Quantitative Study of the Myenteric Plexus of the Descending Colon of Young Rats Subjected to Intense Protein Deficiency

Araújo, Eduardo José de Almeida; Sant’Ana, Débora de Mello Gonçales; Molinari, Sônia Lucy; Neto, Marcílio Hübner de Miranda

doi:10.4067/s0717-95022006000500013

Cited by 12 publications

(18 citation statements)

References 31 publications

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“…Body weight reduction was probably a result of the adaptation to the protein malnutrition due to lower nutrient availability, as well as lower development of fat and lean mass in these animals. such adaptation possibly demonstrates a mean of maintaining the metabolism of the noble tissues already constituted (such as the nervous tissue) in order to keep them alive longer so that the animal is able to find a new source of food fulfilling the demand of its organism 19 .…”

Section: Discussionmentioning

confidence: 99%

“…while studying the protein availability reduction to 8% for rats during pregnancy, lactation, and weaning until their 60-day-old, it was observed a 45.21% reduction on the ileum length and wall thickness 6 . The small size of the organ may be considered as an adaptive response either to the decreased amount of chow or the reduced metabolic rate 19 . The reduction of the organs is believed to be a reflex of its own tissue alterations as they may present different degrees of commitment according to their own structural and cellular organization 6 .…”

Section: Discussionmentioning

confidence: 99%

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Quantitative analysis of the neurons from the myenteric plexus in the ileum of rats submitted to severe protein deficiency

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Hermes

Almeida

et al. 2008

Arq. Neuro-Psiquiatr.

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-The effects of protein malnutrition on the quantitative aspects of the myenteric plexus in the ileum of adult Rattus norvegicus were assessed. Thirty 90-day-old rats were divided into two groups: Control Group (CG, n=15) and Experimental Group (EG, n=15). The CG received 26% protein chow and the EG received 4% protein chow for 90 days. At the end of the experiment, the animals from the CG weighed 369.63±26.33, and the ones from the EG 215.34±56.31. The ileum was submitted to Giemsa, NADH-and NADPH-diaphorase technique in order to evidence nervous cells in the whole-mount preparations. Animals from the EG presented a 41.75% body weight loss in relation to the CG as well as 17.6% length reduction for the ileum-jejunum. Moreover, the organ was 41% lighter for the EG. Giemsa-stained neurons were 17.02% more concentrated in the EG (p>0.05). NADH-diaphorase-stained neurons were 26.6% more concentrated in the EG (p<0.05), while the NADPH-diaphorase were 26.28% more concentrated in this group (p<0.05).KEy worDs: protein malnutrition, rat ileum, enteric neurons. Análise quantitativa dos neurônios do plexo mientérico do íleo de ratos submetidos a intensa carência de proteínasResumo -Avaliou-se o efeito da desnutrição protéica sobre o número de neurônios mientéricos do íleo de ratos adultos. Foram utilizados 30 animais (90 dias de idade), divididos em dois grupos: controle (GC, n=15) e experimental (GE, n=15), sendo oferecido ao GC ração com teor protéico de 26% e, para o GE, ração com 4% de proteína, durante 90 dias. os animais do grupo controle pesaram 369,63±26,33g e o experimental 215,34±56,31g. Preparados de membrana do íleo foram submetidos à técnica de Giemsa, NADH-e NADPH-diaforase. os animais do GE apresentaram perda de peso de 41,75%, em relação ao GC e redução do comprimento do jejuno-íleo de 17,6%, além disso, o órgão apresentou-se 41% mais leve no GE. os neurônios corados com a técnica de Giemsa apresentaram-se 17,02% mais concentrados no GE (p>0,05). os neurônios NADH-diaforase apresentaram-se 26,60% mais concentrados no GE (p<0,05). E os neurônios NADPH-diaforase apresentaramse 26,28% mais concentrados neste grupo (p<0,05).PAlAvrAs-CHAvE: má nutrição protéica, íleo, rato, neurônio entérico.Paper presented as part of the first author´s graduation monograph to the UNIPAr Nursing Departament; Experimental Neurogastroenterology laboratory -UNIPAr; Umuarama, Pr Brazil. Financial support: UNIPAr.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Quantitative analysis of the neurons from the myenteric plexus in the ileum of rats submitted to severe protein deficiency

Moreira

Hermes

Almeida

et al. 2008

Arq. Neuro-Psiquiatr.

