Quantitative Ultramorphological Analysis of Human Sperm: Fifteen Years of Experience in the Diagnosis and Management of Male Factor Infertility

Bartoov, B.; Eltes, F.; Reichart, M.; Langzam, J.; Lederman, Hanni; Zabludovsky, N.

doi:10.1080/014850199262698

Cited by 18 publications

(9 citation statements)

References 21 publications

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“…, 1990), sperm morphology has consistently been the best indicator of male fertility (Kruger & Coetzee, 1999). However, light microscopy (LM) cannot identify the entire variety of morphologic defects that occur in sperm organelles, head structures, or tail organisation (Zamboni, 1991; Bartoov et al. , 1999).…”

Section: Introductionmentioning

confidence: 99%

DNA sperm damage correlates with nuclear ultrastructural sperm defects in teratozoospermic men

et al. 2011

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Sperm morphology has consistently been the best indicator of male fertility. Transmission electron microscopy currently provides the most information on the subcellular details of sperm structure. Recently, assessment of sperm DNA damage has been employed to assess fertility potential. The purpose of this work was to link sperm DNA damage, evaluated by an intercalated fluorescent dye, with the structural characteristics of sperm. Conventional semen analysis was performed on samples from men undergoing fertility evaluation. Thirty men were evaluated and assigned to three subgroups based on strict criteria for sperm morphology: normal morphology (>14% normal forms), intermediate morphology (5-14% normal forms), and poor morphology (<5% normal forms). By quantifying acridine orange-positive cells and ultrastructural sperm defects, we found that the poor morphology pattern group showed a positive association between sperm carrying damaged DNA and the percentage of sperm nucleus with vacuoles (P = 0.01). No statistically significant correlations were established in other ultrastructural characteristics of sperm, including immature chromatin, lytic changes, or abnormal sperm tails. These results suggest that zones without chromatin in the sperm nucleus reflect underlying chromosomal or DNA defects in severe teratozoospermic men. This association should be considered in the evaluation of male fertility.

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Section: Introductionmentioning

confidence: 99%

DNA sperm damage correlates with nuclear ultrastructural sperm defects in teratozoospermic men

et al. 2011

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“…НХ, выявляемый в эякулированных сперматозои-дах, стали называть незрелым именно потому, что его морфология идентична таковой поздних удлиненных сперматид [68,69]. У млекопитающих при созревании сперматозоидов хромонемная структура (фибриллы толщиной около 40-50 нм) выявляется в поздних спер-матидах при наличии большого количества гистонов.…”

Section: корреляционная электронная микроскопияunclassified

“…Показано, что у фертильных мужчин содержание сперматозоидов с не-зрелым хроматином не превышает 30 % [26,53,69,72].…”

Section: корреляционная электронная микроскопияunclassified

Abnormal Chromatin Condensation in Spermatozoa and Dna Fragmentation in Spermatozoa: Is There a Correlation?

Брагина¹,

Arifulin²,

Лазарева³

et al. 2017

Androl. genit. hir.

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“…The MSOME selection criteria are based on an arbitrary descriptive approach taken from different studies that assess sperm morphology through transmission electron microscopy and scanning electron microscopy. 27,28 The microscopic examination of each sperm sample involves the evaluation of six subcellular organelles: (i) acrosome; (ii) post-acrosomal lamina; (iii) neck; (iv) mitochondria; (v) principal piece of the tail (or flagellum); and (vi) nucleus ( Figure 1). With regard to the sperm head, three parameters should be taken into account: the shape, the presence of vacuoles and the base.…”

Section: Selection Criteriamentioning

confidence: 99%

Focus on intracytoplasmic morphologically selected sperm injection (IMSI): a mini-review

Monte¹,

Murisier²,

Piva³

et al. 2013

Asian J Androl

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Intracytoplasmic sperm injection (ICSI) is the recommended treatment in many cases of male-factor infertility. Several studies have demonstrated a positive correlation between optimal sperm morphology and positive ICSI outcomes. In fact, spermatozoa with severe abnormalities of the head are well documented to be associated with low fertilisation, implantation and pregnancy rates. However, a spermatozoon which is classified as 'normal' by microscopic observation at low magnification could contain ultrastructural defects that impair both the fertilisation process and embryonic development. The intracytoplasmic morphologically selected sperm injection (IMSI) procedure changed the perception of how a spermatozoon suitable for injection should appear. Sperm selection is carried out at ×6000 magnification, allowing improved assessment of the sperm nucleus. Currently, standardized clinical indications for IMSI are lacking and the candidates are selected on the grounds of their medical history or of a careful analysis of the sperm suspension. Further prospective randomized studies are needed to confirm the advantages of IMSI in specific groups of patients. In addition to providing a brief overview of the IMSI procedure, this study aims to review the literature, which explains the theoretical basis and the clinical outcomes of this technique. Several reports show that IMSI is associated with improved implantation and clinical pregnancy rates as well as lower abortion rates when compared to ICSI. Although a possible correlation between the sperm's abnormal nucleus shape, increased DNA fragmentation and negative laboratory and clinical outcomes has been long investigated, the results are conflicting.

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Quantitative Ultramorphological Analysis of Human Sperm: Fifteen Years of Experience in the Diagnosis and Management of Male Factor Infertility

Cited by 18 publications

References 21 publications

DNA sperm damage correlates with nuclear ultrastructural sperm defects in teratozoospermic men

DNA sperm damage correlates with nuclear ultrastructural sperm defects in teratozoospermic men

Abnormal Chromatin Condensation in Spermatozoa and Dna Fragmentation in Spermatozoa: Is There a Correlation?

Focus on intracytoplasmic morphologically selected sperm injection (IMSI): a mini-review

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