Quantitative trait locus mapping of fitness-related traits in <i>Drosophila melanogaster</i>

Wayne, Marta L.; Hackett, Justin; Dilda, Christy L.; Nuzhdin, Sergey V.; Pasyukova, Elena G.; Mackay, Trudy F. C.

doi:10.1017/s0016672300004894

Cited by 61 publications

(56 citation statements)

References 31 publications

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“…Further study of the genetic architecture of ovariole number in Drosophila spp. has revealed genotype-by-environment interaction (Starmer et al 1998;Bergland et al 2008) and several loci of large effect that interact epistatically (Wayne et al 2001;Richard et al 2005;Orgogozo et al 2006).…”

Section: Discussionmentioning

confidence: 99%

The Genetic Basis of Transgressive Ovary Size in Honeybee Workers

et al. 2009

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Ovarioles are the functional unit of the female insect reproductive organs and the number of ovarioles per ovary strongly influences egg-laying rate and fecundity. Social evolution in the honeybee (Apis mellifera) has resulted in queens with 200-360 total ovarioles and workers with usually 20 or less. In addition, variation in ovariole number among workers relates to worker sensory tuning, foraging behavior, and the ability to lay unfertilized male-destined eggs. To study the genetic architecture of worker ovariole number, we performed a series of crosses between Africanized and European bees that differ in worker ovariole number. Unexpectedly, these crosses produced transgressive worker phenotypes with extreme ovariole numbers that were sensitive to the social environment. We used a new selective pooled DNA interval mapping approach with two Africanized backcrosses to identify quantitative trait loci (QTL) underlying the transgressive ovary phenotype. We identified one QTL on chromosome 11 and found some evidence for another QTL on chromosome 2. Both QTL regions contain plausible functional candidate genes. The ovariole number of foragers was correlated with the sugar concentration of collected nectar, supporting previous studies showing a link between worker physiology and foraging behavior. We discuss how the phenotype of extreme worker ovariole numbers and the underlying genetic factors we identified could be linked to the development of queen traits.T HE number of ovariole filaments per ovary is an important female reproductive character that affects fecundity across insect taxa (Richard et al. 2005;Makert et al. 2006). Social insect lineages have evolved a strong dimorphism in ovariole number between reproductive and nonreproductive castes. For example, while most families of bees consistently have 6 total ovarioles, and most species in the family Apidae have 8, the highly social species in the genus Apis (the honeybees) have queens that can have .360 total ovarioles and workers that often have ,10 (Winston 1987;Michener 2003). This queen-worker dimorphism is of primary importance because it translates into differential reproductive potential that defines the social roles of these female castes (Winston 1987) and classifies social species in general (Sherman et al. 1995). Furthermore, ovary size (i.e., ovariole number) is the most sensitive indicator of caste-specific development in honeybees (Dedej et al. 1998). The extreme increase in ovariole number for queen honeybees enables high egg-laying rates (.1500 per day) and is apparently a result of selection for increased colony reproduction (growth and fission by swarming) (Seeley 1997). Honeybee queens are thus highly specialized for egg laying, similar to queens of several other social insect taxa, such as army ants or higher termites (Hö lldobler and Wilson 1990). Honeybee workers in contrast, do not normally reproduce but perform all other essential activities including foraging for nectar, pollen, and water; caring for brood; and building, main...

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Section: Discussionmentioning

confidence: 99%

The Genetic Basis of Transgressive Ovary Size in Honeybee Workers

et al. 2009

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“…This analysis is similar to factorial ANOVA, commonly used in Drosophila genetics to study sex-dependent effects of loci (22)(23)(24)(25)(26)(27)(28)(29)(30). In our case, sex and strain were two discrete covariates.…”

Section: Discussionmentioning

confidence: 99%

“…7). We used approaches developed in Drosophila complex trait genetics, where multiple sex-specific QTL have been described (22)(23)(24)(25)(26)(27)(28)(29)(30), to look for a sex-specific effect of the Idd loci in the current study. A sex-by-strain interaction effect of T1D incidence was only statistically significant when NOD and NOD.NOR-Idd4 mice were compared ( p ϭ 0.0194), supporting the idea of sex-specific behavior of alleles at the Idd4 locus.…”