Self Cite

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“…Changes in neurons of the myenteric plexus have been described in different conditions and experimental models which have tried to elucidate the mechanisms that lead to functional impairment of the digestive tube (Fregonesi et al 2001, Furlan et al 2002, Zanoni et al 2003, Fontes et al 2004, Alves et al 2006, Araújo et al 2006, Silverio et al 2008, Silva et al 2008.…”

Section: Introductionmentioning

confidence: 99%

Effects of ascorbic acid supplementation in ileum myenteric neurons of streptozotocin-induced diabetic rats

et al. 2009

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295Pesq. Vet. Bras. 29(4): 295-302, abril 2009 RESUMO.-[Efeitos da suplementação do ácido ascórbico nos neurônios mientéricos do íleo de ratos diabéticos induzidos por estreptozootocina.] A exacerbação do estresse oxidativo e da via do poliol que comprometem o plexo mioentérico são alterações metabóli-cas características do diabetes. O ácido ascórbico (AA) é antioxidante e inibidor da aldose redutase, podendo atuar como neuroprotetor. Verificaram-se os efeitos da suplementação com AA sobre o número e a área do perfil do corpo celular (PC) de neurônios mioentéricos em ratos diabéticos induzidos por estreptozootocina. Formaram-se quatro grupos com cinco animais cada: normoglicêmico (C); diabético (D); diabético tratado com AA (DS); e normoglicêmico tratado com AA (CS). Três vezes por semana administrou-se 50mg de AA para cada animal (grupos DS e CS). Após 90 dias e eutanásia com tiopental, o íleo foi coletado e processado para a técnica da NADPHdiaforase. Não se observaram diferenças (P>0,05) na densidade neuronal entre os grupos. A área do PC foi menor (P<0,05) para os grupos DS e CS, com incidência maior de neurônios com área do PC superior a 200μm 2 para os grupos C e D. Concluiu-se que o AA não influenciou a densidade neuronal do íleo, mas foi neuroprotetor The exacerbation of the oxidative stress and of the polyol pathway which impair damage myenteric plexus are metabolic characteristics of diabetes. The ascorbic acid (AA) is an antioxidant and an aldose reductase inhibitor, which may act as neuroprotector. The effects of AA supplementation on the density and cellular body profile area (CP) of myenteric neurons in STZ-induced diabetes in rats were assessed. Four groups with five animals each were formed: normoglycemic (C); diabetic (D); AA-treated diabetic (DS) and AA-treated normoglycemic (CS). Dosagen of 50mg of AA were given, three times a week, for each animal (group DS and CS). Ninety days later and after euthanasia, the ileum was collected and processed for the NADPH-diaphorase technique. There were no differences (P>0.05) in the neuronal density among the groups. The CP area was lower (P<0.05) in the DS and CS groups, with a higher incidence of neurons with a CP area exceeding 200μm 2 for groups C and D. The AA had no influence on the neuronal density in the ileum but had a neuroprotective effect, preventing the increase in the CP area and allowing a higher number of neurons with a CP area with less than 200μm 2 .INDEX TERMS: Vitamin C, diabetes, intestine, myenteric plexus.

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“…It is a highly applied method for studying intramural plexi as it ensures a broader and safer analysis of what was collected from a histological section (Gabella 1979, Natali and Miranda-Neto 1996, Araújo et al 2006, Natali et al 2005. We also chose to carry out the quantifications and measurements by considering the regionalization of the intestinal circumpherence (Sant`Ana et al 1997) granting more homogeneity to the sample.…”

Section: Myenteric Neurons In Malnourished Ratsmentioning

confidence: 99%

Characterization of the myenteric neuronal population and subpopulation of the duodenum of adult wistar rat fed with hypoproteic chow

Sant’Ana

Araújo

Ramos

et al. 2012

An. Acad. Bras. Ciênc.

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The effects of severe protein malnutrition (4%) on myenteric neurons of Wistar rat duodenum, in relation to a standard 22%-protein diet for rodents, were assessed in this study. Segments of the duodenum from 10 rats from each nutritional group were submitted to the elaboration of whole mounts -5 stained with Giemsa to determine the total population of myenteric neurons and the others stained by a histochemical method to detect nervous cells through the NADPH-diaphorase enzyme activity for studying the subpopulation of nitrergic neurons. The area of 100 neurons per animal, totalizing 2,000 neurons, were randomly measured by using the Image Pro-Plus ® software. Malnourished rats presented 34.38% lower body weight and 10.60% duodenum length reduction when compared to the control group. Quantitative analysis demonstrated no significant differences between control and malnourished group by using Giemsa; however, as the organ reduction was not followed by an increase inversely proportional to the density of neurons, the condition imposed suggests the loss of neurons from the total population. Nevertheless, through NADPH-d histochemistry, there was a neuronal density increase for the malnourished group. There was no significant difference between the groups for both techniques with respect to the morphometric analysis of the body cell.

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Quantitative Study of the Myenteric Plexus of the Descending Colon of Young Rats Subjected to Intense Protein Deficiency

Cited by 12 publications

References 31 publications

Quantitative analysis of the neurons from the myenteric plexus in the ileum of rats submitted to severe protein deficiency

Quantitative analysis of the neurons from the myenteric plexus in the ileum of rats submitted to severe protein deficiency

Effects of ascorbic acid supplementation in ileum myenteric neurons of streptozotocin-induced diabetic rats

Characterization of the myenteric neuronal population and subpopulation of the duodenum of adult wistar rat fed with hypoproteic chow

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