de NPY foi descrito há bastante tempo.…”

Section: Medula Espinhalunclassified

"Efeito modulatório da nicotina sobre a neurotransmissão em núcleos encefálicos responsáveis pelo controle cardiovascular em ratos geneticamente hipertensos e normotensos"

Ferrari¹

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Nicotine is one of the most important agents for cardiovascular diseases in tobacco smoking. This alkaloid acts in the blood stream, but it also crosses the blood-brain-barrier and participate in the regulation of pivotal neurotransmitter systems for the blood pressure control and, eventually, for hypertension development. In this context, nicotine abuse could be very relevant to individuals carrying genetic factors to hypertension. The objectives INTRODUÇÃO

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RAPD based genetic variability among cultivated varieties of Aonla (Indian Gooseberry, Phyllanthus emblica L.)

Chaurasia¹,

Subramaniam²,

Krishna³

et al. 2009

Physiol Mol Biol Plants

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Aonla, the Indian Gooseberry (Phyllanthus emblica) is widely grown in India due to its neutraceutical properties. Investigations on the use of RAPD markers enabled us to estimate genetic variability among commercially cultivated varieties. This study also enabled us to distinguish these varieties using a set of four decamer primers, which was otherwise difficult by using morphological markers. Cluster analysis revealed three different groups of varieties directly associated to their place of origin. RAPD markers were also able to differentiate varieties of same origin or even selection from same parents. This information can be used for identification of varieties and further crop improvement programme.

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Quantitative receptor radioautography in the study of receptor-receptor interactions in the nucleus tractus solitarii

Cited by 11 publications

References 56 publications

Endogenous angiotensinergic system in neurons of rat and human trigeminal ganglia

Endogenous angiotensinergic system in neurons of rat and human trigeminal ganglia

"Efeito modulatório da nicotina sobre a neurotransmissão em núcleos encefálicos responsáveis pelo controle cardiovascular em ratos geneticamente hipertensos e normotensos"

RAPD based genetic variability among cultivated varieties of Aonla (Indian Gooseberry, Phyllanthus emblica L.)

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