Quantitative SERRS immunoassay for the detection of human PSA

Stevenson, Ross; Ingram, Andrew; Leung, Hing Y.; McMillan, Donald C.; Graham, Duncan

doi:10.1039/b902174d

Cited by 50 publications

(39 citation statements)

References 24 publications

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“…[76] SERS can be used to enhance native Raman signals, by close proximity to roughened metal surfaces. However, perhaps the more promising application is to probe for specific interactions, for example by detection of specific antigens [37] or enzyme activity [18,77]. Kneipp et al[78] demonstrated the use of SERS to enhance Raman signals in single cells, therefore decreasing the acquisition time for a single map significantly.…”

Section: An Alternative Technique -Surface Enhanced Raman Spectroscopmentioning

confidence: 99%

“…In particular, the technique of surface enhanced Raman spectroscopy (SERS) has been used extensively in assay development in other biomedical research areas for many applications, including ELISA type immunoassays. [37] It has many benefits over standard fluorescence based assays, including lack of photobleaching, multiplexing ability [38] and often increased sensitivity. SERS has also been applied to in vitro cellular assays, to analyse, for example, enzyme activity.…”

Section: Current High Throughput Screening Techniques: Could Vibratiomentioning

confidence: 99%

“…[38] Nanoparticles have been functionalised with antibodies designed to detect specific antigen markers in immunoassays based on both SERS and SERRS techniques. [37,[80][81][82] SERRS based assays have also been used to detect enzyme activity. Moore et al [77] designed an assay to detect a variety of enzyme activities including from lipases, esterases and proteases.…”

Section: An Alternative Technique -Surface Enhanced Raman Spectroscopmentioning

confidence: 99%

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Vibrational spectroscopy as a tool for studying drug-cell interaction: Could high throughput vibrational spectroscopic screening improve drug development?

Jamieson

Byrne²

2017

Vibrational Spectroscopy

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. (2017). Vibrational spectroscopy as a tool for studying drug-cell interaction: could high throughput vibrational spectroscopic screening improve drug development? Vibrational Spectroscopy, vol. 91, July, pp. 16-30. doi.org/10.1016/ j.vibspec.2016 Vibrational spectroscopy as a tool for studying drug-cell interaction: could high throughput vibrational spectroscopic screening improve drug development? AbstractVibrational spectroscopy is currently widely explored as a tool in biomedical applications. An area at the forefront of this field is the use of vibrational spectroscopy for disease diagnosis, ultimately aiming towards spectral pathology. However, while this field shows promising results, moving this technique into the clinic faces the challenges of widespread clinical trials and legislative approval. While spectral pathology has received a lot of attention, there are many other biomedical applications of vibrational spectroscopy, which could potentially be translated to applications with greater ease. A particularly promising application is the use of vibrational spectroscopic techniques to study the interaction of drugs with cells. Many studies have demonstrated the ability to detect biochemical changes in cells in response to drug application, using both infrared and Raman spectroscopy. This has shown potential for use in high throughput screening (HTS) applications, for screening of efficacy and mode of action of potential drug candidates, to speed up the drug discovery process. HTS is still a relatively new and growing area of research and, therefore, there is more potential for new techniques to move into and shape this field. Vibrational spectroscopic techniques come with many benefits over the techniques used currently in HTS, primarily based on fluorescence assays to detect specific binding interactions or phenotypes. They are label free, and an infrared or Raman spectrum provides a wealth of biochemical information, and therefore could reveal not only information about a specific interaction, but about how the overall biochemistry of a cell changes in response to application of a drug candidate. Therefore, drug mode of action could be elucidated. This review will investigate the potential for vibrational spectroscopy, particularly FTIR and Raman spectroscopy, to benefit the field of HTS and improve the drug development process. In addition to FTIR and Raman spectroscopy, surface enhanced Raman spectroscopy (SERS), coherent anti-Stokes Raman spectroscopy (CARS) and stimulated Raman spectroscopy (SRS), will be investigated as an alternative tool in the HTS process.

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Section: An Alternative Technique -Surface Enhanced Raman Spectroscopmentioning

confidence: 99%

Section: Current High Throughput Screening Techniques: Could Vibratiomentioning

confidence: 99%

Section: An Alternative Technique -Surface Enhanced Raman Spectroscopmentioning

confidence: 99%

See 1 more Smart Citation

Vibrational spectroscopy as a tool for studying drug-cell interaction: Could high throughput vibrational spectroscopic screening improve drug development?

