Quantitative studies of lymphoid organs, blood and lymph in inbred athymic and euthymic LEW rats under germfree and specified-pathogen-free conditions

Klausen, Bjarne; Hougen, Hans Petter

doi:10.1258/002367787781363318

Cited by 11 publications

(12 citation statements)

References 11 publications

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“…Germ-free rats and mice have lower circulating leukocytes, below the historical control range, than their specific-pathogen free (SPF) counterparts in the same laboratory. In addition, germ-free rodents have lower cellularity in the thymus, lymph nodes, spleen and/or bone marrow than their SPF counterparts (5,14,16,19,33).…”

Section: Discussionmentioning

confidence: 99%

Safety of Aquaflor (Florfenicol, 50% Type A Medicated Article), Administered in Feed to Channel Catfish, Ictalurus punctatus

Gaikowski

Wolf

Endris³

et al. 2003

Toxicol Pathol

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Aquaflor, a feed premix containing the broad spectrum antibacterial agent florfenicol (50% w/w), is being developed for use to control enteric septicemia (ESC) in channel catfish Ictalurus punctatus caused by the gram-negative enterobacterium Edwardsiella ictaluri. The recommended dose of Aquaflor to control ESC is 10 mg/kg body weight (BW)/day for 10 days. The study objective was to determine the safety of Aquaflor administered in feed to channel catfish at doses of 0 (control), 10, 30, and 50 mg/kg BW/day for 20 consecutive days. Parameters evaluated included daily mortality, behavioral (appetite, distribution, flight/fright response), and water chemistry observations, initial and terminal weight measurements, and gross and microscopic pathology. Medicated feed consumption was 67-86% of target with group mean doses of 8.5 mg/kg BW/day, 24.6 mg/kg BW/day, and 34.9 mg/kg BW/day. There were no mortalities or clinically observable changes noted at any of the dose levels tested. Aquaflor-related changes were limited to the food consumption and histopathology data. Although Aquaflor-related decreased feed consumption was noted in the 30 and 50 mg/kg BW/day groups, there were no differences in fish growth among the treatment groups. Aquaflor-related histopathology findings were limited to a histomorphologically evident dose-dependent decrease in hematopoietic/lymphopoietic tissue in the anterior kidneys, posterior kidneys, and spleens of channel catfish.

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Section: Discussionmentioning

confidence: 99%

Safety of Aquaflor (Florfenicol, 50% Type A Medicated Article), Administered in Feed to Channel Catfish, Ictalurus punctatus

Gaikowski

Wolf

Endris³

et al. 2003

Toxicol Pathol

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“…Finally, the mal for such studies, since the structare of existence of inbred immunodeficient rat the periodontal tissues in rat molar regions is strains makes the rat model attractive for very sitnilar to that in man (6). Furthermore, studies of host reactions in periodontal disrats can easily be kept under germfree condi-ease (9).…”

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confidence: 99%

Two complementary methods of assessing periodontal bone level in rats

Klausen

Evans

Sfintescu

1989

European J Oral Sciences

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Kiausen B^ Evans RT, Sfintescu C; Two eomplenientary methods of assessing periodontal bone level in rats. Scand J Dent Res 1989; 97: +94-9.Abstract -SeveraJ methods have been applied to measure periodontal disease in rats. The purpose of the present study was to test the reproducibility of a morphometric and a radiographic method and to describe tiie correlation between the two methods. Periodontal bone loss on 25 defleshed rat heads was assessed under microseope by measuring the distances from the cementoenamel junctiots tO' the alveolar bone crest at 36 buccal sites in each animal. On magnified radiographs from 25 rat mandibles periodontal bone support was expressed by the ratio apes-deepest bony defect: apex-ctisp tip distally on first molars. .All measurements were performed blind and in duplicate on two separate occasions. The bilateral 95% confidence limits for the error of method of measurement were estimated from the /-distribution. In a second experiment 50 rat heads were assessed by both methods, and the correlation between the recordings was estimated by the Spearman rank correlation analysis. Compared to the considerable total variation in the material, tbe variations due to error of methods of measurement were small^ i.e. the reproducibility of both methods was satisfactory. A significant correlation was found between tbe methods. Since the morpbometric method mainly measures horizontal bone loss, whereas the radiographic method detects intrabony interproximal defects, it is concluded that future studies would benefit from applying both methods to assess alveolar bone loss in rats.

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“…The general protein was determined using the biurettic method according to the instructions for the diagnostic set [11][12][13][14][15][16][17]. In order to carry out quantitative analysis, finished reagents and test samples were applied according to the recommended scheme.…”

Section: Methodsmentioning

confidence: 99%

“…Cholesterol level was controlled using enzymatic colorimetric method in accordance with instructions [11][12][13][14][15][16][17]. Ready reagents and test samples were entered in accordance with the schematic in the instructions during the experiment.…”

Section: Methodsmentioning

confidence: 99%

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Study of the impact of the food biopolymer system on the clinical-biochemical status of white inbred mice in the invivo experiment

et al. 2021

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Studies have been conducted on the effect of the food biopolymer composition on the clinical-biochemical status of white inbred mice. The experiment showed that the development of laboratory animals was carried out without abnormalities with the complete safety of all individuals. Clinical-biochemical dynamics, glucose monitoring and the results of experiments in the exchange of macro and microelements of plasma of mouse blood indicated the normal flow of metabolic processes. In addition, the animals that consumed food additives had a higher rate of absorption of feed carbohydrates and a predominance of osteosynthesis processes in animal bone tissue. The studies conducted allow us to conclude that enrichment and trace elements increase the availability of trace elements, as well as activate the formation and strengthening of the bone tissue of the body.

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Quantitative studies of lymphoid organs, blood and lymph in inbred athymic and euthymic LEW rats under germfree and specified-pathogen-free conditions

Cited by 11 publications

References 11 publications

Safety of Aquaflor (Florfenicol, 50% Type A Medicated Article), Administered in Feed to Channel Catfish, Ictalurus punctatus

Safety of Aquaflor (Florfenicol, 50% Type A Medicated Article), Administered in Feed to Channel Catfish, Ictalurus punctatus

Two complementary methods of assessing periodontal bone level in rats

Study of the impact of the food biopolymer system on the clinical-biochemical status of white inbred mice in the invivo experiment

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