Quantitative trait loci segregating in crosses between New Hampshire and White Leghorn chicken lines: III. Fat deposition and intramuscular fat content

Abstract

In this study, a genome scan was performed to detect genomic loci that affect fat deposition in white adipose tissues and muscles in 278 F (2) males of reciprocal crosses between the genetically and phenotypically extreme inbred chicken lines New Hampshire (NHI) and White Leghorn (WL77). Genome-wide highly significant quantitative trait loci (QTL) influencing fat deposition in white adipose tissues were found on GGA2 and 4. The peak QTL positions for different visceral and subcutaneous white adipose tissues were located between 41.4 and 112.4 Mb on GGA2 and between 76.2 and 78.7 Mb on GGA4, which explained 4.2-10.4% and 4.3-11.6% respectively of the phenotypic F (2) variances. Contrary to our expectations, the QTL allele descending from the lean line WL77 on GGA4 led to increased fat deposition. We suggest a transgressive action of the obesity allele only if it is not in the genetic background of the line WL77. Additional highly significant loci for subcutaneous adipose tissue mass were identified on GGA12 and 15. For intramuscular fat content, a suggestive QTL was located on GGA14. The analysed crosses provide a valuable resource for further fine mapping of fatness genes and subsequent gene discovery.

Quantitative trait loci segregating in crosses between New Hampshire and White Leghorn chicken lines: IV. Growth performance

“…) and fat deposition (mass of white adipose tissues included the mass of adipose tissue around the heart, retroperitoneal, peritoneal, mesenteric, gonadal and subcutaneous) (Nassar et al . ). Body weights of all chickens were recorded at hatch and at 5, 10, 15 and 20 weeks.…”

“…; Nassar et al . , ). For QTL mapping, we used the least squares method of Haley & Knott () implemented in the gridqtl program (http://www.gridqtl.org.uk/; Seaton et al .…”

“…S3; Nassar et al . , ), we performed additional analyses including body weight in the model as a covariate to assess the relationship between these phenotypes at this locus (Fig. ).…”

Lower Expression of SLC27A1 Enhances Intramuscular Fat Deposition in Chicken via Down-Regulated Fatty Acid Oxidation Mediated by CPT1A

“…Therefore, chicken IMF accumulation is dependent on the transport and uptake of blood lipids as well as lipogenesis subsequently in muscle rather than de novo fatty acids synthesis (Griffin et al, 1987). Previous studies have identified about 20 quantitative trait loci (QTL) related to chicken IMF, which are mainly located on chromosomes 1, 2, 5, 23 (Jennen et al, 2005; D'Andre et al, 2010; Ye et al, 2010; Jia et al, 2012; Liu et al, 2013; Nassar et al, 2013; Sun et al, 2013; Zhang T. et al, 2015). Otherwise, a large number of genes including GPAT1, ACC, CD36, AGPAT1 , and DGAT2 (Jeong et al, 2012), FABP (Ye et al, 2010; Serao et al, 2011), LPL (Zhang X. D. et al, 2015), DGAT1 (Li et al, 2013) were recognized as candidate genes for IMF, but their molecular mechanisms affecting IMF are still unclear.…”

Identificação de polimorfismos em região do cromossomo 3 da galinha associado ao desempenho de deposição de gordura

“…A maior parte da gordura na carcaça em galinhas está depositada sob pele, nos órgãos e na porção abdominal (NASSAR et al, 2012). Aves com deposição excessiva de gordura podem causar problemas no processamento da carne e também uma rejeição por parte dos consumidores, preocupados com a qualidade nutricional dos cortes ZHOU et al 2007).…”