Quantitative trait locus mapping of osmotic stress response in the fungal wheat pathogenZymoseptoria tritici

Abstract

Osmotic stress is a ubiquitous and potent stress for all living organisms. Fungal molecular responses to salt stress in well studied model yeast species highlighted the importance of the conserved HOG MAP kinase pathway. However, fewer studies have investigated the genetic basis of salt tolerance in filamentous fungi. The main aim of this study was to identify regions of the genome associated with tolerance to potassium chloride in the wheat pathogen Zymoseptoria tritici. A secondary aim was to identify candidate genes affecting salt tolerance within the most promising chromosomal regions. We achieved these aims with a QTL mapping study using offspring from two crosses grown in vitro in the presence or absence of osmotic stress imposed by 0.75M KCl. We identified significant QTLs for most of the traits in both crosses. Several QTLs overlapped with QTLs identified in earlier studies for other traits and some QTLs explained trait variation in both the control and salt stress environments. A significant QTL on chromosome 3 explained variation in colony radius at 8 days post inoculation (dpi) in the KCl environment as well as KCl-tolerance affecting colony radius at 8 dpi. The QTL peak had a high LOD and encompassed an interval containing only 36 genes. Six of these genes present promising candidates for functional analyses.