Quantitative trait loci associated with soybean tolerance to low phosphorus stress based on flower and pod abscission

Abstract

Low phosphorus (P) stress limits soybean production. A population of 152 recombinant inbred lines (RILs) derived from a cross between ÔBogaoÕ (P sensitive variety) and ÔNannong 94-156Õ (P tolerant variety) and 248 markers were used to map quantitative trait loci (QTLs) for low-P tolerance. Two pot culture trials were conducted and low-P tolerance evaluated using flower and pod abscission rate under low P and normal P. Conditional QTLs and epistasis for tolerance to low P were also analysed. A conditional QTL (near Satt274) on linkage group D1b+W was identified which conferred low-P tolerance epistatic effects and coincided with previously discovered QTLs. An additive QTL, qFARLPG-07, for flower abscission rate under low P was detected with a LOD score of 7.79 and explained 32.3% of phenotypic variation. It was detected at the same interval of the corresponding QTL for other traits across years. This region coincided with two conditional QTLs (cqFARLPG-07 and cqPARLPG-07), from the P-tolerant parent ÔNannong 94-156Õ related to low-P tolerance. These results will provide a basis for further fine mapping and eventual cloning of the P-efficiency genes in soybean.

Mineralogy and Maximum Phosphorus Adsorption Capacity in Soybean Development

“…This is a 70% increase due to the P fertilization. This way, in agreement to other investigations (Zhang et al, 2010;Cruz, Souza Filho, & Pelacani, 2015), soils with low P availability provide lower number of flowers and a higher pod abortion, compromising productivity. In this sense, Batistella Filho, Ferreira, Vieira, and Cruz (2013) also obtained a linear response for soybean pod production in Typic Haplortox up to the maximum test dose of 160 kg ha -1 of P 2 O 5 .…”

Transcriptome Dataset of Soybean (Glycine max) Grown under Phosphorus-Deficient and -Sufficient Conditions

“…Soybean cultivar, NN94-156 (B20), is a low-P tolerance soybean that has been used for linkage mapping in our previous studies, and several QTLs with a high explanation of phenotypic variation have been identified [4,5]. Soybean treatment for phosphorus-deficient and -sufficient conditions were followed as previously described [5,11].…”

“…Despite great efforts, it remains challenging to pinpoint P-efficiency genes underlying previously identified QTLs that have a relatively large confidence interval. Here, we expanded our previous studies [4,5,9] by conducting a transcriptome analysis of a low-P tolerant Nannong 94-156, under phosphorus-deficient and -sufficient conditions. Our objective was to better understand the genetic and molecular basis of low-P tolerance in soybean.…”

“…It has been demonstrated that the growth and development of soybean require more P compared with other crops such as rice, corn, and wheat [3]. In soybean, low-P stress may decrease soybean nodule development, increase flower/pod abscission, and impair overall plant growth, consequently limiting yield and seed quality [4][5][6][7]. Thus, low-P stress is more problematic than other nutrient deficiencies or toxicities in soybean [8].…”

“…Knowledge of a genetic and molecular basis of soybean resistant to low-P stress, thus far, has been obtained from the identification of a number of quantitative trait loci (QTLs) associated with P-efficiency [4,5,9] and a major gene, GmACP1, encoding an acid phosphatase involved in regulating P efficiency [5]. Despite great efforts, it remains challenging to pinpoint P-efficiency genes underlying previously identified QTLs that have a relatively large confidence interval.…”