Section: Alleles At the Idd4 Locus Display Sex-specific Effectmentioning

confidence: 99%

Sex-Specific Effect of Insulin-Dependent Diabetes 4 on Regulation of Diabetes Pathogenesis in the Nonobese Diabetic Mouse

Ivakine

Fox

Paterson

et al. 2005

The Journal of Immunology

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Many human autoimmune diseases are more frequent in females than males, and their clinical severity is affected by sex hormone levels. A strong female bias is also observed in the NOD mouse model of type I diabetes (T1D). In both NOD mice and humans, T1D displays complex polygenic inheritance and T cell-mediated autoimmune pathogenesis. The identities of many of the insulin-dependent diabetes (Idd) loci, their influence on specific stages of autoimmune pathogenesis, and sex-specific effects of Idd loci in the NOD model are not well understood. To address these questions, we analyzed cyclophosphamide-accelerated T1D (CY-T1D) that causes disease with high and similar frequencies in male and female NOD mice, but not in diabetes-resistant animals, including the nonobese diabetes-resistant (NOR) strain. In this study we show by genetic linkage analysis of (NOD × NOR) × NOD backcross mice that progression to severe islet inflammation after CY treatment was controlled by the Idd4 and Idd9 loci. Congenic strains on both the NOD and NOR backgrounds confirmed the roles of Idd4 and Idd9 in CY-T1D susceptibility and revealed the contribution of a third locus, Idd5. Importantly, we show that the three loci acted at distinct stages of islet inflammation and disease progression. Among these three loci, Idd4 alleles alone displayed striking sex-specific behavior in CY-accelerated disease. Additional studies will be required to address the question of whether a sex-specific effect of Idd4, observed in this study, is also present in the spontaneous model of the disease with striking female bias.

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“…The first QTL on 3L overlaps with QTLs for ovariole number (Wayne et al, 2001) and sternoplural bristles (Gurganus et al, 1998). The last QTL overlaps with QTLs for male life span (Nuzhdin et al, 1997) and abdominal bristles (Gurganus et al, 1998).…”

Section: Discussionmentioning

confidence: 99%

“…The use of RI lines derived from parental strains that are not significantly different for the trait in question, has been quite successful in D. melanogaster. The same RI lines used here have been used in QTL studies of life history and morphological traits, which typically show limited interspecific variation, including longevity (Nuzhdin et al, 1997;Vieira et al, 2000), bristle number (Gurganus et al, 1998), reproductive success Wayne et al, 2001), sex combs (Nuzhdin and Reiwitch, 2000) and ovariole number (Wayne et al, 2001). We ask if the RI lines vary significantly in mean IPI and analyse the genetic basis of this variation.…”

Section: Introductionmentioning

confidence: 99%

Quantitative trait loci affecting a courtship signal in Drosophila melanogaster

2002

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Courtship plays a major role in the sexual isolation of species, yet the genetics underlying courtship behaviour are poorly understood. Here we analyse quantitative trait loci (QTL) for a major component of courtship song in recombinant inbred lines derived from two laboratory strains of Drosophila melanogaster. The total variance among lines exceeds that between parental strains, and is broadly similar to that seen among geographic strains of the Cosmopolitan form of this species. Previous studies of the quantitative gen-

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Quantitative trait locus mapping of fitness-related traits in Drosophila melanogaster

Cited by 61 publications

References 31 publications

The Genetic Basis of Transgressive Ovary Size in Honeybee Workers

The Genetic Basis of Transgressive Ovary Size in Honeybee Workers

Sex-Specific Effect of Insulin-Dependent Diabetes 4 on Regulation of Diabetes Pathogenesis in the Nonobese Diabetic Mouse

Quantitative trait loci affecting a courtship signal in Drosophila melanogaster

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