Jamieson

Byrne²

2017

Vibrational Spectroscopy

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“…Some types of immunoassay for PSA have been reported, such as fluorescence microscopy, surface plasmon resonance technology, lateral flow immunoassay, enzyme-linked immunosorbent assay (ELISA), radioimmunoassay, immunochromatographic assay system, and SERS based on immunoassays. [15][16][17]26,[37][38][39][40][41][42][43][44] In this study, we applied a sandwich type SERS immunoassay for the detection of PSA with high sensitivity. It shows the possible application of SERS immunoassay with monoclonal antibodies that is sensitive enough for analysis of PSA in human blood samples or cancer cells.…”

Section: Introductionmentioning

confidence: 99%

“…28 Especially, SERS immunoassays based on antigen-antibody binding have been reported for biomarkers for early cancer diagnosis. [12][13][14][15][16][17][18][21][22][23][24]26,30 For successful application of SERS immunoassays for biological sensors, people usually used novel metal nanoparticles modified to integrate bioselective species (e.g., antibodies) with molecular labels for the generation of intense, biolyte-selective SERS responses in immunoassays and other bioanalytical applications.…”

Section: Introductionmentioning

confidence: 99%

Bioanalytical Application of SERS Immunoassay for Detection of Prostate-Specific Antigen

Yoon¹,

Seo²,

Hwang³

et al. 2010

Bulletin of the Korean Chemical Society

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We demonstrate the possible application of the sandwich type surface-enhanced Raman scattering (SERS) immunoassay using antigen-antibody binding for detection of prostate-specific antigen (PSA) in cancer cells. In this sandwich type of SERS immunoassay, to capture antigens onto the immobilized layer of antibodies on the gold substrate we prepared the monolayer of gold nanoparticles on the APTMS-derivatized surface of a glass slide by using the SAM technique. This sandwich type of SERS immunoassay in which antigens on the substrate specifically capture antibodies on a Raman reporter (DSNB coated gold nanoparticles with R6G) could successfully detect PSA at low levels. A strong SERS spectrum of Raman reporter was observed only with a substrate in which PSA is present.

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Surface‐enhanced Raman Spectroscopy (SERS): Protein Application

Chen

Cai

Ruan

et al. 2000

Encyclopedia of Analytical Chemistry

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This article outlines the recent progress in surface‐enhanced Raman spectroscopy (SERS)‐based biological applications, especially in the study of proteins. SERS is a specific Raman spectroscopic technique that provides enhanced Raman signals (several orders of magnitude greater than normal) for numerous Raman‐active analyte molecules adsorbed onto rough metal surfaces. SERS is a sensitive, selective, and versatile technique, and it lends itself readily to fast data acquisition. Therefore, SERS has undergone rapid development because of numerous technical advances in both instrumentation and methods of data analysis and a vast proliferation and combination of basic techniques for its multiple biological applications. This article highlights several representative areas in biology where SERS could be employed. Some of the biological applications of SERS are more developed, whereas others are in their initial stages of development (in laboratories). This article discusses the recent developments in SERS‐based quantitative analysis: (i) directly with different substrates (e.g. biomolecules on electrodes, colloidal particles, and periodic pattern structure and tip‐based substrates) and (ii) indirectly with different types of sensors (e.g. immunogold‐, enzymatic‐, reagent‐, and Raman‐active nanomaterial‐based sensors). Furthermore, SERS‐based techniques are advantageous for obtaining valuable information on protein–protein, protein–ligand, and protein–drug recognitions via spectral differences among molecular bridges. SERS‐based techniques show considerable promise for qualitative and/or quantitative analyses of biological systems.

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Quantitative SERRS immunoassay for the detection of human PSA

Cited by 50 publications

References 24 publications

Vibrational spectroscopy as a tool for studying drug-cell interaction: Could high throughput vibrational spectroscopic screening improve drug development?

Vibrational spectroscopy as a tool for studying drug-cell interaction: Could high throughput vibrational spectroscopic screening improve drug development?

Bioanalytical Application of SERS Immunoassay for Detection of Prostate-Specific Antigen

Surface‐enhanced Raman Spectroscopy (SERS): Protein Application

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