Quantitative Simultaneous Determination of Amlodipine, Valsartan, and Hydrochlorothiazide in “Exforge Hct” Tablets Using High-Performance Liquid Chromatography and High-Performance Thin-Layer Chromatography

Varghese, Susheel John; Ravi, T. K.

doi:10.1080/10826076.2011.565539

Cited by 23 publications

(7 citation statements)

References 26 publications

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“…Numerous analytical techniques have been established for the determination of CEL and AMLO alone or combined with other drugs as spectrophotometric [8][9][10][11], spectrofluorometric [12][13][14][15], high-performance thin-layer chromatography (HPTLC) [16][17][18][19][20][21][22][23][24][25][26][27][28][29][30], HPLC [31][32][33], and electrochemical methods [34,35]. A few recently analytical techniques have been established for the simultaneous estimation of CEL and AMLO such as spectrophotometric [36], spectrofluorimetric [37], and HPLC [38,39].…”

Section: Introductionmentioning

confidence: 99%

Sensitive and validated TLC densitometry method coupled with fluorescence detection for quantitative determination of the newly co-formulated drugs, celecoxib and amlodipine besylate in tablet dosage form

Rizk

Toubar

Ramzy

et al. 2021

AChrom

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New, sensitive, rapid, cost-effective, and validated stability-indicating thin layer chromatographic (TLC) method coupled with fluorescence (FL) detection was developed for the quantitative analysis of celecoxib (CEL) and amlodipine besylate (AMLO) in their laboratory prepared binary mixture using the non-fluorescent TLC silica gel 60 plates. Ethyl acetate: diethylamine: 1-propanol (9:1:0.2, V/V) was used as a developing system. The retention factor (Rf) for each drug was 0.80 ± 0.03 and 0.44 ± 0.01 for CEL and AMLO, respectively. The plates were excited at 264 nm for the simultaneous FL measurement of CEL and AMLO, the calibration curves were linear over a concentration ranges of 30.0–300.0 ng/band and 15.0–150.0 ng/band with mean percentage recoveries of 99.80 ± 0.85 and 99.80 ± 0.77 For CEL and AMLO, respectively. The developed method was applied for the stability studies of the cited drugs in their laboratory prepared binary mixture and the forced degradation products were determined when present in presence of the pure drugs so the method can be considered as a stability-indicating one and it was validated as per ICH guidelines and proved to be accurate and precise.

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Section: Introductionmentioning

confidence: 99%

Sensitive and validated TLC densitometry method coupled with fluorescence detection for quantitative determination of the newly co-formulated drugs, celecoxib and amlodipine besylate in tablet dosage form

Rizk

Toubar

Ramzy

et al. 2021

AChrom

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“…The literature survey reveals that several methods were reported for the individual estimation of VAL and AMP. The simultaneous quantitative determination of both drugs at the same time in pharmaceutical tablets using various methods including HPLC and first derivative of the ratio spectrophotometric (Kul et al, 2010 ;Varghese, 2011), HPLC and Thin layer Chromatography (Varghese and Ravi, 2011), spectrophotometric (Chitlange et al, 2008;Mohammed 2011), HPLC-MS (Lena et al, 2007), TLC (Dhaneshwar , 2009) have been described for many mixtures. Working two forms of active ingredient are shown in Figure 1.…”

Section: Introductionmentioning

confidence: 99%

Simultaneous Spectrophotometric Determination of Hypertension Drug's in Commercial Pharmaceutical by Chemometric Methods

2020

JSTR

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In this study, chemometric approaches using UV spectrophotometry method developed models for simultaneous determination of valsartan (VAL) and amlodipine (AMP) in drug samples. It was used to calculate calibration mixtures between 232 and 254 nm wavelengths at 2 nm intervals for Val and AML spectra at various concentrations. For chemometric analysis of data, the least squares calibration method and basic component regression were used, and the parameters of chemometric procedures were optimized. The analytical performances of this chemometric method were compared by characterizing the sum of residual error squares (PRESS), estimated standard error (SEP), and recoveries (%). A number of synthetic mixtures containing different concentrations of VAL and AML were studied to control the predictive ability of Applied chemometric methods. This method was successfully applied to the actual samples, not affected by the auxiliaries as indicated in the recovery study results. The results obtained in this review encourage these chemometric methods to implement these strategies for standard research and quality control of the two active ingredients.

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“…There are reported methods for the determination of AML, VAL, or HCT in different dosage forms [2,4,[16][17][18][19] and in their binary mixtures [20][21][22]. Few methods were reported for the simultaneous estimation of AML, VAL, and HCT in their ternary mixture [23][24][25][26] and only one spectrophotometric method was developed for the determination of this mixture [27]. In previous work, the authors developed derivative and chemometric methods for the analysis of the same mixture [28].…”

Section: Introductionmentioning

confidence: 99%

Sequential Spectrophotometric Method for the Simultaneous Determination of Amlodipine, Valsartan, and Hydrochlorothiazide in Coformulated Tablets

Darwish

Hassan

Salem

et al. 2013

International Journal of Spectroscopy

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A new, simple and specific spectrophotometric method was developed and validated in accordance with ICH guidelines for the simultaneous estimation of Amlodipine (AML), Valsartan (VAL), and Hydrochlorothiazide (HCT) in their ternary mixture. In this method three techniques were used, namely, direct spectrophotometry, ratio subtraction, and isoabsorptive point. Amlodipine (AML) was first determined by direct spectrophotometry and then ratio subtraction was applied to remove the AML spectrum from the mixture spectrum. Hydrochlorothiazide (HCT) could then be determined directly without interference from Valsartan (VAL) which could be determined using the isoabsorptive point theory. The calibration curve is linear over the concentration ranges of 4–32, 4–44 and 6–20 μg/mL for AML, VAL, and HCT, respectively. This method was tested by analyzing synthetic mixtures of the above drugs and was successfully applied to commercial pharmaceutical preparation of the drugs, where the standard deviation is <2 in the assay of raw materials and tablets. The method was validated according to the ICH guidelines and accuracy, precision, repeatability, and robustness were found to be within the acceptable limits.

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Quantitative Simultaneous Determination of Amlodipine, Valsartan, and Hydrochlorothiazide in “Exforge Hct” Tablets Using High-Performance Liquid Chromatography and High-Performance Thin-Layer Chromatography

Cited by 23 publications

References 26 publications

Sensitive and validated TLC densitometry method coupled with fluorescence detection for quantitative determination of the newly co-formulated drugs, celecoxib and amlodipine besylate in tablet dosage form

Sensitive and validated TLC densitometry method coupled with fluorescence detection for quantitative determination of the newly co-formulated drugs, celecoxib and amlodipine besylate in tablet dosage form

Simultaneous Spectrophotometric Determination of Hypertension Drug's in Commercial Pharmaceutical by Chemometric Methods

Sequential Spectrophotometric Method for the Simultaneous Determination of Amlodipine, Valsartan, and Hydrochlorothiazide in Coformulated Tablets

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